FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA125.9405940594059405Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG73.4653465346534658Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT52.4752475247524752Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG41.9801980198019802Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC41.9801980198019802Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41.9801980198019802Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG31.4851485148514851Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC31.4851485148514851Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA31.4851485148514851Clontech Universal Primer Mix Long (95% over 22bp)
GCTATTTCAGCCTAACACACCATTTTTACCACCAGATTACTTATAGGCAC31.4851485148514851No Hit
CGACTGGGCCCAAGGAACGCAAAAAGGTCAACTCGTTTTCGTGATTGTCT31.4851485148514851No Hit
TGTTGGATTTTTAAAAAATTATGTTAGTCACTGGAACTTTTCATTGTGGT20.9900990099009901No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.9900990099009901Clontech Universal Primer Mix Long (96% over 26bp)
GACGAGGTCAGGCTGGTCTTGAACTCCTGACCTCAGGCAATCTGCCCACC20.9900990099009901No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.9900990099009901Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTTTCATGATCTTATTTAAGTAATTTGTTACTGTCAGGGATGCTAAC20.9900990099009901No Hit
CAATCTGCAAATGTAGAAAAATTCAAAACAAAAAAGAAAAGATTCAAACT20.9900990099009901No Hit
GGTATGTCCAGTCTCCATTGGGCTGGGCAGTCTTGTGCGCACTGCTGTGA20.9900990099009901No Hit
GCCTCCCTCTGGGGGGCCCCCATGGGCTTGCTGCTGTCCATCTGTCTATG20.9900990099009901No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.9900990099009901Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.9900990099009901Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.9900990099009901Clontech SMART CDS Primer II A (96% over 26bp)
ATAACCTGCTTTGAGGATACGCTTCATACCCCATATTTCCATTATCGTGT20.9900990099009901No Hit
ATCTGTAGCTCAATTTATAGCCTCTATTCATGGAATTTTGTACCTTCGGG20.9900990099009901No Hit
CTAGTCATCCTCGTCGGTCTTCTGCTTCTTGGTATCGACATCGTCATCCT20.9900990099009901No Hit
CAACGCAGAGTACATGGGGAGCTGTCCTGGGCTGGGTGCCTTGCTCTTTG20.9900990099009901No Hit
TTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGC20.9900990099009901No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.9900990099009901No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.9900990099009901Clontech Universal Primer Mix Long (96% over 26bp)
GAGGTCCAAGTGGGAGGATCACTTTAGGTCAGGAGTTTGAGACCAGCCTG10.49504950495049505No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAG10.49504950495049505No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTG10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGT10.49504950495049505No Hit
ATGCTAACTTCATTCACCTGGTTCAGGTGCTCTCTGCCAGATTCCTTCAC10.49504950495049505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.49504950495049505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.49504950495049505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAGAGTACATGGGAAGCAGTGGT10.49504950495049505No Hit
CCTTGAACTTCGGCTTGTACCATGCCACATGATAGTCCCAGCTCTGAGTT10.49504950495049505No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTAAAAATGGTTC10.49504950495049505No Hit
CCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
CCTTCATGATGGGCGGCACCGAGCTCGTCAGACAGAGAGAGCACCGTCCG10.49504950495049505No Hit
CTCTTTTTGTAGAATCTGCAAGTGGATATTTGGACCACTTTGTGGCCTTC10.49504950495049505No Hit
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAA10.49504950495049505Clontech Universal Primer Mix Long (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGTGGACTAACTTGTCTTTGGACCCTGAGG10.49504950495049505No Hit
GCAGAGAATGGAAAGTCAAATTTCCTGAATTGCTATGTGTCTGGGTTTCA10.49504950495049505No Hit
CCCATGTACTCTGCGTTGACACCACTGCTTCCCATGTACTCTGCGTTGAT10.49504950495049505Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGT10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.49504950495049505No Hit
ACTACAACCTGAGCAAGCGCACCAAGGTCTACGGCTACTTCACCAAGATC10.49504950495049505No Hit
CTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGT10.49504950495049505No Hit
GAGGTTTCCCGTGTTGGGTAAAATTAAGCCGCAGGCTCCACTCCTGGTGG10.49504950495049505No Hit
GAATGGAATGGAATGGAATGGAATGGAATCAACACGAATGGAAGGGAATG10.49504950495049505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACGCTGCGTTGATACCAC10.49504950495049505Clontech SMART CDS Primer II A (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAAAAAAA10.49504950495049505No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG10.49504950495049505Clontech Universal Primer Mix Long (96% over 25bp)
TTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGA10.49504950495049505No Hit
ACACTGACCTGGCAGCGGGATGGCGAGGACCAAACTCAGGACACCGAGCT10.49504950495049505No Hit
GAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGG10.49504950495049505No Hit
GGCTACTGTGCTGCAGTTGGGTGGTGTGTTGGAGCAGACGCTCTCGCTTT10.49504950495049505No Hit
GTCTTGCTCTGTCACCCAGGCTGGAGTACAGTGGCGCGATCTTGGCTCGC10.49504950495049505No Hit
GTCTATAATGGGAAGTGTCACAGTTCGGTATTTCTGTTATGGGTGCCTTT10.49504950495049505No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGGGAAGCAGTGGTA10.49504950495049505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAGA10.49504950495049505No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.49504950495049505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTGTATGTTTGTGGCGGCTGCAGGGGCCTATGTCCATATGGTCACTCA10.49504950495049505No Hit
ACCTCAGCCGAGTGACCACGAAAAAGCTGAGGTAGCCACCAGCAAACACG10.49504950495049505No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGCAGTGGTATCA10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
GCAGCTTGGCAACAGACAAGCAGATCTTACTCAAAAGAAAATGCATGGGG10.49504950495049505No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.49504950495049505Clontech SMART CDS Primer II A (100% over 26bp)
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAGGCGTTTAAAACA10.49504950495049505No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGCGAACACGTGGTCACATC10.49504950495049505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGGGACGAGCGGATCGAGCGGCTCGAGCCTGAGCAGAGCCGACGCAGC10.49504950495049505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.49504950495049505No Hit
GGTCTATAGTGTTCCAGCTTGGGCAGTTGTCATTCCACCGCCCTGTCCAC10.49504950495049505No Hit
GTCCAGCCCAGCTTTCTGGGAAGCATCCACGAAGGATGAATTCTGTATCA10.49504950495049505No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTATAAAAAATCAA10.49504950495049505No Hit
GGTTTGCCGGATGAAGTGGAGGAGCCAGCAAAACTAGAAAACTCTGAGGA10.49504950495049505No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCGGG10.49504950495049505No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.49504950495049505Clontech SMART CDS Primer II A (96% over 26bp)
ATGTGATACCAAGATGAACAGATGGCAGAAGAGGCAACAAATCAAAACAA10.49504950495049505No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
CCAAATATCCAGCTGCAGACATTACAAACTGAGTGTTTCCAAAGTGCTCT10.49504950495049505No Hit
CAAGATCATCGCACCCCCAGAGCGCAAGTACTCGGTGTGGATCGGTGGCT10.49504950495049505No Hit
ATGACAGACAAATGAAAAGATCAGAATTGGCACAGCATAGACAGTCTGTG10.49504950495049505No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.49504950495049505Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCCATGTACTCT10.49504950495049505Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.49504950495049505Clontech Universal Primer Mix Long (95% over 22bp)
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.49504950495049505Clontech Universal Primer Mix Long (96% over 28bp)
CTATTTTTATCCCCATTTCATTCACCCGGAAACTTGCTTCATGAATTTAA10.49504950495049505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAACCC10.49504950495049505No Hit
CCTGAATCGGTACGCGGAGTACCTGCACGGACGCGGCATCGACGACGTCG10.49504950495049505No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT10.49504950495049505No Hit
CTGTAAAATTTCGCCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCGCAA10.49504950495049505No Hit
CATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAGTACTCTGCGTT10.49504950495049505No Hit
