FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTGGTTACCACTG42.0100502512562812No Hit
CCATTCTCCTGTCGGCGACCTTGGGCCAGCGCTGTCATCCATGGAGCACC42.0100502512562812No Hit
GTGTCCACTGTACTGCTCTTTCTGCCTTTGCGAACCATAGTTTAGCTCTC42.0100502512562812No Hit
CACCACGACGATGAGGACCACCACCACCACCACGATAAGAAGGCGTTTCA31.507537688442211No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA31.507537688442211No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT31.507537688442211No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG31.507537688442211No Hit
GTACTCTTTCTTGCACACACACAGGCCTGCAGTGCCATGTGTGGCTGCCA31.507537688442211No Hit
GTCTGTGAGATGCTAGAACTCCCTTTCAACAGAATTCACTTGTGGCTATT31.507537688442211No Hit
GCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAG31.507537688442211No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA31.507537688442211No Hit
GGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCAT21.0050251256281406No Hit
GGTCAACAGGACTCCCCAAGCGGAATGAGGCCAAGACTGGCGCTGACACC21.0050251256281406No Hit
AGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGC21.0050251256281406No Hit
GGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTGGTTAC21.0050251256281406No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA21.0050251256281406No Hit
GAGTTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGC21.0050251256281406No Hit
TGCAGGCTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCG21.0050251256281406No Hit
GCCCACTCCCATCCTCTCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTG21.0050251256281406No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA21.0050251256281406No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT21.0050251256281406No Hit
GTCCTGGGGAACCCCAAGAGTGATGAGATGAATGTGAAGGTGCTGGACTT21.0050251256281406No Hit
GCATGAGATGGAGGCCTCCTTATTCCCCATCTGGTCCAGTTCCTTCACTT21.0050251256281406No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT21.0050251256281406No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC21.0050251256281406No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCTGGGCGCCACCTCTGC21.0050251256281406No Hit
CACCTGGGCCACTGCCACAGCTAGCGCACCCTTGGGAATCATGGCTTGGA21.0050251256281406No Hit
CTGTGAATGACTACAATCCTCTGATGAAAGATTTCCCTCTGAATGATTTG21.0050251256281406No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC21.0050251256281406No Hit
GCCCAGGTGTTTCGAGGACTTGCTTCTACGGTTTCTTTGTCTGCGGAATC21.0050251256281406No Hit
TGCTTCGGGCATTCCATGCAGCCTTGCGGAACTCTCCCGTCAACACCAAG10.5025125628140703No Hit
CATTACATTTACCTATTTTAATTGTTTACCTAGATTATTTATGATAACTG10.5025125628140703No Hit
CTGCTGATCGCCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAA10.5025125628140703No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC10.5025125628140703No Hit
GTACAGTGGCGTGATCTCGGCTCACTGCAAGCTCCGCCTCTCCTGGGTTC10.5025125628140703No Hit
CGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG10.5025125628140703No Hit
CAGCAAGCCCACGACGTGAGACCCTCGCCACTTCCCTGTGACTGTCCCAT10.5025125628140703No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG10.5025125628140703No Hit
GCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCG10.5025125628140703No Hit
CACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACC10.5025125628140703No Hit
AAGCCCGCGGTGCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGC10.5025125628140703No Hit
GCGTGGTACCTCTGGTGGCGGGCGGCATCTGCCAGTGCCTGGCTGAGCGC10.5025125628140703No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG10.5025125628140703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5025125628140703No Hit
ATCCTCCTTTGGGGAAAGGCTTGGCACACAGCAGGCTTTCAAGACTGTCT10.5025125628140703No Hit
GCAGGGGACCCTTTCTTCTTTGGCTGGTGGGGCTTACCTGGATCAGTAAT10.5025125628140703No Hit
GGGAAGAATTGTCAATGTCTCCAGCATTCTGGGAAGAGTTGCTTTCTTTG10.5025125628140703No Hit
GGGCATGGCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTACATCT10.5025125628140703No Hit
GCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGGACTGCTGTGATTA10.5025125628140703No Hit
CGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGG10.5025125628140703No Hit
CAAGGAGGAGGCAGAAGGAGACTAGTAGGACCAAGATGCAGGTGCTGCTG10.5025125628140703No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCAC10.5025125628140703No Hit
