FastQCFastQC Report
Tue 10 May 2022
EGAF00003628996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910376
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48470.5324173747989842No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG21880.2403402550155101No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG20340.22342416759668532No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC19820.21771224197474454No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG18170.19958786259743228No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG16750.18398991186059388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.18168317266711775No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA16420.18036503598513143No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT16060.1764106259391724No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA14510.15938469379684878No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA14230.15630904153888064No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT14180.15575981792138632No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA13910.15279401038691706No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC13500.1482903767234637No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT12790.1404914013550445No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA12660.13906341994955929No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT12360.13576807824459344No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA12220.13423025211560938No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT11750.12906755011116286No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA11420.1254426742357004No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT11420.1254426742357004No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG10880.11951105916676186No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT10850.11918152499626528No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC10820.11885199082576871No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG10620.11665509635579147No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG10020.11006441294585972No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA9890.10863643154037453No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG9600.10545093455890753No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA9590.10534108983540867No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT9520.10457217677091662No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG9450.10380326370642459No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT9300.10215559285394167No Hit
GTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA9280.10193590340694395No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT9260.10171621395994622No Hit
ATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCC9230.10138667978944964No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC9200.10105714561895304No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT9150.10050792200145874No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG9110.10006854310746328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)