FastQCFastQC Report
Tue 10 May 2022
EGAF00003628937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131706
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCGCAGGTAATATCTCGTATG21771.6529239366467738No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCGCAGGTAATATCGCGTATG18211.3826249373604846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17201.305938985315779No Hit
ATACACATCTCCGAGCCCACGAGACCCCGCAGGTAATATCTCGTATGCCG11790.8951756184228509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.8686012786053786No Hit
ATACACATCTCCGAGCCCACGAGACCCCGCAGGTAATATCGCGTATGCCG10450.7934338602645286No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10380.7881189923010342No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA8470.6430990235828284No Hit
ACACATCTCCGAGCCCACGAGACCCCGCAGGTAATATCTCGTATGCCGTC7760.5891910770959561No Hit
ACACATCTCCGAGCCCACGAGACCCCGCAGGTAATATCGCGTATGCCGTC7140.5421165322764339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.5185792598666727No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6150.4669491139355838Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA5330.404689232077506No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA4820.3659666226291893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4650.353059086146417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4110.31205867614231697No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT3790.28776213688062807Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA3770.2862436031767725No Hit
CATCTCCGAGCCCACGAGACCCCGCAGGTAATATCTCGTATGCCGTCTTC3600.27333606669400023RNA PCR Primer, Index 37 (95% over 23bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3220.2444839263207447No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA2990.2270207887264058Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2800.21259471853977802No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2790.2118354516878502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2670.20272424946471684No Hit
CATCTCCGAGCCCACGAGACCCCGCAGGTAATATCGCGTATGCCGTCTTC2630.19968718205700575Illumina PCR Primer Index 6 (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA2540.19285378038965575No Hit
TCTCCGAGCCCACGAGACCCCGCAGGTAATATCTCGTATGCCGTCTTCTG2440.18526111187037794TruSeq Adapter, Index 25 (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.18146477761073906No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2300.17463137594338907No Hit
CACATCTCCGAGCCCACGAGACCCCGCAGGTAATATCTCGTATGCCGTCT2270.17235357538760573RNA PCR Primer, Index 37 (95% over 21bp)
TCTCCGAGCCCACGAGACCCCGCAGGTAATATCGCGTATGCCGTCTTCTG2250.1708350416837502RNA PCR Primer, Index 44 (95% over 24bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2170.16476090686832795Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT2150.16324237316447238No Hit
CACATCTCCGAGCCCACGAGACCCCGCAGGTAATATCGCGTATGCCGTCT2120.16096457260868904No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT2110.16020530575676128Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA2030.15413117094133905No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA2010.15261263723748347Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1970.14957556982977238No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA1960.1488163029778446No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1960.1488163029778446Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1920.14577923557013348No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAA1810.13742730019892793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1800.13666803334700012No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1730.13135316538350567Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.12831609797579457Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1680.1275568311238668No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1660.12603829742001124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1640.12451976371615568No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1640.12451976371615568Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1610.12224196316037235No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600.12148269630844458Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA1560.11844562890073346Illumina PCR Primer Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1520.11540856149302232No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1480.11237149408531123No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1410.10705662612181678No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA1360.10326029186217787Clontech Universal Primer Mix Long (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)