FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT86.896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA76.0344827586206895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG65.172413793103448Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG43.4482758620689653Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32.586206896551724Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA32.586206896551724Clontech Universal Primer Mix Long (96% over 26bp)
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC32.586206896551724No Hit
CACCAAACCCTAAGCTGTCTCCTAAAACTTCAGCTGTTTCCACCACCACG32.586206896551724No Hit
GCCGATACCAAACATAAGAAACCATTCGGGCATAATGAGGAAAAAGGGAT21.7241379310344827No Hit
GGGGTAGACGGCCTTGCTCATTCAGATGGCGCGCGCCTCTCTCACCCTCT21.7241379310344827No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT21.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21.7241379310344827Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA21.7241379310344827Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATA21.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
TACTGACACTTCTTTTTTTCCCTTTGCTGGTGTTATTATTATCTTTCATG21.7241379310344827No Hit
GCCATGGCTGGCGCGATCCTCGCGGCGCGCATGGAAAAAATCCCGGTCAT21.7241379310344827No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC21.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 24bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
AAAGAAAGAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT10.8620689655172413Clontech SMART CDS Primer II A (100% over 25bp)
CCTCAACTTACACGCCCAGCGGATTTACCTACCAGACAGTCCACACACTT10.8620689655172413No Hit
AAATACATATGATGCAAGCTGGAAAAGAGAGTGGCTGGCCCATAGCTCCA10.8620689655172413No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAG10.8620689655172413Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTT10.8620689655172413No Hit
GTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCAT10.8620689655172413No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAACACA10.8620689655172413No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTTA10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAGAAAAAAAAAAAAAAA10.8620689655172413Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.8620689655172413Clontech Universal Primer Mix Long (95% over 22bp)
CTGTGTAATTATGCAGAAGAATGGAGCTGGATTACACACAGCAAGTTCCT10.8620689655172413No Hit
CAGCAGGATTGTGGGGGAGGCCCATGGGATAGGGTGCCAGCTCCTGGGGT10.8620689655172413No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.8620689655172413Clontech SMART CDS Primer II A (96% over 26bp)
CCTGTACTCTGCGTTGATACCACTGCTTCCCCTGTACTCTGCGTTGATAC10.8620689655172413Clontech Universal Primer Mix Long (96% over 28bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10.8620689655172413No Hit
GTTGATACCACTGCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCCAGCCAGCCCTAATGTTTATACTCTCCCCCTGGATACTGTCTTGT10.8620689655172413No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATTGGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGTGAAAGAAGAAGAAGGGAAGATGTGGGTGTAAGTGGAGGTGATAATCA10.8620689655172413No Hit
GTCGTGGCGCACACCTGTAATCCCAGCTACTCGGGAAACTGAGGCAGGAG10.8620689655172413No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCGGTGGTGTGTTGCCCAACGGTTGACGACGTCATCACCCTACATGAG10.8620689655172413No Hit
GTACATGGGAAGCAGTGGTATCAAAGCAGAGTACAAGGGGAGCATTGGTA10.8620689655172413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCTTGTCCTCATCCCAAACCTATTGAATTGGAACTCTGAGGGCTGCCAC10.8620689655172413No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8620689655172413No Hit
CGCGCGGGCCATGGGGATTGGGCAGCGTAACTTTCGATCGCGGCGCGAAC10.8620689655172413No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
ACCAAGTCAAGGGTTTTCATTCGAATAATTCAGCCCTGGGTTTTCGTCTT10.8620689655172413No Hit
TAGTTAGACTGCTTTGCTAATTTACATAGCACCCCACATTAGAAAAGATT10.8620689655172413No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTACCCCCGCATCCACTTCCCTCTGGCCACATATGCCCCTGTCATCT10.8620689655172413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTCGTTGTTACCA10.8620689655172413No Hit
GTACATCTCCACACACTGCTCTTTTCCCCGACCTGCGGGCTCCCCTCGGT10.8620689655172413No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACTGCGTTTATACCA10.8620689655172413No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGATTACGAGCGGGCCATGAACGAGAACACCCGCGCGGTCCTGTGGG10.8620689655172413No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8620689655172413No Hit
GGAAAGCAGTGGTATCAACTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8620689655172413Clontech SMARTer II A Oligonucleotide (95% over 21bp)
ATCTGCACCCTACTTCAGATTTCAGTGGGCATTCACACCACCCCCCACAC10.8620689655172413No Hit
CCTCCAACTTGTGGGTGCCCTATGTCTACTGCCAGTGCCAGGCCTGCACC10.8620689655172413No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG10.8620689655172413No Hit
CCTCCAACTTGTGGGTGCCCTCTGTCTACTGCCAGAGCCAGGCCTGCACC10.8620689655172413No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTTTGCGTTGAT10.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph