FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAAGGCAGAGTCTCAGTTCTCCTCGGGCTTGTCGGCTGGCGGTCCA31.5384615384615385No Hit
ATGCCCCGCCGTATCGGACTATCCCGCATGCAAACCAGGTTCCACGGAAA31.5384615384615385No Hit
ACTTTTTGGTATCTTCTGTCTGGAGAAGAAGGGATGACATAAACGACTGC31.5384615384615385No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC31.5384615384615385No Hit
AGGCCTTCCAGCTGTTTGACCGAACAGGTGATGGCAAGATCCTGTACAGC31.5384615384615385No Hit
TCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGG31.5384615384615385No Hit
CCATTCCACTCTGGTTGATTCCATTCCACTCCATTCCAATCCATTCCTTT31.5384615384615385No Hit
GTTTCTCTTCTTGCTCTTGCCTGTGACCTTGGCTGGTGTGAGGACTGGAG21.0256410256410255No Hit
TTCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCT21.0256410256410255No Hit
GAACTGGAGCTTAGAAATATTAGTTGGAGACAAATATTTAGAAAGGATCC21.0256410256410255No Hit
ATTGCTATGTGTCTGGGTTTCATCCATCCGACATTGAAGTTGACTTACTG21.0256410256410255No Hit
ACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCAC21.0256410256410255No Hit
GATCAGCCCCTCCTGGCCACAGCTGAATCTCTTCCTGCCCAGTGTTTGCA21.0256410256410255No Hit
CTTTCACGGTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTT21.0256410256410255No Hit
ACTTTGCTGCCCAGTACTCCTCAGGTTAGGTACGGGGACCTCATCACACA21.0256410256410255No Hit
AGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTG21.0256410256410255No Hit
CTCTCGGAGAATGACGAGTGGACCCAGGATAGGGCCAAACCCGTCACCCA21.0256410256410255No Hit
TGGATGTGGTGAACAGGGAACACTTCTACACTGCTGATGGGAATGTAAAC21.0256410256410255No Hit
GCCTCCTCGGCTTATCATGCCCAATGGGGCTTTTTGTTTCTGGACCACTT21.0256410256410255No Hit
TTTTTCTTCAAGGAAGGAAAAAGGAGATGTAAAAAACAAATCCCAACGGA21.0256410256410255No Hit
GTGGATGCAAATCTGAGCGTTCTCAACTTGGTTATTGTAAAAAAAGGAGA21.0256410256410255No Hit
ACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTT21.0256410256410255No Hit
CTCGTAGCCCTCGCGCTTCTCCTCGGCCAATTCGCGGAAGAAGTGGCTCA21.0256410256410255No Hit
AACTACAACTTCCCTTGTAGTCTCTTATATAAGTAGAGTCCTTGGTACTC21.0256410256410255No Hit
GAACTCGAATGGAATCATCACTGAATGTAATCGAATGGAATCATCGAGTG21.0256410256410255No Hit
CAAAAAACAAAAAACAAAACAAAAAAAACCCCAGAATTCCCATGAATCAA21.0256410256410255No Hit
CAATCTCACTGCTCTTGAAGTTTGTGAGCACTTTCAGCACCACGCCCTGG21.0256410256410255No Hit
AACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGC21.0256410256410255No Hit
GCACAGCAGCGACGGCAAGACCTCGAGCTGGCGCCTGACGCTTCCGCTCT21.0256410256410255No Hit
AGCCTGGCCTCTTCCCTGAATCAGCTTCAAGATGGCACCAGCTCTTTGGG21.0256410256410255No Hit
GCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTC21.0256410256410255No Hit