CAGCCCTTGTGGGCGTGTCTGGGCGGGGACATGCAAATGAGCAAAGTCGC10.49504950495049505No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTCTCTTATAC10.49504950495049505Clontech Universal Primer Mix Long (96% over 28bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGAACTCTGCGTT10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCACTGGTATCAACGCAGAGTACATG10.49504950495049505Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGTTTAAACACATGTCCATTGAATATTTTTAACTGAAAAGTGAACTATTT10.49504950495049505No Hit
CCAGCTGTAAGGGCACCTTTAAGTCGGGGCCGTTCCGGGTGCGCAACTCA10.49504950495049505No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACAG10.49504950495049505No Hit
CTATATACCATGTCCCCTTAAGCCCTTTTTCAAATAGTAATTCATTTTGT10.49504950495049505No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC10.49504950495049505Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTCCAAATAAATAAA10.49504950495049505No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCGGG10.49504950495049505No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.49504950495049505Clontech Universal Primer Mix Long (96% over 25bp)
ACCACAGACCAGGTAATTTGTAAAGAATAGAAATCTACTTTTCATAGTTA10.49504950495049505No Hit
GGCATAGACACTCCAAGTGACAGAAAGAAAAGTCTGAAAATGTCCCTTCA10.49504950495049505No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.49504950495049505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCCACACTGTTAACATGTCCATTAGCAAAACCATTTTGATGTGATGGA10.49504950495049505No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTGGTTTTTAACACAGGTTACCAA10.49504950495049505No Hit
CGGGAGCCCTGGCCCTGACCCAGACCTGGGCGGGCTCTCACTCCATGAGG10.49504950495049505No Hit
ACTCCAGCCTGGGTGACAGAGTGAGACTCCGTCTCAAAAAAACCAAAAGA10.49504950495049505No Hit
CTCACAGGCATGGAGCAGCATGAGTACATGGACCGTGCCAGGCAGTACAG10.49504950495049505No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTGGAAGTTATAAAAAAGTTGCTCACAAACAATAGTTATTGCCTTTT10.49504950495049505No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.49504950495049505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.49504950495049505No Hit
CATGGGAAGCAGTGGTATCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAA10.49504950495049505No Hit
GTACAGTGGCGTGGGCCTGCTTTCCCGCCAGTGCCCACTCAAAGTTTCTT10.49504950495049505No Hit
GAAACTTATTTGAAGATATTTCCTTTCTCACCATAGGCCTGAAAGCGTTT10.49504950495049505No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG10.49504950495049505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACC10.49504950495049505No Hit
GTACATGGGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTG10.49504950495049505No Hit
GTCCAGAGGATGTATGGCTGCGACGTGGGGTCGGACTGGCGCTTCCTCCG10.49504950495049505No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCGGTTTAACTCA10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
GCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAG10.49504950495049505No Hit
GTACTACACTGAATTCACCCCCACTGAAAAAGATGAGTATGCCTGCCGTG10.49504950495049505No Hit
GGTTTTCTAGATATACAATCATGTCATCTGCAAACAGGGAAAATTTGACT10.49504950495049505No Hit
TCACTGGGCACTGCGAATGCCATGACTGAAGAATTAACAGCCACCCCTCA10.49504950495049505No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.49504950495049505No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTGGCTGACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGACAGATG10.49504950495049505No Hit
CTCTGCTAATGCACTGAGGGTGCAGGTACAATAAGCAACTCCTGCTTATA10.49504950495049505No Hit
CCCCCGGGTGTGCTCTCAGCGCAAGTGGTCCTAGAACAGGATTCTTTTTG10.49504950495049505No Hit
AAACCCTTCCCTTTGTTTTCTTAAGTCATTGTCTCTTTGACATGAGAAGC10.49504950495049505No Hit
GAATGGAATGGAATGGAATGGAATGGAATCAACCCGACTGCAGGGGAATG10.49504950495049505No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10.49504950495049505Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAAC10.49504950495049505Clontech Universal Primer Mix Long (96% over 26bp)
CCTCAGACTCTGCCCTTCTGTGCAAGGGCTAGTGCCCCGGGCTCAGGCAT10.49504950495049505No Hit
GGAACTACCTGCTGCTCGACCGCCCCATCTGGCGCGAGCTTGGGCCGCTG10.49504950495049505No Hit
CTCTTTATTCTTTCTTTCTTTTCCTTCCTTCCTTCCTCTTTCTTTATTCT10.49504950495049505No Hit

[WARN]Adapter Content

Adapter graph