FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC86.896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32.586206896551724Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCACACATCGGCTTCGACTGGCCCGGAGTCTGGGTCCACCTGGACATT32.586206896551724No Hit
CTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTC32.586206896551724No Hit
CATGTAATAAGCCTGCTCATAGACCCTGCCAGGCAGCATACCGAGAATCC21.7241379310344827No Hit
CTCATGTACTGCTGGGGCACAGTGAGCAGAGAGGTGCCTGTGTCCACGAT21.7241379310344827No Hit
ATGTGATGGAAATGCCCGAGATATTCAAAGCGATCAACCCTTACTACGCC21.7241379310344827No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGCTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21.7241379310344827Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
ACTTGAGCCCGGTCACGGTGATCTGCACCGCCGGGCCCTTGCCGGCCGAA21.7241379310344827No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA21.7241379310344827Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG21.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8620689655172413No Hit
ATACCACTGCTTCCCCTGTACTCTGCGATGATACCACTGCTTCCCATGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGAACTCTGCGTTGAT10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGCAGTGGTATC10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGATGATACCACTGCTTCCCATGTACTATGCGTTGATAC10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTCTTAGCTTAGAAACAAAGAAAACTCAAAGATTAGAGGTATTTTGGATA10.8620689655172413No Hit
CAAAAAATCCCAAACCAAAACGTAGATGACATGTTTGCAGACTGAGGAGT10.8620689655172413No Hit
ACTTAAAGGAGTGAACTTGCTGGTAGAGCAGCAACCACAAGCGAGAAAGA10.8620689655172413No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTTAGCAGTGAACTTTTAAAAAATTCTTTTACTTTTAACTTCTTGTC10.8620689655172413No Hit
CCCATGTACTCTGCGTTGATACCACTGCTACCCATGTACTCTGCGTTGAT10.8620689655172413Clontech SMART CDS Primer II A (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCCAT10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAA10.8620689655172413Clontech Universal Primer Mix Long (96% over 27bp)
GCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAAC10.8620689655172413No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCCCGGCTTCCCATGTACTCTGCGTTGATCC10.8620689655172413No Hit
GCAGTGGTTTCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.8620689655172413Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGTGAAGCAGTGGTATCAACGCAGAGTACAGGTGAAGCAGTGGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGGATCAACGCAGAGTCCATGGGAAGCAGTGGTA10.8620689655172413Clontech Universal Primer Mix Long (95% over 23bp)
GTTTCACACTAGCAGATTTCAGATTTTAGCACCTTTGAAATGAAATATTC10.8620689655172413No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTTTTGATGGCGTGGCGACCGTTATTTCGAGCCGGATATTCCAGCCTGTT10.8620689655172413No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTTTGATAC10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CTTCATCCCAGACAGCGATCAATCCCAGGTCCGTCATTGGCGCCATGACG10.8620689655172413No Hit
GTACATGGGAAGCAGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAT10.8620689655172413No Hit
CCCATGTACTCTGCGTTGATACAACTGCTTCCCATGTACTCTGCGTTGAT10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGCCAGTAGCTCCCAAACTTGAGCAAGCATCAGAATCATCTGAAAGGGCT10.8620689655172413No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGAACTGGG10.8620689655172413Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGCAG10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTCTCTTA10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGAAGGACGTTTGTGTTGGAAGCCCTGGTATCCCCGGCACTCCTGGATC10.8620689655172413No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGCAGTGGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CCCCCACCGCGATGCCGGCCTGCGGCCCGCCGAGCAGCTTGTCCCCCGAG10.8620689655172413No Hit
CTACACGACACGTACTACGTTGTAACCCACTTCCACTATGTCCTATCAAT10.8620689655172413No Hit
AGGCGTGGATCGGCGCCCGCGACTGGGCGGTGCTGCTGCTGCTCTATGGC10.8620689655172413No Hit
GTGTACGTACAAACCTACTCATAAGGTTACTATGAGGATTGAGTTAATAA10.8620689655172413No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACGCTGCGTTGATACCACTGCTTCCATGTAATCTTCGTTGATACCAAT10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8620689655172413No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGTTACCACTGCTTCCATG10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG10.8620689655172413Clontech SMART CDS Primer II A (96% over 26bp)
GGTCTCCGCCAGGCACCACAGTGGGAGGCTGGTAATTAATGCCAACCTTG10.8620689655172413No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGCGTTGATA10.8620689655172413No Hit
TGCCCAGGAAGGCCGGGTACCCTCCGGAGGGTGCTGAGCCTGCATCTCGC10.8620689655172413No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAGAGTACATGGGAAGC10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGCATGTCACCGTCCGTGTCGAGGTGTGTGATGCTAAGCGTGCGAGTGTC10.8620689655172413No Hit
ACATGGGAAAGCAGTGGTATCAACGCACAAAAAAAAAAAAAAAAAAAAAA10.8620689655172413Clontech Universal Primer Mix Long (95% over 23bp)
GTACATGGGAAGCAGTGGTATCGACGCAAAAAAAAAAAAAAAAAAAAAAA10.8620689655172413No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAATAAAAAAAAAAAAAA10.8620689655172413Clontech Universal Primer Mix Long (95% over 21bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCACATGTACTCTGCGTTGATACCACAG10.8620689655172413Clontech Universal Primer Mix Long (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTCCATGGGAAGCAGTGGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGG10.8620689655172413No Hit
GTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGTTACCACTGC10.8620689655172413Clontech SMARTer II A Oligonucleotide (95% over 23bp)
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGC10.8620689655172413Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGG10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATACCACTCTCCTCCTCTCTCACTACCTCATTCATTTCCTCATTCG10.8620689655172413No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAATCAGTGGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
TTCTAGAACATTCCGCAGCTGTCCTGGGGGATGGAGTTTGCTGCAGGACT10.8620689655172413No Hit
TGATACCACTGCTTCCCATGTACTATGCGTTGATACCACTGCTTCCCATG10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTGATGGCGTGGCGACCGTTATTTCGAGACGGATATTCCAGCCTGTT10.8620689655172413No Hit
CCCATGTACTCTGCGTTGTTACCACTGCTTCCCAGGTACTCTGCGTTGAT10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GTCAATAGCCTCTGTGTATAGTAACAGTTCAGGTAGTAATTTTGGTCGCT10.8620689655172413No Hit
CAGTGGTATCAACGCAGAGTACATGCAAACACTGGTATAAAAGCCATATA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)