FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88.791208791208792Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT88.791208791208792Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG44.395604395604396Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33.296703296703297Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGAGTTCAGGGGAGAGTGCGTCATATGTTGTTCCTAGGAAGATTGT22.197802197802198No Hit
ACCCGGACCGCCGTCAGGAGCCGGCCGGCCGCCGCGGCATTCGCGAGCAA22.197802197802198No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG22.197802197802198Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCCAGTCGAACGCGAAATCTCTTCGCCGATGAGCTGGCCCATTCCACCG22.197802197802198No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
CGGCATTGACAGTGCCCGGCTGGAAATGATGTTCGTCGCCTTCCAGCAAG22.197802197802198No Hit
CCAAAGAACTGGGTACTCAACACTGAGCAGATCTGTTCTTTGAGCTAAAA22.197802197802198No Hit
TAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCAC22.197802197802198No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC22.197802197802198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC22.197802197802198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG22.197802197802198Clontech Universal Primer Mix Long (96% over 26bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGCCAACACCTTCCGTGGCTTTACTCAGCTCCAGACTCTGATACTGCCAC11.098901098901099No Hit
GCTCGGGATCCTCGAGACGTTCGCGGACACGCCGATCGAGGCGGTCAAGG11.098901098901099No Hit
TCCCGGCACTCTCTCCTCCGGTCAGCGGCGCCGACTGGGCTTGGCAACGG11.098901098901099No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAA11.098901098901099No Hit
AGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTT11.098901098901099No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11.098901098901099No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG11.098901098901099No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAGGGTTTTAA11.098901098901099No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.098901098901099Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.098901098901099Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGGGCTGGCCCAGCCAGGACCCTCTACTGCAATGGCCACGTGGGCAA11.098901098901099No Hit
GGCGAGGGAAGCAGAGAGAGTTTCTCTCCCAGCCTATGGAATAAGGAAGA11.098901098901099No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTCTCCTTCGAATACAGAGCCTGTAGCTTGTCCTGCAAAAGCCGTAT11.098901098901099No Hit
ACGCAAAAAAAAAAAAAAAAAAAAGTAAACGGCGTTGATACCACTGCTTC11.098901098901099No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTGTGCGTTGAT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG11.098901098901099No Hit
GGGAAGCAGTGGTATCAACGCAGATACCACTGCTTCCCATGTACTCTGCG11.098901098901099Clontech Universal Primer Mix Long (95% over 24bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTG11.098901098901099Clontech SMART CDS Primer II A (100% over 26bp)
CCCGAAGGCGCTGCCGACCACCCACCACGACGCGGGAAGGCGCGCGGGTA11.098901098901099No Hit
ACGCAAAAAAAAAAAAAAAAAAAATTAAAATCGTTTTAAACACAGCGTAC11.098901098901099No Hit
CGCCTACGTCATTCCGCTGCGGCTCTCGCCGGTGATCTTCACGGCCACCA11.098901098901099No Hit
CTCCTGCAGATATAGGTTTTGCTATAGGAGATATGGGATTTCAATATTTC11.098901098901099No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
AGCTGGGCCATGTAGCGCTCGAAGGAGGGGATGTCCTTGCGTAGGATGCT11.098901098901099No Hit
CCCGGCGCGCAGCGCCCCGAAGAGCAGGCCGGCCAGTACGGTCCCCAGGG11.098901098901099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.098901098901099No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GGTCATAGGCGGGCGTCGCGACGCGCAGTTCGACTTCGGGCATCGCCGCG11.098901098901099No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGT11.098901098901099Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG11.098901098901099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACC11.098901098901099No Hit
CACTTTGCCCTCTTCCAGGCTGAAATCTTGCTTTCAGGCAAGGGCTTCCG11.098901098901099No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.098901098901099No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGATACCACTGCTTC11.098901098901099Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)