FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003628746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAAGCAGAAAGTAGGCAGACTCTGGTTTTGGGGGTATATGCTCGAGA27.142857142857142No Hit
CCGGGCTTCCCCCCACGGAGACCTGCATGACCTGCCACCTCTACGTGAAG27.142857142857142No Hit
ATAGAAACGTGATGTGTGCATGATGAGTTACGTGAGCAGAGTATGATCAG27.142857142857142No Hit
GCCTATGTCTTTTCTCCTCCATTTCCCCAAGGCTTAACACATTGCTCAAT27.142857142857142No Hit
GCCCTGAAGGTGGGCTGGGCTGACCTGCCCAACGAAAGGCGGCTTAACCG13.571428571428571No Hit
GACCTCTAGTACCATGCTGTCTTCCCCAGCTCTCACTATAAGGCTGGGTT13.571428571428571No Hit
CTCTGGGAGATGGTGAAGGCCAGGGAAGCCAGATGTGCTAATAATGTCCA13.571428571428571No Hit
AATCTCTGGTGGCCAGTGTCAGTCACTAACTCAACAGATAATTCCTGAGT13.571428571428571No Hit
ACCTTCAAGAACACAGTGTCGTGCTTATCCGCGGAGGTCGTGTAAAAGAC13.571428571428571No Hit
GCTCCTGCACGTCCACCCGCTTGCGGTAGGTGCGGCTTTCCGGGCCGCAG13.571428571428571No Hit
GTGGTGGCGTCAGCCATCACGAAGGGCACGTTGAGCAGCCGCGCCAGCGT13.571428571428571No Hit
GGTGGTGTCATCTGCATATCTGAGGTGATTGAGATTTCTCCCAGCAATCT13.571428571428571No Hit
GGCGGTTTCTGGCCCGGCACTGGCAATCGCCAATCATGCTTTCAGCGATG13.571428571428571No Hit
GGGCAGGAGAATTGCTTCAGCCCAAGAGTTCGAGACCAGCCTGGGCAACA13.571428571428571No Hit
GGGTGGGATTGGCAGGGCAGGCTCAAGCCCCACCCAGCCTGAAAGAGCCC13.571428571428571No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT13.571428571428571No Hit
GCATCAGTGAGTTCACCACGCTGCGCCCCTGCTTGCTGGTGGCACCCACC13.571428571428571No Hit
GGAAGAAGACGGAACGTTGAGTTCTCTTGACGACTTGTTTGACGACCACG13.571428571428571No Hit
AAATAAGAGAGATCTAAAGTTCAGGAGAGATTCTATGAGAGTCTTAAGGA13.571428571428571No Hit
GTTCCGTGCAGCCTATAACCTTTTCATGCCTGTCCTGCACTTTCAGACGG13.571428571428571No Hit
TCCTTATCCAGCAAGAAAGCTGTGGCATCCTTCTTAACTTGAACCAGGTC13.571428571428571No Hit
CGACCCTCCTGTCCAGATCAGGAGGGGAGAAAGGGCTCAGAGGAGGGGGT13.571428571428571No Hit
CAGCGTACCGCTGGAAGACACCGTAAACACTACAGGGTAAACAGAGTTAC13.571428571428571No Hit
CCATCGTCGACGGCGCCGCCGAGACCCCCACGGTCACCGGCACCTACAAG13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)