FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003628989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047258
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT83130.406055318870411No Hit
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA76050.371472476844638No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG54240.2649397389093119No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC52340.2556590327159547No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT39310.19201292655835267No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA37390.18263452872085492No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT37270.18204837885601133No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG35350.17266998101851355No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG32010.15635547644703304No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG31810.15537856000562703No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC29150.14238557133492702No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC26440.12914835355387547No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG25340.12377531312614239No Hit
GTACATGGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATT24890.12157725113297883No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA23980.11713228132458146No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA23710.11581344412868333No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT23660.11556921501833183No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA23570.11512960261969912No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT23260.1136153821355198No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA22800.11136847432028597No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC22790.11131962849821567No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT22750.11112424520993447No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC21970.10731427108845099No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA21470.10487197998493596No Hit
GAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGAT21360.10433467594216264No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC21330.10418813847595174No Hit
CCATCAGCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTG20640.10081777675310097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)