FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGCACCGGCGACCTGACAGCGAAATTCTCCCGCCTGGTCGGAGAAA42.13903743315508No Hit
CGGCGGTGCCGGGCGCGCCGGGCACCAAGCTCGCAGGCTTTGTCGCCAAG42.13903743315508No Hit
ACCCCGTCTCCACCAAAAAAATACGAAAACCAGTCAGGCGTGGTGGCGCA31.6042780748663104No Hit
GCGTCGTGGTGCCGCAGGGCCAGATGCGCGAGGCCTTCGCCGAGCTGCTG31.6042780748663104No Hit
GTGCATCATCGCGCGCTGGATCACGCGCTGGAATCCGAGCGTCGGCTGCG31.6042780748663104No Hit
GGTCGAGCCCGAAGTGGTGCACGCCACCGCCGAGGTCGGCGACGATCGTG31.6042780748663104No Hit
ACGCTGGAGCAGGCTGTGGCCAAGGAGCCGGCCAACCCCGAGATCGTGGA31.6042780748663104No Hit
GGCGGCACGGCCAGCGGATCGAACTTCACGATCAATGCGACCACCGCCGG31.6042780748663104No Hit
GCGAAATATGCTTACCTACTGGCAGGCTATTGAATATACGAGTAGTCAGA31.6042780748663104No Hit
GCTCGGACCAGGTCGGCGATCAGCTCCACCGCCAGTGCCACGTCGGCCGG31.6042780748663104No Hit
GTGTTCAACGTTAAGAAAGATGGTTATACGACTACTTACCGCGACCTCTT31.6042780748663104No Hit
CAACAGGGACAAGGATTTCATAGCAGGACGACGACACAAGGATTTCATAG21.06951871657754No Hit
CTGCCCGCCTGGCCGGCGGCGCCGGGCATTCACTGAGGTGATAACGCACA21.06951871657754No Hit
GACCTTGACCGGGATGCCCGAGTTGGCAATGTCGGTCGCGGAGCGGTGGC21.06951871657754No Hit
GCTAGTATGAGCACACACCATCACGCTTGGCTATATATATTTTTTTTCTA21.06951871657754No Hit
GGACCAGGCGGTTGTCGCCCTGAACCAAAAGCTGGACGGCGGCGGCATCG21.06951871657754No Hit
CTGTAGTACGGAAGATCGAAGCCCAGCACCGACACGGCCGCGCTCTTCAG21.06951871657754No Hit
CCTTCCGGTTGCCGCCTTGGATCAGGCTGAACGTGCCCTTGGGCATGCCC21.06951871657754No Hit
CATTTCCTCAGATGTTTTCTTGAAAATTCTTTCCTCAAGAAAGAATTAAG21.06951871657754No Hit
ATTCTGAACATTATCGCGAGCCCATTTATACCTGAATATGGCTCATAACA21.06951871657754No Hit
TCCCCGACGGCGTAGGCAGCCTGTGCTGCGGGACGCCGTGGTCGTCCAAG21.06951871657754No Hit
GTTTCAGAAGGTACACAGAGGCTGTTTACTGAGCAGAATGCCTGGAGAGC21.06951871657754No Hit
GATCCGGGCTGCGGCGTCGTCGACAAAGCTGCCCAGATCACGACCGACAA21.06951871657754No Hit
CACTTAGGGCGACTTTGCTCATTTGCATGTCCCCGCCCAGACACGCCCAC21.06951871657754No Hit
GTTCTTGGGGTTTTTTAGCTTTTCTTTTAAATCTTCCATCTCTTGACCTT21.06951871657754No Hit
CGTCACCAGAGTCGTCATAGTTTCAACGTGAGAGTCAAAGGTGATAGCCA21.06951871657754No Hit
CTCCACCATTTCTGGCAGCATTTCTGCAGGTTTGGTGGCGACCGACCGCG21.06951871657754No Hit
