FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAGGAGGAACAAACTTCTCCAGACGTGGCGCCGCCTTTAAGAACTGT33.1578947368421053No Hit
CTGTTGATCTGTATGAGATTACATTTTTGGCTCTCATTTACTAGTTCCCC22.1052631578947367No Hit
GGCTACGTGACACCACGCCACTGCTGTTTTATGACGAAACGCTGATTGCG22.1052631578947367No Hit
CCATTGCGCGCAGCGCGCGGCGTTGACGATCGATTTGTAGAAGCCCTTGC22.1052631578947367No Hit
GTCCTTGGTGCCCAGTCACGCAGGTTCTCAGCAAGTGGTGGCAGGACATG22.1052631578947367No Hit
AATAAAGACATTTCTAAGAAAGATCATTTTAAAGAAACTGAAATTCTTCT22.1052631578947367No Hit
CACCGGTAAACGTCACCTGGTTCAGCTTCCACTCCGGGGCGCGCATGTCG22.1052631578947367No Hit
GGTAATGCACGCTGAATTCGCCGCGTGACAAGGCCAGGCGCAAGTCAGTC22.1052631578947367No Hit
ATCTCGTTGGCCGAGGGCTTCGTCGAGGACGACACCATCGAGTGCTGGGC22.1052631578947367No Hit
GGAAGGCCGGGATCTCCTTGAGCAGGTCGTTGTCGACCACCTGCTCCTCG22.1052631578947367No Hit
CCGATGCTCCGAAGAACAGGGCCACCGCAGCACCTGCGAAGGAAAGCTCC22.1052631578947367No Hit
CTCCCATGTCGTCCAGGCCACCGCGGCAGGACCGTGGACGTCGGGGTCGT22.1052631578947367No Hit
TCGCGGAGCCGGCCTGATCGCTTAACGGCTTGGGCATGAAGCTCGCCCGG11.0526315789473684No Hit
TTCTTGTGCCTTAGCCTCCCGAGTAGCTGGGATTACAGGCACCCGCCATC11.0526315789473684No Hit
ATCGTCCACGAGTCGGTGGCCGAGGAATTCACAGACACGATGATCGAAAT11.0526315789473684No Hit
GTTTCCATTCCATCGCATTCCATTCCATTCCATTCCCCTACACTCGCGTT11.0526315789473684No Hit
CTCTTGCAGGGCCAGGCTGGAAGACCGGCCCTTTTCTTGGTTGAGTCAAA11.0526315789473684No Hit
CCCATCCCCTGTGGCCAGCTTAGCCAAGGTAATAGTAGCAGAAGAACCTA11.0526315789473684No Hit
CCAAACCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGATTTCACCATC11.0526315789473684No Hit
GAAGAACTGCGTGCGCGACTTGACGTTCTGCTGGTAGAGCGTGTGCAGCA11.0526315789473684No Hit
GTCCGATGCCGGCCCGCCGCCGCACCTCGTCCTTGAGCGGCACGAGGAAC11.0526315789473684No Hit
GTGCTACGCAAGCGCTTCGGCGTCGGCATTGTTCAGCGGCGACGAAAGCC11.0526315789473684No Hit
GGAGATCAGCGCGCCGGTCTTGAGCTGCTGCAATCGCGTGACGCCGTTCA11.0526315789473684No Hit
GGGACAAAAATACCTCCACCTAACATGTTCCTGGGCTTTTGCTTTCCATT11.0526315789473684No Hit
ACGTGACTATCTCCACCCGTGTGCCTCGTGACGGCGGGACCTTGGACTCG11.0526315789473684No Hit
ACTGAATCCTCTCCAGAACCTCCCTCCTGATGGTTCTCCAGCCTGTGTTT11.0526315789473684No Hit
GAGCCGAGACAGCGCCACTGCACTCCAGCCTGGGTGACAGAGCAAGACTC11.0526315789473684No Hit
