FastQCFastQC Report
Tue 10 May 2022
EGAF00003629447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827505
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT42630.515163050374318No Hit
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA38880.4698461036489206No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG32640.39443870429785927No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG29570.3573392305786672No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC26100.31540594920876613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22680.27407689379520367No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC22420.27093491882224274No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG21800.26344251696364374No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG20940.25304983051461927No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT20180.24386559597827204No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA20110.24301967963939794No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG19520.2358898133546021No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC18880.22815572111346757No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT18430.2227176875064199No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC18060.21824641542951403No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA17760.2146210596914822No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG16770.2026573857559773No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG16570.20024048193062277No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA16160.195285829088646No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC15200.18368469072694427No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT14930.18042187056271564No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA14610.1765548244421484No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC14030.16954580334862024No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA13990.16906242258354934No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT13840.16724974471453344No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA13750.1661621379931239No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT13720.16579960241932074No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT13520.1633826985939662No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC13020.15734043903057987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.15709874864804443No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC12950.1564945226917058No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC12790.15456099963142217No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC12580.1520232506147999No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC12450.15045226312831947No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT12160.1469477525815554No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG11700.14138887378324No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG11560.1396970411054918No Hit
ACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGG11470.13860943438408227No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG11370.137400982471405No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT11310.13667591132379864No Hit
CCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAACTATCTTCCTG11290.1364342209412632No Hit
CCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTG11240.13582999498492457No Hit
CCATCAGCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTG11030.1332922459683023No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA10850.13111703252548323No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT10770.1301502709953414No Hit
GAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGAT10720.12954604503900277No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG10540.1273708315961837No Hit
TATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAAT10500.1268874508311128No Hit
CACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCT10460.1264040700660419No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC10400.12567899891843554No Hit
GGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTA10310.124591392197026No Hit
CGTTTATTGAGAGGGGCCGGGGGAAGGGGATGGACGGTCCTCCCCGCGGC10280.12422885662322282No Hit
AAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGG9760.11794490667730105No Hit
GGTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACT9570.11564884804321424No Hit
GGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGG9390.11347363460039517No Hit
CCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAA9300.11238602787898562No Hit
GGGGAAGGGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCG9110.11008996924489882No Hit
CTACAAAGGCTGATGGTCACCAGCAGGAAGATAGTTACCAGCTTCATGAC9000.10876067214095383No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA8990.10863982694968609No Hit
GTACATGGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATT8710.10525616159418975No Hit
GTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAAC8650.1045310904465834No Hit
CTTCCATCCCCATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGT8560.10344348372517387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.10344348372517387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)