FastQCFastQC Report
Tue 10 May 2022
EGAF00003629612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928427
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40060.4314824967391082No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC34830.3751506580485057No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG32170.34650004793053196No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG31320.3373447777800516No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG27600.297277007239126No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG23460.25268545615325705No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA22680.24428414942693394No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC21900.23588284270061083No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT20520.22101899233865452No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT20360.21929564736915233No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA19550.21057121346104757No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA19370.2086324503703576No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT17070.18385936643376377No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA16990.1829976939490127No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT16450.17718140467694282No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC16090.17330387849556292No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA15940.17168824258665463No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT15740.1695340613747769No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG15590.1679184254658686No Hit
GGGCACTGGCCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCC15460.1665182076781481No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA15160.16328693586033152No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT14650.1577937737700433No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA13950.15025413952847128No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA13670.14723828583184245No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT13640.1469151586500608No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG13550.14594577710471585No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA13520.14562264992293417No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC13520.14562264992293417No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC13140.14152970562036649No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA13120.1413142874991787No Hit
GAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTA12540.1350671619847333No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT12330.1328052717122617No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG12270.1321590173486984No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC12240.1318358901669167No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC12220.13162047204572896No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC12120.1305433814397901No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC12120.1305433814397901No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA11800.12709669150078573No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC11800.12709669150078573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.1265581461978163No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC11440.12321916531940584No Hit
AAATAGGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGT11400.1227883290770303No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC11320.1219266565922792No Hit
GGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGT11310.1218189475316853No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC11000.11847996665327483No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG10860.11697203980496043No Hit
GAGTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCC10670.11492556765367659No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT10630.11449473141130105No Hit
GGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAA10630.11449473141130105No Hit
GCAGTAATGTTAGCGGTTAGGCGTACGGCCAGGGCTATTGGTTGAATGAG10600.11417160422951939No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT10450.1125559683206111No Hit
GAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGC10420.11223284113882945No Hit
ATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCC10370.11169429583586No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT10300.1109403324117028No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG10110.10889386026041896No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC10080.10857073307863731No Hit
ATCCTAGGCCCACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT9670.10415466159428798No Hit
GTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT9660.10404695253369409No Hit
GGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAACTTCTGAGGTAATA9640.10383153441250632No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT9610.10350840723072464No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT9590.10329298910953688No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG9520.10253902568537969No Hit
GGTTTAGGTTATGTACGTAGTCTAGGCCATATGTGTTGGAGATTGAGACT9310.10027713541290806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)