FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGAGACCCTTTGATGAGAAATTGGGTGAGGTATCTGAGATGAAA42.631578947368421No Hit
GGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGAGTTTGGATGA31.9736842105263157No Hit
GTGCTGACCAAGGATTTAATTTTTTTTTAATAAGGAGAGATGAAGTGAGC31.9736842105263157No Hit
AGTTAGGACAGGTGGCTTCATAAAGAAAGTGGCATAGGAAAATGGAAGCA31.9736842105263157No Hit
GCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGA31.9736842105263157No Hit
CACAAGCTACTTTCAATCTGAACTTCATTTGCCTCATCCCCAAAATGGGC21.3157894736842104No Hit
GCAACCTCAAGATGAAGAGCGGCCTGTTCGCGCAGGCGCTGCGCTGGGGT21.3157894736842104No Hit
AGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTACGTCTA21.3157894736842104No Hit
CGTGACCTTCGCGGCCGCCGAGGTGCAGCTCGAATTCATGGACCCCGCGG21.3157894736842104No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG21.3157894736842104No Hit
TCTCATCATTACATCTCATCTCATCTCATCATTTCATCATTTCATCTCAT21.3157894736842104No Hit
TTCCTACTCCTTCTCTTCCAGTGGCTGCTCTGGGTCCCTGAAAGAGGGTG21.3157894736842104No Hit
GGGGGGAAAAAGGAGTTGAAAGTAGGTCTTCATTTTGCAGTCATCATCTG21.3157894736842104No Hit
AAGCTGGACAGAGAATGACTTTGACGAGTTGAGAGAAGAAGGCTTCAGAT21.3157894736842104No Hit
GTCTCGATCTCCTGACCTCGTGATCCGCCCACCTTGGCTCCCAAAGTGCT21.3157894736842104No Hit
ATGCTTCTGTGCACAATGATTAAAAAATTCAACACAAACAGAATCCAAAA21.3157894736842104No Hit
GTCCCATTCAGGTGAGTTTGTACACACCATCAAGCAGCGAGCCTCTCATC21.3157894736842104No Hit
ACTCCAGGCTGGGCAACAGAGTGAGACTCCGCCTCAAAAAAAAAAAAAAA21.3157894736842104No Hit
CCTTATATATTCTGGTTATTAATCCCTTGTCAGATGGATAGTTTGCAAAT21.3157894736842104No Hit
ATTATGTCTTTTGTCTTTCCTTCTGTTAATGTGGTGTATCACATTTGTAA10.6578947368421052No Hit
ATATTAGCTTAACAATTTTAGTCGTGGCGTGGATCATTTTAGACTTTCTA10.6578947368421052No Hit
GGTATCAACGCAGAGTACATGGGAGACAGGAGATGTTGCCTAGTCTGCAT10.6578947368421052No Hit
ACATATATCTGAGGGTAGAGTTTTTTTGTGTGTTTGTTTGTTTTTTAACT10.6578947368421052No Hit
CATCAAGATTCAGCATTGAGAGATTTATTTAAAAAAATCTCAAGGATCAA10.6578947368421052No Hit
GATCAAATGGAATCGAATGTAATCATCATCAAATGGAATCAAAAAAAACC10.6578947368421052No Hit
GGGAAGCAGTGGTATCAACGCAAAGAAAAAAAAAAAAAAAAAAAAAAAAG10.6578947368421052Clontech Universal Primer Mix Long (95% over 22bp)
AATTCAAAGAGAGCAGTAGCCATATTAATTTGATGTGCCATTGCTCTGGA10.6578947368421052No Hit
ATGCAGGATTTCTTCACGCCTCCCCTTTGTGACTTCAAGAGCCTCTGGCA10.6578947368421052No Hit
GACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATT10.6578947368421052No Hit
GAGCTTTTCCCCTGTGTTTTTTTCCAAGTGTTTTATGGTTTCAGGTCTTA10.6578947368421052No Hit
AGTAATGTCTTTTACCTTCAGGTCCACTTTGTTTTATATTTTTTAAATGA10.6578947368421052No Hit
GGGTGTTGAACAGGGACCAGACGACATGGTCAGGCACGGAGGTCTCGCTG10.6578947368421052No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGG10.6578947368421052No Hit
AATTAAAGGTGACCATTTTGCAATTTCATCCCTCCAATCTTGATAACTTT10.6578947368421052No Hit