CGCTGGCGTTGGCACCCTGCAGCTGGCCTTGTTCGGATTCGGACACGCGG11.0526315789473684No Hit
GGCCAGTGAGTGGGGAGAGGACATGCCCGGCTCCAGCAGGAAAGTGCCGC11.0526315789473684No Hit
CTTCTCAACCTGTGCACATATTGGGTGAGATAATTTTTTGTTGTGGCGAG11.0526315789473684No Hit
GTCCCTGGGCCCTATGCACCATCGATGTTCTTCTGGGGCGGCTAACCGGC11.0526315789473684No Hit
GTTTGTCCTTCCCTTCCTACTCTTGTCATGTTCACTGTGAGAAATAGCAT11.0526315789473684No Hit
GGGACAAAAAAACCTCCACCTAACATGTTCCTGGGCTTTTGCTTTCCATT11.0526315789473684No Hit
ACCTTTCTTTTGATTGAGCAGTTTTGAAACTCTCTGTAGAATCTGCAAGT11.0526315789473684No Hit
GTACGACGCGTATTGTTGCGGGTATTGGTATGCCACAAATCTCTGCGATT11.0526315789473684No Hit
GATCACGCCATTGCATTCCAGCCTGGGCAACAAGAGTGAGACTCTCTCTC11.0526315789473684No Hit
ATATGAAATCGAAAAGCAAAATTTTGGGTAATTTAGCAAATTAATACTGT11.0526315789473684No Hit
CCCCAAAACAATTACAATAGTAACATCAAACATCACTGATCACAGATCAC11.0526315789473684No Hit
ATCCAGGCCCGGTCGAACTCCAGGCCGGTCTCGGTCAGCAGCATTTCGGG11.0526315789473684No Hit
ATGCTGCTCGGCACGGCCGGGCAAAAAGCCTGGTTGTAGCGGTTCGAGCG11.0526315789473684No Hit
ACTATAAACTGATGACCATCAAGAGGCTTGTTTGATTTGGGGTCTTTCCT11.0526315789473684No Hit
CTGTAAGTCAGACTTGCTAAGAGTTTTCGTTGGGGAGACGCACACAGGTA11.0526315789473684No Hit
GTTACCTAAACATTAGAGTGAGAGACGGAATCTCACCCTAGACCAAATAT11.0526315789473684No Hit
GAGCTGTAATTGCACCACTGCACTCCAGCCTCGGCGACAGAGTGAGACCT11.0526315789473684No Hit
GGGCATGCACGCTGAATTCGCCGCGTGACAAGGCCAGGCGCAAGTCAGTC11.0526315789473684No Hit
GGAGATCAGCGCGCCGGTCTTGAGCTGCTGCAATCGCGTGACGCCGGTCA11.0526315789473684No Hit
GGTAATTTGTATTTCTATAAAGCGTTTGTACTCGTAGTCATTTTTATTTA11.0526315789473684No Hit
ATTCCATTCCATTCCTTTCCATTACATACCCCTCAGATTGATTCCTTTCC11.0526315789473684No Hit
AGACATGAGTGAGCAGCAAGTTCGGTTACCCGACCGGCTACCATCATGTG11.0526315789473684No Hit
GGGTATCTCTCCAGAAACAGGGCCCATTTTCTAATTTAGCTATGCCCAAA11.0526315789473684No Hit
TCCCTTGTTAGCTGTATTCCTAGGTATTTTATTCAGTTTGCAGTGATTTT11.0526315789473684No Hit
GTACGACGCGTATTGTTGCGGGTATGGGTATGCCACAAATCTCTGCGATT11.0526315789473684No Hit
GCACAGCTACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAACTTGGGAGG11.0526315789473684No Hit
AAGGCAGGAGGACTACTTGAGCCTAGGAGTTCGAAGCTACAGTGAGCCAT11.0526315789473684No Hit
AGGGAAGCTTTTATTTTTTTTTTAGCTTATTTCAATTTTCAAAAGAGGTA11.0526315789473684No Hit