CTTGTCTCTTATCTTGAATTTCTTTGAGTTTCCTCAACACAGGTATTTTG21.0256410256410255No Hit
GTTAACATATATCAGATTGGCAAAAAAAGTAAAAATGCTGAAAATAGATT10.5128205128205128No Hit
ATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGT10.5128205128205128No Hit
TCCAAGTCCCCAGTCACCTTGAGGACTCAGACATCAGGAATTCAGTCCCC10.5128205128205128No Hit
CCGTGTGTCAAGTGTACACCCCCACAAAAAAACCCCACCACGCCCAGTAC10.5128205128205128No Hit
GTATCAACGCAGAGTACATGGGAGGCGATCCTGTGGACAGAGCGCTGGCA10.5128205128205128No Hit
CTCAATGAAGTCACACAAATGGGGGTCATTTTTGTCAGTGGCCAGTTTGT10.5128205128205128No Hit
GCCAGAAGGTGGCCGAGACCCTCAGGCGGCTGCTCAGGCAGTATCTGGAG10.5128205128205128No Hit
GTCGCAGGTCGCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTG10.5128205128205128No Hit
ATTTGACATTCTTGGGAGAAAAATACAGGCAGAACCCTCATTAAGATCCT10.5128205128205128No Hit
TTTTAAAAGATCGCCTTCAAATTATTTTAATCACCTACAACTTTTAAACT10.5128205128205128No Hit
CCTCTTTGCATCTTACAAAGTTAAACAGCTAAAAGAAGTAAAATAAGAAG10.5128205128205128No Hit
AAACTCATTTACTCTAAAAGGTTTTTAATGGATTCCTTAGCATTTTCTAT10.5128205128205128No Hit
GTTGATGCTTAGCTTTTTCAGCTTCCTGTAAAGCCTGTTTCAATTGCTCC10.5128205128205128No Hit
TTGAAATACCTTACCAAGAAATACCTTAAGAAGAACAATCTTCGTGATTG10.5128205128205128No Hit
ATCTCTTGCACTCAAAGTAGAATTATAAAGAAGATCATGTCCATGTTAAC10.5128205128205128No Hit
GTTCAACTCTATGAGTTGAATGCACACATCACAGAGAAGTTTCTGAGAAT10.5128205128205128No Hit
CTGGACTCCAGCCTGGGCAACAGAGCCAGACTCCGTCTCAAAAAAAAAAA10.5128205128205128No Hit
ACTATTTACAGGGGGGACTGCTTCTGAGGCATGACTACGATCACTTCATG10.5128205128205128No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTCTCTCTCACAGTTCAGCGTT10.5128205128205128Clontech SMART CDS Primer II A (100% over 21bp)
ACTATAAACACCTCTATGCAAATAAACTAGAAAATCTAGAAGAAATGGAT10.5128205128205128No Hit
CTCCCAGCCAGCTGTGGTATTCCAAACCAAAAGAAGCAAGCAAGTCTGTG10.5128205128205128No Hit
TTATTAGGCAAAGGAGAATCAGGTAAATTTGAAAAAGCCACTGTGAGAAA10.5128205128205128No Hit
GGCCGACGCCGCACACGGTGCACGGGTCTCCGGGATCGACGCCGCACGGG10.5128205128205128No Hit
GTAGTGAAAGGGTTACTATTGAACATGCTAGGGCTCGGTCACGAGGTGGA10.5128205128205128No Hit
CTCCAGGTATCTGCGGAGCCACTCCACGCACATGCCCTCCAGGTAGGCTC10.5128205128205128No Hit
TCTATTTCGACCGCGATGATGTGGCTCTGGAAGGCGTGAGCCACTTCTTC10.5128205128205128No Hit
GGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGC10.5128205128205128No Hit
GTCTGCACACTACGGCTCACCTCCTATGTCTTCATCTTCATTGGCTGGGC10.5128205128205128No Hit
ATCCAAATGCGGCATCTTCAAACCTCCATGATGCTGCTTACATGTCTCGA10.5128205128205128No Hit
CTTCTCCTCAGCAGCCATTATTGCTTGTTGTTTTTTGGCTTCTTTAGCAA10.5128205128205128No Hit
