FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003628745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGATCATACACTGTTCACGTGTCTGACCATGCACTGATCACGTGGCTG27.142857142857142No Hit
GAGATTCAGATTGCTTGAAGATTGGGCTTATCTACAGAACCTTTTCCTAA27.142857142857142No Hit
CTGGTCCACCCGGCCGTGGCACTCGGCGCACCCCACCCCCTTGGCCACGT27.142857142857142No Hit
CCACTGGAATTCTATCACTGCTGGGAGCCAGTGAGGAACTCCGCCCATGA27.142857142857142No Hit
ATCAATGACCGGCCGCCAAACGTCTTTCATCCGAACGATCAAATAGATGA13.571428571428571No Hit
GCATTGGAGGAGGCTGCAAGGATAGGCCAGGGAAGCATCCAAAGGAACAA13.571428571428571No Hit
GTTCTTTGCTTCCACTCGAGATGAATGCCTGTCTCCCCGGGTGCGTCTGG13.571428571428571No Hit
CTTTGCACCGTATTTATAACGGCTTTGTTTACGGTCATTAACACCCGCAG13.571428571428571No Hit
GTCCTGCGGTAGATTGGGTCCGGAGGGCAAAATGAAGCGGCTTATCGCGC13.571428571428571No Hit
CGAGTGCATCGACCAGTGCAACGAGATCATTCGTGACGAAGTGCAGGGCG13.571428571428571No Hit
GTGGGTTCTTTACCACTAACACTACCTGGGAAGCCCTAAATTCTATTTAA13.571428571428571No Hit
GGTTTTAGAAAAGGCAGAGGAACCAGAGATCAAATTGCCAACATCCGCTG13.571428571428571No Hit
GCCTAGAAAAATAAGTGCTTAGCCAAAGTAAGCACAGTTACATGGATCTA13.571428571428571No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC13.571428571428571No Hit
GAGGAAGAGCCAGAAGGTGGTGATCGTTGCAAGGTTTGCTTTGACTACCG13.571428571428571No Hit
CGCTGATACCGGCTCAGTAGTGGCTGTGGACCACCGTGTTCGTGTCGACC13.571428571428571No Hit
ACGCCGTGCCGCCAATTTCCCTCAGACCGCTTTTGTCCAGAACAGCCGAC13.571428571428571No Hit
TTTCTGTTATGGCCCATTTCTCCCTATCGTGGCCTTGATCGCCGTGGGGC13.571428571428571No Hit
GGACGAAGAGGCGGAAGAGGGGAGGAAGGCCAAACTGGTCGTCAATGAAG13.571428571428571No Hit
CTCCAGGTGGCCCCAAGAGCACAGGTGAAGGTTCAGAGATGCGAGAAAGC13.571428571428571No Hit
CCCACGCAGGCACCAGGTACTAAGGACAAACGCTCCCGATCTGGTCCTGG13.571428571428571No Hit
CGCTGACCACAGACCGTCTCCCCACGTCTTGCTAGACTTCGCTGACGCGC13.571428571428571No Hit
TCCCAGGCTGGTCTCAAACTCCTGGGCTCAAGCAATCTGCCCACCTTGTC13.571428571428571No Hit
GTTTTTTAAAGACAGGGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGG13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)