FastQCFastQC Report
Fri 10 May 2024
EGAF00003629040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Total Bases4.3 kbp
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATAATAGAACCAAAGGGGAAAACGGCGCTTTCAAAAAAGGGAAAGG24.651162790697675No Hit
CTGGCGATCATCGCCGTCGTGCTGCTGCGCTCCTCGGCGCGCCTGCCGAT24.651162790697675No Hit
GCACATTCTTGATAATCCGCTTGCAGGTCTTGGCATCATACCCGGCGGTG24.651162790697675No Hit
CTATTCAGTTGGCCAGAATAAGTCCCATGTGCAGGCCTGGGTTCTACAGG24.651162790697675No Hit
TCTTAGGTGAAGGAAAACGAAGATAATTTGTTGCTATCAAACCTACTCTT24.651162790697675No Hit
CGCTGACTACTCGCAGATCGAGATGCGGATTATGGCGCACGTATCTGGGG24.651162790697675No Hit
CTGATGCGCCGCGCTGCGATCGTCATAGCGCTGATCGTGATCCTGATCGC24.651162790697675No Hit
GTTTAAAATATCTGGATTCGGTTGAAAAAGCGGTTCATTATGTGGTTTTG12.3255813953488373No Hit
GTCTTCCGCTTTTTGGGCGGGCCCCAGGGGGGCGACCAGCAGATCGCCGC12.3255813953488373No Hit
CACATGGAGCTTGTCTTCTGGGTATAGGAATTTCTTGTCCCTTTTGGCAA12.3255813953488373No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTACCCTATTAGAGGTTGTC12.3255813953488373Clontech SMART CDS Primer II A (100% over 21bp)
GTGTATGCCAATATCTGCCCTGATTCATCTTGGAAACTCTGAGTCTTTAG12.3255813953488373No Hit
CTTTCCATCCGGACGTCGGCAGGGACCGTATTGACCAGGTCGCCGCCTTT12.3255813953488373No Hit
GCTCTGGGGAGCGCCGGTCGCGCAGGAGGACGACGCCGAGCGCGCAGTGC12.3255813953488373No Hit
AGGTTAGGTAGTGCTTAGAAGGCAGTTCCAAGCACACAGCTACTATTCTT12.3255813953488373No Hit
GCTCATTCTTTCATTCAACAAATACGTATTGAGTTACTCAGTAACAGATG12.3255813953488373No Hit
CTGACCGGGGCCAGCAGCAGTTTGGTCAGCGGCCAGCGGCGGCCGATCAC12.3255813953488373No Hit
TTTGAGACCAGCTTGGGCAACATAGCAAGACCCCCATTTCTTTTTTTTTT12.3255813953488373No Hit
GTGTATGTCCGCCCCGATTGCGCCCTCGAATAAAATTGGGGCGGGAAGTA12.3255813953488373No Hit
ATCATCATCGAATGGAATCGAATGGAATCATCGAATGGACTCGAATGGAA12.3255813953488373No Hit
GGCCGTTGGGGGTGGGAAGCGCACCCCCAGGACCTGGACCAGCGCCCGTC12.3255813953488373No Hit
GCGTCTCCTGCCCGCCCAGCGCAAAGCGCACGGCCTGCTCCAGCAGGTCG12.3255813953488373No Hit
ATTAAACACAGGAATACATAAATAAAATGCATCAGTATTTTTTACAAATA12.3255813953488373No Hit
GTGCAGGTACTCGTCAAAATCCGACCGGTTGCCCAGCAGCACCGTCACCA12.3255813953488373No Hit
ATCCTAACTACTACCGCATTCCTACTACTCAACTTAAACTCCAGCACCAC12.3255813953488373No Hit
GTGTAGCGGTGGACTTGGCCGGCAGCATCGACTGCCGTGATGAGGCGGCC12.3255813953488373No Hit
GTGCTCGACCAGGTCATCGGCCAGCTCGAAGGCAACTTCGCCGCCCTCAA12.3255813953488373No Hit
CGGTTATACCGTTTTCCGTGACATACCTCGCAGGGCACGTACACATCCGG12.3255813953488373No Hit
GATCTCGACCGTCTCGACCAGCCGCCCGTCGGGGGAGCTGCCGGATACCT12.3255813953488373No Hit
GAACCTCCCGGGGGCTTTGTAAGCTGAGACTCCATTGTACACCTGGTTAC12.3255813953488373No Hit
GCGGGGGTAGCCGATGTAGGGCAGGCACTGGGACACCACCCGAATGAGAA12.3255813953488373No Hit
GTTGCGGCTGACGCCGCTTGCCGTCATCAGGCAGGGCACCGACTTGCTGG12.3255813953488373No Hit
CATCAGTTCTAGCCTGGATTGGTGGCAGGAGACGTTGGGCGGGCATCCGC12.3255813953488373No Hit
GGACAAACCCTGCGGTTCCCAGGCTGAGAATTTTTTTCACCCGCCGGGCT12.3255813953488373No Hit
CTCCGACGCCCAGCGGTCCGCGCGCAGGACGTAGCCGCCGCCGTTCGGCG12.3255813953488373No Hit
GACCTCCGAGGAAGAGTTTGACCAACTTCGAAAGGAGGCAACAGCCAAAG12.3255813953488373No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)