FastQCFastQC Report
Tue 10 May 2022
EGAF00003629611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928427
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG33210.3577017902322961No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC32670.35188550096022625No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG32530.35037757411191184No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG25680.27659686760509977No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA22520.24256080445743175No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG22320.24040662324555404No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC21310.22952800812557156No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT20200.21757230239965014No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG19910.21444873964242744No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT19700.21218684936995585No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT19420.20917099567332706No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT18950.2041086698254144No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA18680.20120052518937948No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA17590.18946023758464586No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA17540.1889216922816764No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT16880.18181289428247993No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA16860.18159747616129218No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT16770.1806280946159472No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC16570.17847391340406946No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC15590.1679184254658686No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC15350.16533340801161536No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA14390.15499333819460226No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC14140.15230061167975512No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT14070.1515466482555979No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA13880.14950017610431407No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA13860.1492847579831263No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC13780.1484230854983752No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG13710.147669122074218No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC12990.1399140697114582No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT12760.13743676131779883No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA12720.13700592507542328No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA12610.13582112540889052No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC12570.135390289166515No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT12180.1311896358033534No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA12140.13075879956097786No Hit
TTACTATTCTGCCTAGCAAACTCAAACTACGAACGCACTCACAGTCGCAT11900.1281737821067246No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC11890.1280660730461307No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC11460.1234345834405936No Hit
GGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAA11240.12106498410752811No Hit
CCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATAC11210.12074185692574646No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC11110.11966476631980758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11070.11923393007743205No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT11050.11901851195624427No Hit
TACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGC11040.11891080289565038No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC10960.11804913041089929No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG10580.11395618610833162No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT10400.11201742301764166No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT10390.1119097139570478No Hit
AAATAGGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGT10280.11072491429051504No Hit
GGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCT10250.11040178710873337No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG10210.10997095086635782No Hit
ATCCTAGGCCCACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT10160.1094324055633884No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG10150.10932469650279451No Hit
ATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCC10140.10921698744220062No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT10070.10846302401804342No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT10030.10803218777566788No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG10020.10792447871507398No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC10010.10781676965448012No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG9850.10609342468497793No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT9680.10426237065488186No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT9640.10383153441250632No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC9550.10286215286716134No Hit
GTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT9520.10253902568537969No Hit
AGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCC9450.10178506226122248No Hit
AGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAACTTC9440.1016773532006286No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG9430.1015696441400347No Hit
GGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGT9430.1015696441400347No Hit
GAGTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCC9340.10060026259468974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)