FastQCFastQC Report
Tue 10 May 2022
EGAF00003629187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134625
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA6210.46128133704735375No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4550.33797585886722376No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT4060.3015784586815228No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT4040.3000928505106778No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA2730.20278551532033426No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT2650.1968430826369545No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC2600.19312906220984216No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC2360.17530176415970286No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG2340.17381615598885794No Hit
CTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAA2090.1552460538532962No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC2060.15301764159702877No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1910.14187558031569172No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT1890.1403899721448468No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT1860.1381615598885794No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG1850.1374187558031569No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1850.1374187558031569No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1810.13444753946146704No Hit
GTACATGGGGCTCCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCT1770.13147632311977717No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1750.12999071494893222No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1740.12924791086350976No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1630.12107706592386258No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA1590.1181058495821727No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG1540.11439182915506035No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1520.11290622098421542No Hit
GGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTT1500.11142061281337048No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT1440.10696378830083565No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT1440.10696378830083565No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC1400.10399257195914577No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1400.10399257195914577No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT1400.10399257195914577No Hit
GGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGG1390.10324976787372331No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC1370.10176415970287837No Hit
GCCATGAGGACCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCA1370.10176415970287837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)