GGCGCTGCCCATGGGAGCCCTGCCCCAGGGGCCCATGCAGAATGCCACCA10.5025125628140703No Hit
GCCCTGCAGCCTCTGCCTCCCAGGCCCAAGCCACTCCCGTGTCCCGGCCT10.5025125628140703No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCC10.5025125628140703No Hit
CAGAGGGGCGAGATGCAGGCTCAGCACCCTCCGGAGGGGACCCGGCCTTC10.5025125628140703No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT10.5025125628140703No Hit
GCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACG10.5025125628140703No Hit
GGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAGAACC10.5025125628140703No Hit
CTTTCTCCCCTCCCCCACCCGGCACCTGGTGGCACCTGTCTCTTATACAC10.5025125628140703No Hit
CTTCCCAAATGGCCAAAGTGTCGGGGAGAAGATTTTCAAGACAGCAGGCT10.5025125628140703No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10.5025125628140703No Hit
ATCTTCTTGATAAGCTTCACTTCCTCATCTAGGAAGTGAGTCTCCAGGAA10.5025125628140703No Hit
ATCTGGAAGTTGTGGACTTTTCTAGTGAGAGCCTCAAGACTGGGGATGCT10.5025125628140703No Hit
ACGCAGAGTACATGGGCAACGCAGAGTACATGGGCTGTCTCTTATACACA10.5025125628140703No Hit
AGTCTATGGTCTCCATGGTGTTCTTAAGGTGTCTCAGGTTCTCCGGGAAG10.5025125628140703No Hit
CACAGGGTGCTCACGGCCATGCCCAGGAAGGACGGGTCCCCTCCGGAGGG10.5025125628140703No Hit
GTATAGTATCTCTCTAACACACAGAGAGCATTTCAAAGGGTGGCTATGGC10.5025125628140703No Hit
CTGCCACGTGGTTCTTGCCGATGACCTGGTACTTTACGTAGGATTTCCCA10.5025125628140703No Hit
CCCTTATATTCTCTCCTGCTAAATCATGGAGGTTTGGGGAGTTCCCTCAG10.5025125628140703No Hit
CCCGACCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATT10.5025125628140703No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG10.5025125628140703No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC10.5025125628140703No Hit
GAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCTGTCTCT10.5025125628140703No Hit
CTTCAACTGACAGAAACCTTCACTTGAAAACAATGATTTTAATATATCTC10.5025125628140703No Hit
GAGCTGAGATCACGCCACTGCACTCCAGCCTGGGTGATTGAGCACGACTG10.5025125628140703No Hit
CGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGT10.5025125628140703No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT10.5025125628140703No Hit
GTCCGGCGGGCTCTCCATCAGGACCTCTTTGCTGCCCACATCCATCTTGC10.5025125628140703No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA10.5025125628140703No Hit
GTACATGGGGGGAAGCGGTTCAGGAAGTGGAAGGCCCTGAACCGGAAGCG10.5025125628140703No Hit
CTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTTCCT10.5025125628140703No Hit
TTGCAAGAGAATAGGCTATTTTGTGGTTTCTTCCTCTTCTGAACATCATG10.5025125628140703No Hit
GCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCT10.5025125628140703No Hit
GTACTGCACCGGCGAGAGCCATCTGGTCCCCCTTAAACATTTTAAGCTGG10.5025125628140703No Hit
GTGGGAGACCACGTTTTATTCAGTCCAGTTCAGGATCCCCGCTATCTCAG10.5025125628140703No Hit
CCCTGTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGTCTG10.5025125628140703No Hit
GGATTTTCAGGGCAGCTGCCAACTTTGGCTTTCTCTGTCTCATCCCTGTC10.5025125628140703No Hit
GGTCATGTGTAGCTCAGTGGAGAGTGTCCTGCCTGGTCGCGATGGACGGG10.5025125628140703No Hit
GCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGC10.5025125628140703No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG10.5025125628140703No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA10.5025125628140703No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG10.5025125628140703No Hit
GGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCC10.5025125628140703No Hit
GTGTTTCATCCTGCAAAGCTGGGGCAGAAGGTTTTTCCTTGAATGTGGTC10.5025125628140703No Hit
GCCGTGAGCACCCTGTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGC10.5025125628140703No Hit
GGTATGGCCTGCTCGCTGCTGTTCCCGGCCTGGGTGGTCACGGACATGCA10.5025125628140703No Hit
ATTCAGCACCATGCGGAGCTCTTCATAGTTGATACAACCATTGCTGTCCT10.5025125628140703No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC10.5025125628140703No Hit
ACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGG10.5025125628140703No Hit
CTGACCATCTGTGAGTTCTGAGAGGCATTTTAGGCCATGGGACAGGGAGG10.5025125628140703No Hit
TCTACCAGGGGCTCGTGCACAAAGGTGTTCACTGGTTTGCCTGTCTCTTA10.5025125628140703No Hit
GTCAAGTTCCCGGGGGCCGTGGACGGCGCAACCTATATCCCTGTCTCTTA10.5025125628140703No Hit
GAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCG10.5025125628140703No Hit
