FastQCFastQC Report
Tue 10 May 2022
EGAF00003628990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047258
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA72870.3559395054262824No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT69000.33703617228507593No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC54360.2655258887741555No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG51810.25307020414622877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48720.2379768451265058No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA41850.2044197653642091No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT37310.18224376214429253No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT36550.17853147966694966No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG30330.1481493783392225No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG30040.14673284949918378No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG29860.14585362470191837No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT27970.1366217643306315No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC27940.1364752268644206No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA27930.1364263810423503No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC26660.1302229616394221No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA26630.1300764241732112No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT26230.12812259129039916No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG25720.12563145436481382No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA25480.12445915463512658No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG23330.11395730289001191No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC22670.11073347863337206No Hit
GTACATGGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATT22500.10990309965817693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22450.10965887054782543No Hit
CCATCAGCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTG21540.10521390073942806No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT21440.10472544251872505No Hit
GAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGAT20920.10218545977106941No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC20810.1016481557282961No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA20780.10150161826208519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)