FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length100
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGCACGAAGCGTGCGGTCAGGATGTCGATGTGCTGCTTGTCGAGCG38.333333333333332No Hit
CACCGCCACCGCGTAAGCGTTCTCACTCGGCACCCCCAACGACCTTGCAT38.333333333333332No Hit
GCGTCAGTCAACCTGCTTGCCAACGAGACTGAACGAGACTGCAAAACGCA25.555555555555555No Hit
GCCCTGGTGCTGTGCTTCAGCATCGGCCTGGCCGCGACCATGGTCGCGTC25.555555555555555No Hit
TTCAACAACCGCACGAGCCCGCGCTGCGCGCCATCATGCTGAGCCTGGAA12.7777777777777777No Hit
GGCCTGGAATGCGGCGGGCGCGAACTGGCCCAGAGCGATACCCGCGATGA12.7777777777777777No Hit
GCCGTGGTCGCCGCCGCCGGCCGGCGCCTCGTCATCGACGCGATGAGCAG12.7777777777777777No Hit
GCACTGGGCCCGGTCGGCAAGCCGGACCGCATCGCCTCGCCGCTGCAGAT12.7777777777777777No Hit
CTGGAAGGCGGCACGGCCGGCCTGCGCGTGCCCACCGGCGAGGTGCGCGA12.7777777777777777No Hit
CAATCGCGCCAGTCATCCCCATCCTGACGGCGGCGACCAATGACACGCAG12.7777777777777777No Hit
GCTGATGCTGCTTGCGGCCGCGGTGCAGGCGTACTGGGGGACGGCCGCTG12.7777777777777777No Hit
GTTCATGACCCTCAGGGAGCATGACGCCGCGGACTTCCGCTATGGCGCGT12.7777777777777777No Hit
ATCCGCAAGCAGCCGCTGCTGGGCATGGAGCGCGCACAGATGCAGCCCGA12.7777777777777777No Hit
CCGCAGGCCGGGATCACGGGCGCTGATGCCCGGCGGGGCGCGGCCTCAGC12.7777777777777777No Hit
GGCTGCGCGCGCTGGCGACGCCCCTGCCCGGAGTTCGCCGACGGGCTGCA12.7777777777777777No Hit
ATGACGTCCTTGACCATGTGCTCGTTGTCCATGAAGTAGGCCGGACGCAG12.7777777777777777No Hit
GTACTGCGGCGGGAACCAGCCCGAGCCCAGGCTCAGCGCCACGCCGAAGC12.7777777777777777No Hit
ATCCGTCATCGGACCGTGCTGACCACCCAAAGGCGGCTCTCGTGGAGATA12.7777777777777777No Hit
GTCCTCGCCGAAGGTGCCCATGGCGCGTCCCTTGTCGTATGCGTCTTCCA12.7777777777777777No Hit
TGCTCACCCAGTCCTCGTAGCCGAGCACGGCGAAGCCGCTCGCGCCCCAG12.7777777777777777No Hit
GCTTATCCTGCACCAGCAGACCCGCCAGCGCCTCCTCCAGGTTCGGAAAG12.7777777777777777No Hit
GATCAGGTTGAACTTGCACGGCACGTCGGCCACGACGCGCAGCAGTTCAC12.7777777777777777No Hit
CGATGGGGTGGGTGCCGGGCTTGATCTCGAAGCCGGCGGCCTGGATGGCT12.7777777777777777No Hit
ATCTTGTCGTTGATCGCCCAGAACGCGAACTGCACGGCCTGCAGCGTGAA12.7777777777777777No Hit
GGTCCAGCGCCTCGACCTGGATGTCGATCGGATAGACCGAATCGCTGCGC12.7777777777777777No Hit
CTCCAAGCCGGGGTCGCGGCACGTGACCGAGTCCCCGCCGAGGGCCTCTC12.7777777777777777No Hit
GACCCATGACGATGCCGACCAGCGCGAACGCCGCGACCAGCCCGTTGTGC12.7777777777777777No Hit
GTCGTCCAGGTGCTTGGCAGCACCGGTCAGGCTGCGAATGAGGCCCTTCT12.7777777777777777No Hit
GACCAAACACGGCGAGCGTTGCGCAGTGGTGGGTACGCTCGGAACCGGGT12.7777777777777777No Hit
ATCCGTCATCGGACCGTGCTGACCACCCAAAGGCGGCTATCGTGGAGATA12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)