TTTCCTGGCAGCTACATTCCTGCTCCTGGCGCTCAGCACCGCTGCCCAGG10.5025125628140703No Hit
CATGCAGGGCCCGGATCTCCTCAGTGGGTCCTGTGATGAGGGACGTGCCC10.5025125628140703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5025125628140703No Hit
ACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTAG10.5025125628140703No Hit
GGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAACTGCGTTTA10.5025125628140703No Hit
TGCCTGGACAAGCTGGCCCAGTTGGGTCCAAAGGGGACAATGGCTCTGTT10.5025125628140703No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT10.5025125628140703No Hit
GAGTCAGGGCCCATCAGGGGAATGAAGTGGCTAAGGGTGCCTCCAGCTCT10.5025125628140703No Hit
CACCAGACCCAAAACCCGCTTTTCTTCACGCTCCTGTCTCTTATACACAT10.5025125628140703No Hit
TCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTG10.5025125628140703No Hit
ACTGAAAGTTTCTCGGCAGTAAAGTTTATAACATCTTTAGATTTCTCTTC10.5025125628140703No Hit
CAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAA10.5025125628140703No Hit
CGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGC10.5025125628140703No Hit
CCACTGGGGTCAGTCACTCGAAGCACAATAAATATAAAATGTGGTCCTTC10.5025125628140703No Hit
ACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGG10.5025125628140703No Hit
ATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATG10.5025125628140703No Hit
GCGTTTCAGGTGCACTGGGCAGCAGGGAATGCCAAATCGGCCCCGGGGAG10.5025125628140703No Hit
ATATCTGGGTGAATGGATTAATGAAAGGAGAAAGCAGAGCCAGTGGTGTG10.5025125628140703No Hit
CTGTATGCACCTGGGCCTGTGCAAATCCCGGCAGCCAGAGCCAGAGCAGG10.5025125628140703No Hit
ACCTGAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAGCGG10.5025125628140703No Hit
CCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTG10.5025125628140703No Hit
GCTGGGGCGTGTGCTGCTCCACAAATTGCTTGCACTGAGGAAGGAGACAC10.5025125628140703No Hit
CCTCCGCGCTCGGCGCCAGGCCCTGGGTCCTCAGACGCATCACTGTCATC10.5025125628140703No Hit
GCAATTTCTGGCTGGTTGGTGATCACAGCCAAGTGGAGTGGAGTCTGCTG10.5025125628140703No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG10.5025125628140703No Hit
CATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCC10.5025125628140703No Hit
CCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCAGGGCTCCCACAATC10.5025125628140703No Hit
GGGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAAT10.5025125628140703No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG10.5025125628140703No Hit
GCAAGACTGAGGACTTGGCACCTCACAGTCCCTGTCTCTTATACACATCT10.5025125628140703No Hit
TTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATT10.5025125628140703No Hit
GTCCCGCAGAGGTTTGGGCAGGGGGTCTGACATCCCTGGCTCCTGCTCTG10.5025125628140703No Hit
CGATGACCAGGGGGAAGTAGTCGTCAAGCACTTGGTTGCACTGGGGCATG10.5025125628140703No Hit
GTGAAGAAGCATCTGCAGGACCTGTCCGGCAGGATCTCCAGAGCTCGGCA10.5025125628140703No Hit
AGCGTAGGCTACCGCCACATTGATTGTGCTGCTATCTCTGTCTCTTATAC10.5025125628140703No Hit
CCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCACTGTCTCTT10.5025125628140703No Hit
CGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAGAG10.5025125628140703No Hit
CGAATTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCTGCTTATCGT10.5025125628140703No Hit
CTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGG10.5025125628140703No Hit
GGTCATCGGCTCCCACATGTCCCCAGACTTCCTGTAGGCAATGCCCTAGG10.5025125628140703No Hit
ATCGGGGGTATGCTGTTCGAATTCATAAGAACAGGGAGGTTAGAAGTAGG10.5025125628140703No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC10.5025125628140703No Hit
ACTAATGACAAGGGCCGTTTGAGCAAGGAAGACATTGAACGTATGGTCCA10.5025125628140703No Hit
GAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGG10.5025125628140703No Hit
CCGCCCGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAAC10.5025125628140703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.5025125628140703No Hit
CCGGGGCCCTCCAGGCGAGGCCTGGGCCTCACACACAGGATCTCTCCGAG10.5025125628140703No Hit
GATTAGAGTGTTCAATTACAATACTCTGGAGAGAGTTCATATGTTTGAAG10.5025125628140703No Hit
GGCCTCCAGGGCACACCAGATCTTTGAGGAGTTCATTTGCAGTGAGGCCC10.5025125628140703No Hit
GACCTGAAGACAGCCAGCCTAGGCCTCTAGGGTGACCTAGAGCCGCCTTC10.5025125628140703No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA10.5025125628140703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)