FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGCGAATTGCTCTCGGAGCCGATGTTTCTCGACGGTCATCGGCTGCA33.1578947368421053No Hit
TCTGAGAAGTGAGAGCAAGAGACTGGAAAAAAGCAAAGAGCCCACCAAAG33.1578947368421053No Hit
GTATTCCTCCCCAACAGTAGCTACTAAAGAGTCTACTCTATTCAACATCA22.1052631578947367No Hit
ATGCGAAGCAGAAGTGCGGCATCATCACGGCGGCGGAGCTCGACGGGAAC22.1052631578947367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22.1052631578947367No Hit
ACGCAGCCCTGCAGCGCGACGTGTCGGCATTGGGCAAGCGCATTGCGCTG22.1052631578947367No Hit
CTGACGAAGAGCGCTCGACCCACCTGCATGCCCCGGGCCGACGAATCTGA22.1052631578947367No Hit
GAATAAATAGAGCCAGGGCACAGTGGCTCACGCCTATTAATCCCAGCACT22.1052631578947367No Hit
CTGCATGACTGGGCACCAAGGATACGGCACACACACGCACCCACCCCACA22.1052631578947367No Hit
GATTACGATAATCTTGAGCTCGCCTGCCAGCATCATTTAATGCTGACACT22.1052631578947367No Hit
CGCTGTACACGACCACCGACAACCGGCCGCGCATGCTCGAGGAGCTGGGC22.1052631578947367No Hit
AAGCTATTCAGGCTGTTTTATCGCAGGATCCCAAAAGGTTCTCCTTTTAT22.1052631578947367No Hit
CTTCGGTGCCTCCGAGGTTTTCCCGGAGCTGTGGAACAAGTTCTGCGAGC22.1052631578947367No Hit
GGGGGGGGCGGCGGCCGGTTGGCGGCCCCGGCGCCGACGGCGGCGGGTCG11.0526315789473684No Hit
ACGCAATGCGATGATGGAACAGGCACTGGAAACCGGAAAGCAGCAAGATG11.0526315789473684No Hit
AGTCAATGTTGTTTAAGGTGTATTAACACTCTGCATTATGAAGCAACACA11.0526315789473684No Hit
GGTCGACGCGCTGCTCCCGGTTCCCGACGACGGACGCCGCCGCCTGTACG11.0526315789473684No Hit
TCACCAAGTCGTTTCTCAGAATTCTTCTGCCTAGTTTTTATTTGAAGATA11.0526315789473684No Hit
ATTACAGGGGAATGGAATGGAATGGAATGCAATGGAATGGAATCATCCGT11.0526315789473684No Hit
GGGGTGGTCGCGGGTGGCTTGTTATCAGCCGGTGGTTGTAATCCCCTCGT11.0526315789473684No Hit
GGGGGGTGTGCATGATTTCATGATGGTGAGATTGGCTTTGTGTATTGGGT11.0526315789473684No Hit
GGGCAGCAGAAAGGCAAATCATGAACGAACTCGCATTCACAATCACTGCA11.0526315789473684No Hit
GGGCGGCGCCCGACGGCCGCTGGGGGTCTCGCAGCGGGACGTTGTGCGGG11.0526315789473684No Hit
ACCTGATTGTAAAAATAGATTGGAATTTTGTCTGCTTTCCTCCTTTTTCT11.0526315789473684No Hit
GGGGGGGGGGGGGGTCCGTGTGGCGGGGTGTGTGTCGGGGGGGGGGGGCG11.0526315789473684No Hit
GGCTGGTGTCCAGACTGCCGTTTTGGCTATCCAAATACAAGCCCGGAACC11.0526315789473684No Hit
GCGAAAAGCAACGCGTGGCGATCGCCCGCACCTTGCTGAAGAGTCCGTCG11.0526315789473684No Hit
GCGCGGCCGGGTCGATGAAGACGTCGCCGTCGCGCAGCTCGACGGAGAAG11.0526315789473684No Hit
GCCCCAATTGCTTTCGCCATATCGCCAGAGAAGCGAATACCACCATCGGC11.0526315789473684No Hit
CTTAAACAATGAATACATTCTCATTATTGAAAAATCACAAAATTAGACAT11.0526315789473684No Hit
CTGCTGGTTCCCTCTGGGGTGCAGGGAGCAGAGGTGCATGCAGGTTCTCT11.0526315789473684No Hit
CCTTCGAGGTACCCGTCTTCGAGTGGCGCGGTCCATCGCCGTACCACTTC11.0526315789473684No Hit
GCCAAAGATTTGTAGCCTCAGATTTTATGTTTCTGAAGGATCATCATCGG11.0526315789473684No Hit
GGGGGGGGGGGGTGGGGGGGTGGGTGGGGTGGGGGGGGGGGGGGGGGGGG11.0526315789473684No Hit
GTACAGGAAAAATTGAATAATTTTGAGCAATATGTAAATTTAAAGGAAAT11.0526315789473684No Hit
GGCGGTCGGCGCGCTGCTCGCGCTGACGGGCGTCCTCGACATGATCATGC11.0526315789473684No Hit
GTAGCACAGTTGTACGGGACGCGAGTGTGGGGCAAGCGCCACATAATTAT11.0526315789473684No Hit
GCCTCTTGAGTAAATCCTGGTTTTATTTCATTCCCATTTTTGTCCCCATT11.0526315789473684No Hit
GCGGGGGGTCGAAGAGGCCCCGGAAGCGCCCGGTAAAGCCGCGGAAGGGC11.0526315789473684No Hit
GGGCGACGCGCGGCTCCCGGTTCCCGACGACGGACGCCGCCGCCTGTGCG11.0526315789473684No Hit
GTAAAATATCCAGACTGCCGTTTTGACTATCAAAATACAAGCCAGGAACC11.0526315789473684No Hit
GGCCTGTAGCTGTCAGGCACCATATCCAGGGTTGCCTCTGGGAGATTTCT11.0526315789473684No Hit
CTCCATCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAGGCTCCCAAGTTGG11.0526315789473684No Hit
ACCTTGATCAGTCTGTGGCAGTTCTCTTGATAGCCTCCTCAGCTAACAGA11.0526315789473684No Hit
TGGTAACCCTGCATCTAGCAAGTCTATTTTTCCAGCAGCACCATTTTTTC11.0526315789473684No Hit
TGCCATTTTCATTCACTTAAAAATTTTTTTTAAAGCCTGACAAATGCATA11.0526315789473684No Hit
GCACCAATTGCTTTCGCCATATCGCCAGAGAAGCGAATACCACCATCGGC11.0526315789473684No Hit
CCAAAGGCAATCATTTTTGATACTAAACTGGAGTACTACGTCTCCTGTTT11.0526315789473684No Hit
GGGGGGGGGGGTCGTGGTAGGCGGCGCCGGGGCGCAGCGCGTCGGTGGAG11.0526315789473684No Hit
CGGTGTACACGACCACCGACAACCGGCCGCGCTTGGTCGAGGAGCTGGGC11.0526315789473684No Hit
GGAAGGGAAATATCAGTTCTAAGAAGCAGACAACTGGACATGCACATTCA11.0526315789473684No Hit
AGACGCACACCTCTTTACACCCAGAACCCCTTTCCCAGCTGCAGCAAGGG11.0526315789473684No Hit
ACAACATCCAGAACAGCCTCGCCACAACAAAAAATTATCTCACCCAATCT11.0526315789473684No Hit
GTCCTTCTGACCGTGACGACGCGGAGCGGCTGGACCTCACAGTGGTTCCG11.0526315789473684No Hit
ATTCAAAAGGTTAAAAGGGGAGAAGAATACAATGCAAAACTTATCAAAAG11.0526315789473684No Hit
GGTTTGGTCTCTGGCTCCGTGAGGTTAGGAATCCTCTTCTCTGGGGAGGT11.0526315789473684No Hit
GGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGTGGGGGGGGGGGGGGG11.0526315789473684No Hit
GGGGGGGTAAGTCGGGTTTGTGAGTTGGATTTTTATGTTGATTAATGCCA11.0526315789473684No Hit
GCGCAGACCTCAGCGTGTACGGCGGCTACCTCGTCTTCACGCCCCAGGGC11.0526315789473684No Hit
GGGGGGCATCGTGGGCAAGGAGGGCACGATCATCGGCCGCAGGGCCGTCA11.0526315789473684No Hit
GGGTCTGTGTGTGTGTGAGCGTGCATGTTGGTTATTTCCTGTAAAGTTCT11.0526315789473684No Hit
GGGGGGTCGAGTGGGCGGGCGGCCTGCGGGGTGGGGGCGGCGACGAGGCG11.0526315789473684No Hit
GAACAGAAGAGGAGGCTGAGCTCTGGAGAAAGAGGGTCACAAAGCGTTAC11.0526315789473684No Hit
CCTGACCTCAAGTGATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTA11.0526315789473684No Hit
GATCCGCCCGCCTCGGCCTCCCAAAATGCTGGGATTACGGGTGTGAGCCA11.0526315789473684No Hit
GGACTCGACACCGGCTGGCGCTGCCTCGAGATCGGCGCCGGAACCGGATC11.0526315789473684No Hit
GCCTCTCTAGTTTTTCCTTTTCCAGCATGCCATGTAGTTGAAACGGTACA11.0526315789473684No Hit
GGGCAATAATATGACAGAATGAAGTATTTTATCTGGCTTTCCGGTATATC11.0526315789473684No Hit
GTATAGGACAAGTCAAAATGTCCAAGCATGTGGTAGATGGTATATGTAAG11.0526315789473684No Hit
GTTACATATTGAACCCGGCAAGCACGCTCGTCAAGGTCTTCGAGCAAAGC11.0526315789473684No Hit
CGCCTGGGCCTCCCAAAGTACTGGGATTACAGGCGTGAGCCACCAAGCCT11.0526315789473684No Hit
CTCCCTACCCCTCCATCACTCTCCACAGGGCTCCCTTGCCATTTTACCTT11.0526315789473684No Hit
CTGGAATTACAGGCGTGAGTCACCGTGCCCAGCCTATTATTTTATTTTTA11.0526315789473684No Hit
GCGGGGGGGTACGGGGGCGCGGGCGTCCGGCCGGGGGCCTGCTGCAGGGT11.0526315789473684No Hit
ACGCCCGCGCCCCGAGGTGGCACCTCCGCGATGCGGGACAATTGCCGCCG11.0526315789473684No Hit
ACCACGCAGACCAGGCACTGGAAGCATTCGACGTACTGAACCTATCCATT11.0526315789473684No Hit
GGGCGCAACAGCTGAACGCACCAGGCCGTCAACTTTTTCGCGGTCACGCT11.0526315789473684No Hit
GTTTTGCCACATTGTCCAGGCTGCTCTCAAACTCCTGGGCTCAAGCAACC11.0526315789473684No Hit
ATTCTCATGTTACTGATATTTTTAGGATGCTGCGGAATCTCTTTTGCGCT11.0526315789473684No Hit
CTTCCCACTCTCCAGCAGTGCTCCCTTGGTCAGCTTCTACATAAACAACT11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)