GTGCTGGGAAGGTGGAGGGAAAGACGCTTTTACCATCTGGATAGGACCAG10.6578947368421052No Hit
CAACATCACCGTGCCGTTCAAGCTCGAGGCCGCGGCGCTCGCGCAGCACG10.6578947368421052No Hit
GGAGAGAGATAGAAGTAATAGTTAAAGGGGTCCCTGAGGTCAAGGGATTT10.6578947368421052No Hit
TTCCAGACGCTGTTTCAGCGCGTCGCTAATCTGCTGTCTCTTATACACAT10.6578947368421052No Hit
CACCAGCAGACACACTAAGATACATGCAGGCCTGACCCCAGAGCGGATGA10.6578947368421052No Hit
CTCCACACAGCAGCCAGAATGTTACTTTTCAGAAATTTTTATTTTAATCA10.6578947368421052No Hit
TCATGAGGTCAGGAGATCGAGTCCATCCTGGCTAACACGGTGAAACCCCG10.6578947368421052No Hit
GTTGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAATTATTTATATTT10.6578947368421052No Hit
GGCCTTAGCTGTGCTCGCCCTACTCTCTCTTTCTGGCCTGGAGGCTATCC10.6578947368421052No Hit
ATACAGCAGACGGGACCTTTTTGGTACGAGATGCGTCTACTAAAATGCAT10.6578947368421052No Hit
CTTGACCCTTCTGAAGTAAATTGAAAGCATTTCCACAGTGTTCACACTAG10.6578947368421052No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGC10.6578947368421052No Hit
CCAATATATCTGATGAATATTGATGCAAACATTCTGAACAAAATACTAGC10.6578947368421052No Hit
ATGTATGGATGTACAAGAGATTTGTTTAACCATTCACCTACTGAAAGGTA10.6578947368421052No Hit
CCTCACTTCCGTCTTTTCAAGGTGAATTTTTCGAAGTATACAACTGCAAG10.6578947368421052No Hit
CTACTCAGGAGGCTGAGGCACTAAGAATTGCTTGAACCCAAGAAGCAGAG10.6578947368421052No Hit
CAGCTGCACTCAGAAGAACCTGTCGCTTCACTTGAAAACTTTCTGTTATA10.6578947368421052No Hit
CACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGT10.6578947368421052No Hit
GTCCTATATCTAGTAAAGAAATTTAATTGATAATTAAAACTTTTCCAGAA10.6578947368421052No Hit
GCCAAACCGGGGGGCAGGGACTGCTTCAGTTTCTCTGCCTTCTCTTTGTC10.6578947368421052No Hit
GTATGGTAGTTTTATTTTTAATTCATTAAGGAACATCCATACTGTTTTCC10.6578947368421052No Hit
TCACTGTGTTAGCCAGGATGGTCTCTATCTCCTGACCTCGTGATCCACCC10.6578947368421052No Hit
TTAAATAGGTTATTTGCATTTGAATGTATATATTTTTGTCCTTGATCTTG10.6578947368421052No Hit
CTGTATTAGACAGCATGAATAAAACATGTCCATCATTGCAGAAAGTTTCA10.6578947368421052No Hit
ACACAAGTTCATCAGCAGAGAATTTGAGAACGGCAGGATTATTTTAAATT10.6578947368421052No Hit
TTTAAAAATTTTTTTGTAGAGATGGGTGTCTCACTATGTTTCCCTGGCTG10.6578947368421052No Hit
GTGGAGCGATTTGTCTGGTTAATTCCGATAATGAACGAGACTCTGACATG10.6578947368421052No Hit
TCTCAGCACTTGGGAGGCTGAGGCGGGCGGATCACGAGGTCAGGAGATCA10.6578947368421052No Hit
GATTATTTTATCACCCAGTTATGAAGCCTGCCTTAATACCCTTTAGCCAT10.6578947368421052No Hit
GGATGAGAGGGTCAGAAGTCAAAGGTCCAAGCTGCAGGAGGGTCAGCCCC10.6578947368421052No Hit
GGTAAAATAGCGCAGCTTTAAATTTCATTTGATTTAGATTGAAAACTGTG10.6578947368421052No Hit
GAACCACAGATGGTAGCTTCGCCCCATTGACTCCTCAGCCAAGCACATAC10.6578947368421052No Hit
TCGAATGGAATCGAATGGAATCATCGAATGGATTCGAATGGAATAATCAT10.6578947368421052No Hit
CTGCTGGAGTGCTAGTTAATTAAAAATGGGCTTGTTAGGGGTGAAAATAT10.6578947368421052No Hit
CCAGTACAATATGCTGCAGCATAATTTGTCAGGCCAACCTTCACACCATA10.6578947368421052No Hit
GTTCAACTCTGTGAGATGAATGCACGCATCACATAGAAGTGTGAGATTAA10.6578947368421052No Hit
