FastQCFastQC Report
Tue 10 May 2022
EGAF00003629173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201861
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC6190.3066466528948138No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT4970.24620902502216874No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3800.18824834911151733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC3760.1862667875419224No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC3740.18527600675712497No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT3640.18032210283313765No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG3630.17982671244073892No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG3520.1743774181243529No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC3350.16595578145357448No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG3310.1639742198839796No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC3240.16050648713718846No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT3190.1580295351751948No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA3080.15258024085880878No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA3060.15158946007401133No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3040.15059867928921386No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG3000.14861711771961894No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA2940.14564477536522658No Hit
CTTATACACATCTCCGAGCCCACGAGACTTACATCTGCTGATCTCGTATG2900.14366321379563166No Hit
TAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTA2880.14267243301083418No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT2880.14267243301083418No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT2880.14267243301083418No Hit
GTACATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAA2740.13573696751725198No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA2720.13474618673245453No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC2690.13326001555525832No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT2690.13326001555525832No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC2600.12880150202366974No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC2540.12582915966927738No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC2470.12236142692248628No Hit
CCTATTGATAGGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTG2450.1213706461376888No Hit
GTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTAT2400.11889369417569515No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT2340.11592135182130277No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT2310.11443518064410658No Hit
GTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGA2290.11344439985930913No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG2280.1129490094669104No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT2270.11245361907451168No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC2250.1114628382897142No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG2230.11047205750491676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.10997666711251802No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC2210.10948127672011929No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA2200.10898588632772055No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC2150.1065089343657269No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2140.10601354397332817No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT2130.10551815358092946No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC2120.10502276318853072No Hit
TAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG2100.10403198240373325No Hit
GTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAAT2100.10403198240373325No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT2090.10353659201133453No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA2080.1030412016189358No Hit
GTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGG2060.10205042083413834No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG2060.10205042083413834No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC2060.10205042083413834No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC2020.10006885926454342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)