AGCTTGGTGGTGCCAGCCGTGCCAGCGGAAGGTTGAGGTCGCGGCATGCT21.06951871657754No Hit
TAATTGGAAGGGCGCTACTAGCGGTTGAGTATGGCAACGAGCGGGGCGGT21.06951871657754No Hit
CCAATGGAATAGAATAGAGTTGTTAAACAGATACTATTTAGTGCAGGTTA21.06951871657754No Hit
GACGACACCCGTCGAGGCGCACGGGATCTGGACCGTCCGGTTCCAGCAGA21.06951871657754No Hit
CTTTCGCTCCAGGGCTCCGAGATCGTACGCACCGTCGGCGACCCGGTCCT21.06951871657754No Hit
GGCCAAGGCGGTGTCGACCTGGCCGAGCGCGGCGCGGAGCGCGTCGAGTT21.06951871657754No Hit
ATCCTCGGCGGCGCGCGCGATCGCCTGCTGCACGTAGAAGATCGAACCGA21.06951871657754No Hit
TTCGTTGACCGAGCTCCACGCCGCGCGCCTGTTGACGTACCACGCGGCGC21.06951871657754No Hit
ATGGAATGGAATGGAAAGGAATAGAATGGAATCAACCTGAGTGGAATGGA10.53475935828877No Hit
GTTATACATGACAAGGAGCAAAGCATGAACTGCAAAGACAACTCCCCAGG10.53475935828877No Hit
GGCCGAGGCGCCGGGGGTGCGGCTGACGCGGATGTCCGGCTCCGGCGCCA10.53475935828877No Hit
GTACCAGGCTCTGAGGATATAGCTGTCTGCATAGGGCTTCCTGCTTTGTA10.53475935828877No Hit
CGTTTCTTTCATGCGCGAGTCCTGCTCGCGCATGCGCTCGATGGCGACGG10.53475935828877No Hit
ACCACTTGACAAAGGTGGTATTTTCCAGGCTTCTGCACTGCAAAGTTACT10.53475935828877No Hit
CACCTCATCAATGCATCTTTTCAAACACCTCATCAATGCATCGTTTCAGG10.53475935828877No Hit
GTTCTCCGCGAAATCTATTTAGGTAGAGCGTCCGACGAATTCCTTGGGGG10.53475935828877No Hit
GCTCTCGATGGGCCGGCGTTGGACGCCCTCGCTTCGAAACTGGCCCTGTC10.53475935828877No Hit
GGATTCGAGGGTGGTTCCGATCGATGGTTGCGCGCAAGCAATCGGCAGGC10.53475935828877No Hit
GGAATGGAATGGAACGGAACGGAACGGAATGGAATGGAATGGAATGGAAT10.53475935828877No Hit
GCACCGGTGATATGGGCTTCCCTTCGGCGAAAACCTACGACCTCGAAGTC10.53475935828877No Hit
TTACAAACAGAGTGTTTCCTAACTGCTCTATGAAAAGAAAGGTTAAACTC10.53475935828877No Hit
GTCTCCGTATCGCTCGACGCAGTGAAATCCTGCGATCAGACGATTCTGAT10.53475935828877No Hit
CCACAGAGTGGGAGCGGGCTCAAGCTGGCTGCTCAAGAGAATTTTCTGGG10.53475935828877No Hit
ACTCCAGCTATTCCGCCAGCAAGGCCGCGCTGCTGGATGCCTGGGTGTAT10.53475935828877No Hit
ATCCAGGCTCACCACAACCTCCACCTCCCAGGTTCAAGCGATTCTCCTGC10.53475935828877No Hit
GTCCGGTGCCCGCCGGGAGCGCGATCACCGTCGCCGCGACCGTGACCACG10.53475935828877No Hit
ACATCCAGGCTTTTCTCAACGAGAACAATGCGCTCGGCGCTGCGGTCCAC10.53475935828877No Hit
GAGCAATGCACGTCGCCCCGGCGGCGCGGGTGGAGACGACCAACACCCAC10.53475935828877No Hit
