FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT88.791208791208792Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77.6923076923076925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG55.4945054945054945Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC44.395604395604396Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC44.395604395604396Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA33.296703296703297Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG33.296703296703297Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAATTCCATTCCAGAAAAGCCACAGTTTTTACATGTTCTTGACTTTT22.197802197802198No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
AAGTACCGGTCTGTGAGCTTCCCCTCCCCCGAGTTGCCTGGATGACCCCG22.197802197802198No Hit
AGCAGAGACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCCGAAC22.197802197802198No Hit
CTCCAGGGTAAAGGTCGAACCCAGGCCTTCGCGGCTTTCACCGCGCAGCT22.197802197802198No Hit
AAATCACGAAGTCGATGGAACGGCTCTTCCAGATGGGCGAGAAATACGGG22.197802197802198No Hit
GACGTCGCCCTGCTCGAACAGCACCACGGGCAGCTCCACCGCAGCTGCCT22.197802197802198No Hit
CTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGA11.098901098901099No Hit
GGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTGGTATATGGTTAGT11.098901098901099No Hit
GGGTGACATTGTGCAGAACTGTAGTCTGGAAGACTCACAGATTGAGAAGG11.098901098901099No Hit
GCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCG11.098901098901099No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTA11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
ATCTTTGGTAAGACTTCCTACGAAAACATCGTAAATGCCGCTTCTTCCAT11.098901098901099No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAAAACACAACTTCACAATTTCTCAGC11.098901098901099No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGAGTGGTATCAACGCAGAGTACATGGGAAGCA11.098901098901099Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGT11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GCTGAGTATGGTGTGTATGTGAAGGTGACTTCCATCCAGGACTGGGTTCA11.098901098901099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.098901098901099No Hit
CTTCTCGAGCTCCGGGCCCGCGGCAACCTCGGGCGCTGTTCTGCAGGCCG11.098901098901099No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GTGGGCACCTGCATCGCGATCGCGAGCTCGATCGCGATGCAGGTGCCCAC11.098901098901099No Hit
CCAATATCCTGTTGCAATGAGATTTAACGAATTAATGACAGGAGCAAGAC11.098901098901099No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTGGTTACCACTGC11.098901098901099No Hit
CGCCGTGGAAGGGGCTGGACATGGTGCTGGCTGCATTCCGGATGGTGCGC11.098901098901099No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCTTTTAT11.098901098901099No Hit
GTGGTTCGGACGGGGACGCCTTGGGGCGGGGCTTGGAGTGCTTACTGTTG11.098901098901099No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGTGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.098901098901099No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GACCAGTGTGGGCAGCGCCAAGTATGTGCCGGAGAGAAACGTCGTGATTT11.098901098901099No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAA11.098901098901099No Hit
CCCAGATACACGAAGCGATAGAACCACGTCGCATCGATGCGCCGCGCGAT11.098901098901099No Hit
CTGCTTCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTAATCTGAGTTGATACCACTGCTTCAC11.098901098901099Clontech SMART CDS Primer II A (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAGACCTATGTAGTTCATGAAGTCTTGGCTTTTCGGCGCTGGGTCGC11.098901098901099No Hit
CCGCCCGCACCGCAGGCATGAGCGTTCCCTGGGTGACGAGCCTGGTCATG11.098901098901099No Hit
GTGCCGAGCCGTTATCCTCGTCCTCCGCGCCGCAGACGACTAGCGCCGGC11.098901098901099No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)