FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003629491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAGCTCGGCGGCGCGCTTCGTGCCAGACGCCAGAGGAAGCAGATAGC312.0No Hit
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA28.0Clontech Universal Primer Mix Long (96% over 26bp)
CGCCAGCATCACGCGCCGCCGCCGTGCCAGGCGGTGCCCAGGCGGCAGGA28.0No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28.0Clontech Universal Primer Mix Long (96% over 26bp)
GGGCGGAATGCGTGGCGGTGACGTCGCCCACCGCCTCGCCCGCCTCGATG14.0No Hit
GGCCAGGACCGGCCCCGCCGCCGCGCCGAAGGAATAGGCCAGCACGAGGC14.0No Hit
GAATGGAAATAAACCTTTCTGTATCTGCAGCCATGAGCTGTCTCTAGAGC14.0No Hit
TTATCAGTCTGCATAAGAGGGTATTCAAACTTTGGAGAAGGCTATTTCAA14.0No Hit
GCATATATATATATATATATGCACATATGTTCATGAAAACGCATATTGGG14.0No Hit
GGCTTCAATCTACTTCTCCCTCCATGTGGGGGGAGGGTGGTAGGCCGGAG14.0No Hit
TCCTGCGCCTGCTTCGGGGGGAAGACCTGGAGATAGTGTCGCGCTCGCTC14.0No Hit
CGGGCGATGGATGTCGCGCCGCGCTGATCGGCCGGGACACCATCCGGCCT14.0No Hit
ATCCTGGCCGGTGGCGTGCTGGCAAAGCGCGGCTCGTTAGCGAGTTCGAC14.0No Hit
GCAGCAGGATGTGGAGCCGGCGATAGCCCTGTCTCTTATACACATCTCCG14.0No Hit
GGATCCATTCGCGCGTGTCGTGGATGTTGCGCCCGGTGGAAAGGTCCATG14.0No Hit
CTAAATGCCCACAAGAGAAAGCAGGAAAGATCCAAAATTGACACCCTAAC14.0No Hit
CTCCCAGGGGAATGAAGCCTAGTGAACAGGCTGAGGCCTTTCACAATGCC14.0No Hit
CAGTGGTATCAACGCAGAGTACATGGGAATATATGGCGACCCGAGTTACA14.0Clontech SMARTer II A Oligonucleotide (100% over 22bp)
TCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATG14.0No Hit
CTTTTGTCTGAGCCTGAGGGCTTCTCCAAAGGTTATTATCTGCATTAGAT14.0No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)