CGCGGTGGCGATGGCGTCGACCTCGCCGGGCGGCATGCCGAGCGCGGCGC10.53475935828877No Hit
CTCCAGCAGCGCCTTGCGGTGGCGCGCATAGCCGGCATGGGCCAAAGGTG10.53475935828877No Hit
TCGCTGGCGCCGGCACGCTCGGCAGCAGCAGCGCCGCCACCACTGTTGCG10.53475935828877No Hit
GTATCGGGCTTCGGCGTAGTCCAGCAAAGTTCGGTGCTGGAGTCATGGTG10.53475935828877No Hit
GCCGGGGCGTGGCGGCGCAGGATCGGCGCGCAGACCCGCACGTTGGCGTC10.53475935828877No Hit
GATGATCACAATGGATTTCATTCCATCATTCTATTCGATTCCATTTGATG10.53475935828877No Hit
GAACTGGTGTTCGCCATCTGCGAGGCAAAGAATGTCGAGGTGGTGATTCT10.53475935828877No Hit
GGGCGAAATCACTGGTGGAGAGCATTCAATGCGGCAAGCTCGAAGATCTC10.53475935828877No Hit
ACAACGACGAGGCAAACAAGGCCGATCCGCGCTTCGTCATCAACGCCCAG10.53475935828877No Hit
GCTTTTTCATTCCCCCTTCTTGTGAACAGTCCACTTTTATTCTAGTTCTG10.53475935828877No Hit
CTCTTGTCCCTCCCTGATTAGCTTAATAACTAATTTCCTGAACTCTTTTT10.53475935828877No Hit
GTTCATATCCTTCGCCCGCTTGTTGATGGGGTTGTTTGTTTTTTTTCTTG10.53475935828877No Hit
TGCTCAACGTACGCGCTATGCCGCAGGTCAAAACCGACCTGGCGGTATTC10.53475935828877No Hit
CTCCAGCACGCCGTCGAAGATGAGCGTGGCCAGGGTCTGCGCCGGCGGGC10.53475935828877No Hit
AATGAAAGGAGATGTTTACCTTGATAATAAGCCCAGCTGACTTGATTTTT10.53475935828877No Hit
CGTCAGGTCAGTTGGCAGACTGTGCGCGGGCCGAACGGACGCCCGCCGGC10.53475935828877No Hit
GTTTACTTGGGGGTACTTAGCATAGGATAGTCAGGAAGACTTTTCTGCAT10.53475935828877No Hit
GCGTCAAGAAGGCGGCCTACCTCGCCTTTGGGGTCGCCGCGCTGGCCGGC10.53475935828877No Hit
GTGTACGTGGACACCCAGGCGTCGACCTGCCAGCGCGGGGCGCCGTAGAC10.53475935828877No Hit
ACGATGGGGGAAGTCTCCCGAAGAGAACTCCCCTCATCGACCCTGGATCG10.53475935828877No Hit
CTATGTTGCCCAGGATGATCTTGAAATCCGGGCCTCAAGTGATCCTCCTG10.53475935828877No Hit
GTCACTGGACGGTTCGTCGCGATGATGCCGCCGAACGCGCTCGTCGAATC10.53475935828877No Hit
GTCCTTCCGGTGTCTGTCGTCATGCCAGGCGGTGTGCTCCGGCATCGGGC10.53475935828877No Hit
TAATACGGCTTGGCTACTAGGCTGCTGGCTCTGCTTCCTTCTATAGATAG10.53475935828877No Hit
TCACACCTGTAATCCCAGCACTTTGGGAGACCCAGGCGGGTGGATCATGA10.53475935828877No Hit
CCCTGATGGTGTGCGCCCAGGTGGTCGGCAACGACGCGGCCGTGGCCTGG10.53475935828877No Hit
GGTCCGGCCCGGTTGGCGATGGCGCGGTTGATGGCGGTGGCCGTGTCGGT10.53475935828877No Hit
GCCTCATGCTCCTGGACGGCTTGATTGCCGCAGCCGTCTTCATCGGCGTG10.53475935828877No Hit
GCCCATGACCGGCGGCGCGATCCAGCCCGCGAACTGGCCCGGCTCCACGC10.53475935828877No Hit
