FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG33.0Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33.0Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG33.0Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAAC22.0Clontech SMART CDS Primer II A (100% over 26bp)
TTTCACGTTTTTTAGTGATTTCGTCATTTTTCAAGTCGTCATGTGGATGT22.0No Hit
CTCTTAGTTTTCCTTCATCTGAAAACGTCTTTATTTCACTCTCACTCCTG22.0No Hit
GCGCAAGGCGCCGATGGCCATCAGGTCGTTGCAGACGAACACGGCCGAGG22.0No Hit
CTGCTGGGGCTGGTCTGGGGCCATAAAGCAGGGCTGCCCTGCTTGAGTGA22.0No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA22.0Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT22.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA22.0Clontech Universal Primer Mix Long (95% over 21bp)
AGCTAGAGTGACATTAAAGGACTAATAACTGGATGATGGAGGGAATTGAG11.0No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC11.0No Hit
GGCTTACACTTGTAATCCCAGCACTTTACCAAGCCAAGGTGGGAGGATTG11.0No Hit
ATTTGAGTGGGTGAGTGAGGGGGAGCACATTTCAGCTCTAAACAATATCT11.0No Hit
CTGTAATTCCCGCACTTGAGGAGGCTGAGGCAGGTGGATCACTTGAAGTC11.0No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11.0No Hit
GTTATCGAGTCCGCGGTAGCGCGGCTCGCCGTCGGCATCTTCGGAGCGGC11.0No Hit
ACTGGAACCTACTGTTTCCTGTGGGCTGGGGAGATCAGGGATGCCTGACT11.0No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.0Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGG11.0No Hit
GATTGATATGGCGGCGAAACTGAAAGAGAAGCAGCTGAAGGCGCGTCTGC11.0No Hit
AGTTGGAATGAAGAAAAAGACCGCCCATTTTGTAAAAGGTAGAGATGCTG11.0No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAA11.0Clontech Universal Primer Mix Long (96% over 26bp)
GCTAGCTACCTCCCATTTTTTCTACTTCCTTTTGCAGCAAAAACTCCTCA11.0No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.0Clontech Universal Primer Mix Long (96% over 26bp)
TAAAAATATTCATCACAGCAAAGAGAAAAATGCTGGAAGAAACTTCACTT11.0No Hit
GGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATAATTTTAGTATCT11.0No Hit
CTCCTATTCCAACCTCAATCCCAAATTCATGTGCTTTTCTTTTTTATTGT11.0No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT11.0No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.0Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGCGTCCCCTGTACTCTGCGG11.0Clontech Universal Primer Mix Long (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA11.0Clontech Universal Primer Mix Long (96% over 28bp)
TGCGATAGTTTACTGAGAATGATGATTTCCAATTGTACTTCCTATGTTTT11.0No Hit
GTCGTGTGTCTGCCTGGAGGGGTACTTTCCTTCTTGGGAAAGTGAACACA11.0No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0No Hit
GACTTCGACACGGTGGACGAGGCCCAATTTGCTCGGAATCTTGTTCGAGT11.0No Hit
AAGCCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGAT11.0No Hit
GTCGTATCGCGCTCACCGGGGGCATTGCGTCTGGCAAAACCACGGTGGCT11.0No Hit
GGTCCAAATATCCACTTGCAGATTCTACAAAAAGAGTGTTTGAAAGCTGA11.0No Hit
CCCCATGGTCGACCACGTTCATGACACAATGGGGTGGTGAGCTTCGCACC11.0No Hit
GGCAGAAGGATAGACATAGACAAATGGACAGAATTGAGAGTCTAGAAATA11.0No Hit
ACTAAATTGTGATCTGGCATTTTACCCACTTGCTCACCTTCATCTGATGC11.0No Hit
ACACTGGGCACACCTCACTTCGGTGCGTGGGCGACCAGGAAGAGACTCAT11.0No Hit
GCTGAAAGAATTTGAGAAAAGCACAGAAGCAGGAAAGTCTCTCTGACCTT11.0No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATAAA11.0No Hit
CTTCTTTTCCCCCCACACTCTTGTTCATATCTCTTCTCACTCCAAATGAT11.0No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTC11.0Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCT11.0No Hit
TTCCTGGGAGTATCTGATTGACACAGAGGACGCTGTTGAACTGGGGCCTT11.0No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA11.0Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.0Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.0Clontech Universal Primer Mix Long (96% over 26bp)
TCGCTATGTTGCCCAAGTTGGCCTCCAATTCCTGGGCTCAAGAGGTCCTC11.0No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11.0Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAA11.0No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT11.0No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGC11.0Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG11.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACGTGATGTTGGTTCTGAGGATGATGCTGGTGATTTGCTTGGGAATGGG11.0No Hit
GTGTGAAGTTTCATTTAATACTACTTCCTCATCAGCTGGAAGACCTTGGT11.0No Hit
CACACTCAGCTCAGAAGACCCAGAGAGCTCAGGAGGAGGAGCAGAGGCAC11.0No Hit
GGGCTAGCCCGTCTCCTTAGTTCTTTCTACCACTAGAATTGAGGTTTGAT11.0No Hit
GGCCGACAGAATCGGGGTCAGTGGATCGGTCACTTCTTTTCCTCCACGGG11.0No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTA11.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.0Clontech Universal Primer Mix Long (95% over 22bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC11.0Clontech SMART CDS Primer II A (96% over 26bp)
CTATTAAACTATGGTATTGATTGACATCAGGCTCGCAGTCATGGCCGTTA11.0No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11.0Clontech Universal Primer Mix Long (96% over 25bp)
CCTGTATGGCGCGGAGGTCGTGACCACCGTCACACCGATTGAGCGCAACC11.0No Hit
CAGCACTGATTTCATTTCTGGCAGTGTGTGGGAATACCACTGTGTCAATT11.0No Hit
CGCTGAGGCCGTGCACGGTCCCTGACAGGCTGCCTCCCTCTCCTGCTCTT11.0No Hit
GGGCGAAGCTACCCAAATTGGGAATAAGACTGAAAGCCACAAAGTCAAAA11.0No Hit
ATGTTTGAGGTGTGCAAAGTCCTGTGGTCTGGTTGGCGCCTTATCCCTGG11.0No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATAACCCTGCT11.0No Hit
GTCTCTAAAAAAAACAAATTTAAAAATGGCTGGGCATGCTGGTGCACACC11.0No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACCCTGTCTCTTATACACAT11.0Clontech Universal Primer Mix Long (96% over 28bp)

[FAIL]Adapter Content

Adapter graph