ACCGTAGCCGGTGCATTCAGTGAGCGAGCCGTTGGTGACGAAGACCAGAT11.0526315789473684No Hit
ATTCAACAACACCTTTTTACTGTTTTGTAGCTAAATGAAAAATTATTGTT11.0526315789473684No Hit
GTAATGGATGTATTATTTCAAGAACCCATGGTTCTTTTTCTAATGTTATC11.0526315789473684No Hit
CAGGCCTCGGCCTCCCAAAGTGCTGGGATTACTTATTTTGTTTTTTGTAG11.0526315789473684No Hit
GCCCTGGCGCGCCGGAGGGGACGCCCGCAGGCAGGGCACGGGGAGGCAGC11.0526315789473684No Hit
GCGACGAGCTTGGTGACCACGGTGCGACGTTGCGGGAGGTGCTCGAGCAC11.0526315789473684No Hit
CATATATACACACATATATAGATATACACATACCCATATATACATACATA11.0526315789473684No Hit
ATTTGACCACTGCACCTGGGCCTGGGTGACAGAGTGAGACCCAGTCTCAA11.0526315789473684No Hit
GCTACTGGCACTTGTGAGCAAGAATGGGTCCCACTGTCATTGCACTCTGA11.0526315789473684No Hit
GTTACCTGCACAAGGATCTGATAAGTCAAATGTCCTTCCTCTATCTTACG11.0526315789473684No Hit
GGGAGGCCGGGATCTCCTTGAGCAGGTCGTTGTCGACCACCTGCTCCTCG11.0526315789473684No Hit
GTTGTGTCGCGCAATGTCCCACAAGTCAATGATCCCGCTCCACCGCGAAG11.0526315789473684No Hit
GCTGGCGCTGGGCATGGTCGTCTACAAGTTCTTCTGCCGCTACCTCTGCC11.0526315789473684No Hit
ATTGGACCACTGCACCTGGGCCTGGGTGACAGAGTGAGACCCAGTCTCAA11.0526315789473684No Hit
ATTCTGTGGGGCATCCTGGCGGCGACGGTCCTCGCCATAGTTCTCCAGAT11.0526315789473684No Hit
GGGAGGAGGACGAGGCCCTTGGCATCCTCCCCGAGCCCGGGAGCGGATGT11.0526315789473684No Hit
GGAAACTGAGCCCCCAAAACATTCAGAGGGAGCTATATTACCTGCCCCAT11.0526315789473684No Hit
GTGCACAAGCCCTCTCCCTCCTCTCCCCAGGGAGCCCTGGCTGGGGGCCT11.0526315789473684No Hit
CAGGAAGTAGGGGTAGTCGCCGTCCTTCATGGTGAAGGTGGTCGGCGCGG11.0526315789473684No Hit
GTGCTAGGATTACAGGTGTGAGCCACCATGCCTGGCCCACCAATGAGAAT11.0526315789473684No Hit
ACTTACTCTTGTTTCATAAGCTCGATCAATTCTTTTGTAATCCAAAAATA11.0526315789473684No Hit
ACCATACTGCCGCCGGTGCTAGTGCGTTGGGCACGCTTGATGCATGGGAT11.0526315789473684No Hit
CCACCCAATAGTCCAACACATTGTTTGGGAAAACCACTATGTAAATGAAC11.0526315789473684No Hit
TGAGATTGGTGTCCATTACGTGCCTCACTGAAGTTGAGTTGCTATGACTC11.0526315789473684No Hit
ACACTAACCAGGGCACGATAGAAAGAAACCAGTGTTTCTTTCTAGGGAAA11.0526315789473684No Hit
GTCAATGACAGATCACAACTGTCAGTTGAGGTGGGGTTCAGGCCCTCCTG11.0526315789473684No Hit
ACTGAAAGGATGCACATGTGGTAAAATATGCTTAACTCTTAAACTTTAAG11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)