GAGGTGAAGATGACAGTGCAGGAAGATCGAAAGTGCGAATCTGACTTACG10.5128205128205128No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAA10.5128205128205128No Hit
ATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCT10.5128205128205128No Hit
ATATCTAATTTTGTACTAAATCCTAAGAATTACTCATTAAGAATCTTTAC10.5128205128205128No Hit
TCAGAGCCCTGGGCACTGTCGCTGGACGCAGCCTGAGAGTAGCTCCCTCC10.5128205128205128No Hit
AGTCAAAAGGCCGGGCGTGGTGGCTCACACCTGTAATCCCAGCACTATGG10.5128205128205128No Hit
GGGTAGGAGGATGGGCTCGCCCACCAGTGACTTTTTCTAAGAGCTCCCGG10.5128205128205128No Hit
GCCCAGGTCGCGCGAGCGCTTGATGAAGCGCAGCGTGTGGATGTCGGCTT10.5128205128205128No Hit
GTGCTGTCGATGAGCACGATGCAATTCTTCACCAGGGTCTTGGTACGAAC10.5128205128205128No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA10.5128205128205128Clontech Universal Primer Mix Long (96% over 25bp)
TGTGTGTCCTGATGCATTCATAGGCTACATGCCGAGAAGTTTCCCTGATC10.5128205128205128No Hit
TGCTCCCTCTCCCCCAAGGGCGAGGGAGAAGGAAAGCGTGGTCATGAGGG10.5128205128205128No Hit
CACACACACACACACACACACACACACACACACACACACACACCTGTCTC10.5128205128205128No Hit
GTACATGGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGT10.5128205128205128No Hit
CTACAGAGTCACACTCAATCCTCCGGGCACCTTCCTTGAAGGAGTGGCTA10.5128205128205128No Hit
GCTCTCCGGGGGCAGCTACTTCCGGTCCCCCTGGGAGCTGTCCTGCGGTC10.5128205128205128No Hit
GTGTGACCCACTGGGCACTCTGGTGAGGGAGCTTTCCAGACATCAACAGC10.5128205128205128No Hit
GCCTTAAGTAAAAAGTAGTAAATAAGTAGGTTGGGCATTGTGGCTCATGC10.5128205128205128No Hit
ATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAG10.5128205128205128No Hit
TAACAAGCATTCAATGATAGTTTAAAAAAACAAATTATTTAAAAGTAGGA10.5128205128205128No Hit
CACCAGGTCCGGAAACTTCATCGCATCCTGGATGAAGAACACGGGCATGT10.5128205128205128No Hit
GCTGGGGGCACCCCCCAGCACCATCCTTCCAAGGTCCACCGTGATCAACA10.5128205128205128No Hit
CCTTTCCCCGGAGCTTGAGGCCAATGGCCCGCTCGATTTTGCCAAGCACC10.5128205128205128No Hit
CACCAAAAGCAATGGAAACAAAAGCCCAAACTGACAAATGGGATCTAATT10.5128205128205128No Hit
AATCATCACACTTACCTCATGGGGTTGCTGTGAAGAATACAAGAGTTAAG10.5128205128205128No Hit
GTCGTCACGTTGTCCGGCACCCGGGGGCCTTTATCGAGAGTTGGGTTAGA10.5128205128205128No Hit
GCCCAGCTAATTTTTTGTATTTTTGGTAGAGACGGGGTTTCACCATGTTA10.5128205128205128No Hit
GTAAAGAAGTTTGTACTAAAGATTTCAAAGTAATGTTGATGTTTACGGGA10.5128205128205128No Hit
CGTGTCCACCGTGACCCCTGTCCCCACACTGACCTGTGTTCCCTCCCCGA10.5128205128205128No Hit
TAGGTAAGGAGGTAACTATTTTCTAGTTGTTTTGTAACTTCTTGGTTCCT10.5128205128205128No Hit
GTTGAATGCAGATTCTGAATTAAGTGCCCATTCTGACTCAGTCCAACTGA10.5128205128205128No Hit
ATTTTATAACTTTATTTGATCTGACGATCAGCGATTAGTTCTCATCCACA10.5128205128205128No Hit
TCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTG10.5128205128205128No Hit
CACCTGCTGGGTTCCATCTGCTTCTGTTTTGAGAAGGTCAGTGGATGAGA10.5128205128205128No Hit
AGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTACTCTCTCTTTCT10.5128205128205128No Hit
GGACTGGTGTAACTGCATATTAATTTCTTCTTGTCTTCTTCCCTTTTCCA10.5128205128205128No Hit
GTGCAAAAATTACTGTACCTCAGTAAATTTTTATGTAAAGCAAGAGAAAT10.5128205128205128No Hit
GCAGCGTGTCTGTCTTGGCGTCGCTGTCATAGAGCAGGATGGGCAGCTGC10.5128205128205128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGAAG10.5128205128205128No Hit
GTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTT10.5128205128205128No Hit
ATTTTATTAAGAAAACAAAAATTCAACCTATACAAAAAGGAAAATGCTTC10.5128205128205128No Hit
AAACAGGCCAACAAAACTGCCTCCTCAGGCAGTTCAGACAAAGACAGTTC10.5128205128205128No Hit
GTTACACAAAAGAGGCACACACAAACCATACAGCTTTTCAGACTCCCCAA10.5128205128205128No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA10.5128205128205128No Hit
CTGGTAGCCGTTTATCATGCTCCCTCTCAGGAATCGAACCCTGATTCCCC10.5128205128205128No Hit
GATTTAATGAGCCATTCGCAGTTTCACTGTACCGGCCGTGCGTACTTAGA10.5128205128205128No Hit
CTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAAGCTCTGCCTCCCAGG10.5128205128205128No Hit
GTATCAACGCAGAGTACATGGGACTGGCTGCTCTGAAAAGCCATCTTTGC10.5128205128205128No Hit
GTATACAACAACATGAGCATCACCGTTTCCATGGCAGGTGGAAATACGAA10.5128205128205128No Hit
CTCTTTCTGGCCTGGAGGCTATCCAGCGTACTCCAAAGATTCAGGTTTAC10.5128205128205128No Hit
TCTTTATACTTTTAATAGAGACGGGGTTTCACCGTGTCGGCCCGGATGGT10.5128205128205128No Hit
TTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGT10.5128205128205128No Hit
TGTTAGTATGGAAAAATGCCCTTACGATGGCAAGCTCTATTGAGTAAAAA10.5128205128205128No Hit
CTAGTAGCGGGGCCTGCCGAGGAAGCCGAGTGCCCGCCGCCGCGCCAGCC10.5128205128205128No Hit
GCAATGGGCCGACTGGATCGCCGCCTCGAGCTGGTAGGCGCCGAGCGACT10.5128205128205128No Hit
ATACATCACCTTACGGTTTAGCAGAGACCTGTGTTTTTGATAAACAGTCG10.5128205128205128No Hit
TGTACTACACTGAATTCACCCCCACTGAAAAAGATGAGTATGCCTGCCGT10.5128205128205128No Hit
GAATAATCTAATGGAATTGAATGGAATAATCCATGGACTCGAATGCAATC10.5128205128205128No Hit
AATGGTACCTGGACTTGGTCCTCTATGGGGAATTTTAGAGCTGAAATTTT10.5128205128205128No Hit
GGAGAGAGGCTACCTGAGGGGAAGGAAGCACAAAAGGGACCCGCTGCAGA10.5128205128205128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.5128205128205128No Hit
CCCCACAGGAATAAAAAACACTGGGAAGGGGTAACCCCCTCACCCCCGGG10.5128205128205128No Hit
CGCATGGAATGTAATCGAATGGAATGGAATGGAAGGCAATAGAGTTGAAT10.5128205128205128No Hit
CCCCACATATATGTAAAAGAAATTTGCAAGACTACTGGATTTTGATCTCA10.5128205128205128No Hit