CCATTTATTATAAGACCATGTTTCAGCCTTGTATTTTTCCTTCAGGCTCA10.53475935828877No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACCTAATACATCGCGTATG10.53475935828877No Hit
CTTGGGAGGCTGAGGCAGGAGACTCGCTTGAACCCGGGAGACCGAGGTTG10.53475935828877No Hit
GGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCC10.53475935828877No Hit
CAACGCCGCCTCGGCGCAGAAGCCGCGGGCGGTGATCGACGCCATGGTGG10.53475935828877No Hit
GCGACGCGTCGGGGACCGGGTACTGGTCGCCGGCCGAGGGACGGTGGCGA10.53475935828877No Hit
GAACAGCTGTTAGTCCCAGTAGAGCAGTGCTTAGATTGGAATGTCTCTGG10.53475935828877No Hit
GGACCGTGACGGTGCGCGACGCCGACAACACCGTGATCGAGGGCTGCGTC10.53475935828877No Hit
GTCATCCATCGGCCGAAGCTGCGCGGTACAGATTTCTGTGACGAGTGGTG10.53475935828877No Hit
CTCGTTGGCTGGCTCGACTTCCGCGAGCGGCACTGCTGGACGATCGTGAC10.53475935828877No Hit
GGGCGTCGGTGGTGACGCCGTCCCGCCGCATCTCGTGCAGCCGCACCGGC10.53475935828877No Hit
GCATCGGTCACGACCGGATGCTCGCGCGCAACCGGCCCGCACCCCCTGCC10.53475935828877No Hit
CATCGAGGGCGGCCCGATCGGCGACGCGGCAGCGCAGGCGGCGGCGGCCG10.53475935828877No Hit
GTTCAATAGTGTCTCTGACCTGCTTCGCTCCCTCTATGAAGGGAGGTCGC10.53475935828877No Hit
GTATTGGGCGTGCCGAGCGGTGCGAACGTGTCCAGTGCGACCTGCACGCC10.53475935828877No Hit
ATGGAGCACTGACAAGAAGATTTCCGAAGTCGTCGCCGAGAAGAAGAACA10.53475935828877No Hit
GTCCGGCGCCCGCCGGGAGCGCGATCACCGTCGCCGCGACCGTGACCACG10.53475935828877No Hit
GTCTAGTATGCAGCTGCTGCTACCTTCTCCTACCTGCCTGCTGTGCTGGA10.53475935828877No Hit
ACTGCACCCGACCTTTTTTTTTTTTTGAGACAGAGTTTGGCTCTTGCTGC10.53475935828877No Hit
ACAGATTCCGTGCAATCTCTCCCATAATACCAATGTCATTTTTTTCACAG10.53475935828877No Hit
GTAGAGACAGGGTTTCACTGTGTTAGCCAGGATGGTCTCAATCTCCTGAC10.53475935828877No Hit
CCGATGAACCGCGTCGCCACCATCCCGCTCCAGCGCACCATGTCCGACGC10.53475935828877No Hit
GTTCTTCAGATCGCGTTCGATCGTGGCGAGCTTGCTGCGGTCGTCGGACG10.53475935828877No Hit
ATCCATGACCAGCTGGCGGAGGGCTGGCAGTCCCTACTGAAGGGTAACTG10.53475935828877No Hit
GTAGTGACTCCATCTTGGCTCACTGAAACCTCTACCTTCCAGGTTCAAGT10.53475935828877No Hit
CTCTTTTACTGCATTTCATGAGTGACCCTGACAACAGAAGGACGTTGTAA10.53475935828877No Hit
ACCCTCCTGCTCGTGGGCGACGGCCCGGAGCGGGCGCGCATCCGCAACCT10.53475935828877No Hit
GGACCGGGCCGGCATCCGGGCGGCCATCCGCAGCCGGGTGATCCGCACGT10.53475935828877No Hit
