FastQCFastQC Report
Fri 10 May 2024
EGAF00003629492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Total Bases2.5 kbp
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGACGTCTCCATCAACCATGATTCCAGTGAGGGCCTCGGCTATCTC312.0No Hit
GGATGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC28.0Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT28.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACGGCACTGGCCGGTGCGGCCGAGCTGTCGCTCTCGGCCGCACCGGCC28.0No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT14.0No Hit
TTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCCTGTCGATACCAGA14.0Illumina Single End PCR Primer 1 (100% over 26bp)
GGCTATCGCCGGCTCCACATCCTGCTGCCTGTCTCTTATACACATCTGAC14.0No Hit
CTATTAAGCTTATACAAAGTTTCTCGTTTGAATGTATAAATGATTCATGA14.0No Hit
GGTATCAACTGTAGAGAAAACGTCTGTGAGGAGTTTTATGGAGGAAGACT14.0No Hit
CAGTTTGGTTTTAAGAACTGTCACCTCTCACTTAAACTTTGCTTGTTCCT14.0No Hit
TCTTTGCAGCATAAATACAAAAACATTTAATTTAAGCTGATTTCCTTTAC14.0No Hit
GGTCCGGGCTGATCGCCGGCCCACGGCAATCTGGCCCCGGCCCCAACATG14.0No Hit
GCGTCGGACTCAACCTCCTGCGCAACGGCCTGCTCGCCACGCTGCGCGAG14.0No Hit
GTCTATTGATTCTTCTCTGTTTTCTACTGTATTAGTCTTGCTAGCGGTCT14.0No Hit
AAGATGGTCTGGGCGATCCGCTGGGGCGCCGACACCATCATGGACCTTTC14.0No Hit
GCCAGCGCCTCCGGCCCCGCCGCCACGGCGCGGATCTCCGGCCCTGTGAG14.0No Hit
GAATATAACAATTTAAAAAGCAATGTAAAATTATATGGCAAAGACAGTTT14.0No Hit
GAACAGCAGGTATAGTGTTCCGATAGCTTTGTGATTGGTTGAAAACAATC14.0No Hit
CTGGTGCTCCTGGCTGCTCCGCACGGCACCGCCTGGCTGCTGACAACGGG14.0No Hit
CGGCAGACCCGGGCCGTGCCATACGGCCTGCCGCTGCTGGGCGAGACGGC14.0No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)