FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003629211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA233.33333333333333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC116.666666666666664Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT116.666666666666664Clontech Universal Primer Mix Long (96% over 26bp)
GGGGTACTGATTGCCTGGGCTCAGCATCGTGCTTGCTCTCGATGGGGGTT116.666666666666664No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT116.666666666666664No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)