FastQCFastQC Report
Wed 11 May 2022
EGAF00003629124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3507981
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT99760.2843801035410397No Hit
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA85990.2451267552475341No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC81350.23189977368748577No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG76600.21835922144390177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65570.18691663381301096No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA49120.14002356341154643No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT47160.13443630395945702No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG46600.13283994411600292No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG42630.12152289308294428No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC42160.12018309107147387No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG41460.11818764126715624No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA40190.11456732519360852No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA38960.11106103482316466No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA37250.10618643601547444No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37120.10581585248038686No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT35160.10022859302829747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)