ATAGAGGGCTCAAGGGAAGGGTCCTAAAAGACCAGCCCTGAGCAGCCTGG10.5128205128205128No Hit
GAGTCAGAAGTTTCGGGGTAGTTGCAGCTGCAGTTCTCCTGGCGGTCGGC10.5128205128205128No Hit
GCTTAAGGCTTTAATTAAATTTGGGGGTTCCCTTCTTAGAGCAGCTCGTA10.5128205128205128No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTTTG10.5128205128205128No Hit
GTATTCCAGAGTGAAGCCTTTTAAAAATTCATACAAAGTTGATTGAGGTT10.5128205128205128No Hit
CCCTTTAACTAAGGTGAAGCTGATCAACGAGCTGAATGAACGAGAGGTCC10.5128205128205128No Hit
GAATGGAATCGAATGGAATCATCGAATGGAATTGATCGAAATCATTAACG10.5128205128205128No Hit
GGCCAGGAATCACAAGCTCCGGGTGGATAAGGCAGCTGCTGCAGCAGCGG10.5128205128205128No Hit
AGCCCACTGGGCGAACAGCAACGTTATAACGGCCACTCAGTGGTTCGTCA10.5128205128205128No Hit
ATGATGGACTTACTGTTCACCTTGTCATTAAAACACAAAACAGGCCTCAG10.5128205128205128No Hit
TGCCTGCCCTCCTCCCAAACGGCTCACAGAAATAATTTTTAAAATAAATA10.5128205128205128No Hit
GGCGTGATCTCTGCTCACTGCAACCTCTGTCTCCTCGGTTCTAGCAATTC10.5128205128205128No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC10.5128205128205128No Hit
ACTTTAAGCTGTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTT10.5128205128205128No Hit
GTTGATGCTAGTTCAGAGTATCAGCAAGAGCTGGAGAGGGAGCTTTTTAA10.5128205128205128No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC10.5128205128205128No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.5128205128205128Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCCAGGCAGCGCCCGAGGCCGAGCCCGAGCCCGGCGAGCCACTGCTTC10.5128205128205128No Hit
CTCTGTTGCCCAGGCTGGAGTGCAATAGCGCGGTCTTGGCTCACTGCAAC10.5128205128205128No Hit
ATCCAAAGTTTTAATCAAAGAAGGTGGTGTTCAGTTGCTGCTCACAATAG10.5128205128205128No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT10.5128205128205128No Hit
GTTTAGAGATCGGAAGAGCGTCGTGTGGGAAAGAGTGAGTCTTCGGCGCG10.5128205128205128Illumina Single End PCR Primer 1 (95% over 23bp)
CAGCAGCATTCTCAGAAAGTTCTTTGTGATGATTGCATTCAAGTCACAGA10.5128205128205128No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG10.5128205128205128No Hit
GGCCCATGTCAAGCACCTGTCTGCCAGCCAGAAGGCACTGAAGGAGAAGG10.5128205128205128No Hit
GGTTAAACTCTGTGAGTTGAACGCAGACATCACAAAGGAGTTTCTGAGAA10.5128205128205128No Hit
GTCATTCAGACTGGCCAATTAGAGTCTTAGTGTACTTGGCACTGCCTGAT10.5128205128205128No Hit
ATGTCACTTTCACTCAGTGACAGCCCCTACTCTTCAAGAGCCAACAGCCT10.5128205128205128No Hit
CCTGATGTCGGTGCAGGAGCTGAAGGTGAGTGGAGTGCTGCCAAGAATAT10.5128205128205128No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT10.5128205128205128Clontech SMART CDS Primer II A (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)