FastQCFastQC Report
Tue 10 May 2022
EGAF00003628999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643362
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18350.2852204513166771Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG11170.17361920660530153No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT10910.1695779359054467No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG9230.14346510984484628No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7850.12201528843792453Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG7770.1207718205302769Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT7730.12015008657645307No Hit
GTCCTGATGATTCTCCTTCAAGGGGACAGTCCTGCTGCCTCCTCTCATGC7380.11470991448049464No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT6800.10569477215004927No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6570.10211980191556232No Hit
CCCTGGGAGACTCTCCCTCCTCGGTCTCTCTGGACCTGGCCTGCTGTGGG6470.10056546703100276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)