FastQCFastQC Report
Tue 10 May 2022
EGAF00003629649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102047
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5040.49389007026174214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.4223544053230374No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA3370.33023998745676014No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGGCTGTCAATCTCGTATG3240.3175007594539771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.28418277852362145No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGGCTGTCAATCGCGTATG2750.269483669289641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.19304830127294287No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1760.17246954834537026No Hit
ATACACATCTCCGAGCCCACGAGACATGCGGCTGTCAATCTCGTATGCCG1720.16854978588297548No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1620.15875037972698855No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1530.1499309141866003No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1470.14405127049300812Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1360.1332719237214225No Hit
ACACATCTCCGAGCCCACGAGACATGCGGCTGTCAATCTCGTATGCCGTC1350.1322919831058238No Hit
ATACACATCTCCGAGCCCACGAGACATGCGGCTGTCAATCGCGTATGCCG1300.12739228002783032No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1220.11955275510304075Clontech SMART CDS Primer II A (100% over 26bp)
ACACATCTCCGAGCCCACGAGACATGCGGCTGTCAATCGCGTATGCCGTC1210.11857281448744207No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA1170.11465305202504729No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.10681352710025771No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1060.10387370525346165Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)