GAAAAAGCTTTGGCATAGGCCTCTTGGACAACATCTTCGGCGTCAGCCGG10.53475935828877No Hit
AAGTGTTGGGTTTATAGGCATGAGCCACCACGCCCAGCCAGCATTCAAAA10.53475935828877No Hit
TTCTTGGACTTTTTGGAGAAAGATATGCTGGACCCAACTGGGGAGCCCTC10.53475935828877No Hit
CTAATAGTGCTTCTGTCTACCTTTTGATGATGATATTCCCGTTTCCAACG10.53475935828877No Hit
GCACAACATCGCGCGCGGCCCGTCCAAGGGCGGCATTCGCTACCACCCGG10.53475935828877No Hit
CTTCATGACTTTTTTTTTTTTTTTTTTAGACGGAGTCAAGCTCTGTCGCC10.53475935828877No Hit
GCTGAGAGCGGTGCTTGAAAACCGCAGTTAGTCGCCACAGGCTTCGCTGC10.53475935828877No Hit
CTTCATGACTTTTTTTTTTTTTTTTGAGACGGAGTCTCGCGCTGTCGCCC10.53475935828877No Hit
GCCTGGTATCGCGATCGAGTTGACCGCGCGCGCGTCGATCTCGTCGAGGT10.53475935828877No Hit
CTCCGGCTGTTGATCCCAAGATCGGTCAGGCTGCTCCGGCAGTGACCGAG10.53475935828877No Hit
GATTATCTTCGCGCCGTATCAATTCAACAACCAATCAGCATCTCACACCG10.53475935828877No Hit
TAAAAGTACAGGCCACCAGCCAACAGGCCCCTTCTCCCAGTGGCTAGCAT10.53475935828877No Hit
GCAGCGAGCTGATGCTTTTTGCTGTCGCCGCTGGCTTCTCGCTGTCGTTC10.53475935828877No Hit
CACGATGCACCGCCTTCGGAAGGGCGGCATCAAAGAACACCGCATCACCA10.53475935828877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGTAATA10.53475935828877No Hit
GATAAGGCTGCCTTGGCTGGTTTTCAAGCGGATGTCTGGGTGGATTTTAC10.53475935828877No Hit
TGTCGAGGCTGGGTTCGTCGAGCTCATCACCTTGCCATTCATCGGGGATA10.53475935828877No Hit
TGTGGGGACTGGGCAACGCGCTCTTCGTGGCGACCGCGCTCGCGACCATC10.53475935828877No Hit
GGCTCGTGAACGGCGTTCAGCCAGATCTCGAAGTTCAGCGGCGTCGGCCA10.53475935828877No Hit
GTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATC10.53475935828877No Hit
GTCCCAAACACGAGGTATTGCAATGATTTTCTCTAGGATATCTAGCTGGG10.53475935828877No Hit
TGGCGAATCCTCAGCGGGCGCCGGTTGTAGTCCCAATAGGTGGTGCGGGT10.53475935828877No Hit
CAATCATTCTATATCTGCGCAGCCTGAAGCCATTACCTCTGCCCGACTGA10.53475935828877No Hit
GGTTAACACGGTGAAACCCCGTCTCTGCTAAAAATACAAAAAAAATTAGC10.53475935828877No Hit
GTCAATGGGTTTCCTTGCGAAAAAGCCGGCAGGCGCGGGGCCGTCTGCCC10.53475935828877No Hit
TTTTACTACAGATGGTAAATTACCTAAGACAGAATATATCGTTATGAAAG10.53475935828877No Hit
GAGACGGAGTTTTGCCATGTTAGCCAGGCTGGTCTGGAATGCCTGACCTC10.53475935828877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)