CTGCATGCATGAGGACACTGAAAGGGAGATTGTTCTTCTGGACAGGCCTC10.6578947368421052No Hit
GAACTGAGAAATTAAGTGCTTTCAATGTTGTAAATGTTTAGTGAGTGTCC10.6578947368421052No Hit
TCCTTATCACATTCTTGTCAATAGGGAAAAAAGAGAAAGCTTCAGAAACC10.6578947368421052No Hit
AATGAAGTTAAGCCGCCAGTTCACCGTGTTCGGCAGTGCGATCTTCTGTG10.6578947368421052No Hit
AATTAGAAGAAGTGTCTGACTGTTGAGAACAGATGCAGCCAAGGTGTCAC10.6578947368421052No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG10.6578947368421052No Hit
AATATATTCCTATAACTGTAGAACAGTGTGGAAAGTGATGGTAACTTTGA10.6578947368421052No Hit
GACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACA10.6578947368421052No Hit
TATCACATCTGTTTGCCATTCTGTGAAACACACAATCATAACAGATTTTC10.6578947368421052No Hit
ATATATAATATAATGTACTACATGCAATTGTTATGTACAGAGGATGAAAT10.6578947368421052No Hit
ATTCAGTATCTATTTTTTGGCATTATCCGTAATGCCTTGTACTGATGCGT10.6578947368421052No Hit
CTGCTAAACAGTTTGTGGTTTAGCTCCCAGGAATTAATTATTTGAGTACA10.6578947368421052No Hit
GAAATGCTATCATCGAATGGACTCGAATGCAATCATCATTGAATGGAATT10.6578947368421052No Hit
CATCGAATGGAGTCTAAAGGAATCACCAATGAAATGAATCGAAAGGAATC10.6578947368421052No Hit
CGCTCGTCCTCGTCGGTCAGCGCCACGGTCTGCTGCGCGGCGGTCTCGAT10.6578947368421052No Hit
GTGTTAATATGCATCCCTTCCCAACCATCTTACTTCTGAATTCTAATTCT10.6578947368421052No Hit
TGTATGTCTTCTTTTGAGAAGTGTCCATTCTGGAAAACAGTATGCAGGCA10.6578947368421052No Hit
ATCCTACATTCAGGGCACATTGGTGCAAAAGGTCAGCTCCCAAAACCTTG10.6578947368421052No Hit
CATTTGATAAAGCCATATTTTAAGTAATCTTCATTATAACGTCTTGCACT10.6578947368421052No Hit
TCTCACTTATCTTCCAGATTTTCCTGCCTGTAACCTTTCACCACCCGAAT10.6578947368421052No Hit
ATAAAGAACAGAATTTTTTTTTTTTTTTTTGAGACAGAGTTTTGCTCTTG10.6578947368421052No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA10.6578947368421052No Hit
CTATAAGAAAGAGAGACCAGAACTGAACTCCACAGGAACAAAAGGAAAGG10.6578947368421052No Hit
AGATTATACTGTTATGCACTGGCCATTAAGTCTTCACTGTTGACTTTTGC10.6578947368421052No Hit
CCATATGTACATAGAAAACCTGACAAATAATAACAAAAACAAAAGCTAAC10.6578947368421052No Hit
ACATAAATCTGAGGGTAGAGTTTTTTTGTGTGTTTGTTTGTTTTTTAACT10.6578947368421052No Hit
CCTTTAGGTCACTCTGACAAAGGCTTGGAGGAAGATTTATGGTACCCATG10.6578947368421052No Hit
CGTGCAGGTCGACCAGCTGCGTCTTGCACAGCGTCTGCAGGATCTGCTGC10.6578947368421052No Hit
CAAGAGAAAGCAGGAAAGATCTAAAATTGACACCTTAACATCAAAATTAA10.6578947368421052No Hit
AGTTTTGTCATGACTTTCTGACCTATATTTCACAATATTCCAAAATTATA10.6578947368421052No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAACCAAATTAT10.6578947368421052No Hit
CCTAGCTACAGAAAATTATTTAAGAATAAAACTAGATGTAAGCAAATAAA10.6578947368421052No Hit
GCTTCATGGTGCGGATCACCGCGTCGAGCGACACGTAGTGGCTGCCGTCG10.6578947368421052No Hit
ACTTAGTCTTGCTGGTGGCCAGCCCCATCCTGTCATGACTGTGACTATAG10.6578947368421052No Hit
GCATCCAGCAACATAAGCGGAGGCATTTTCCTTTTCTTCGTGGCCAATGC10.6578947368421052No Hit
ATTATAGAGAGGTTACAATTTAGGGGTTTTGGTGTTTAGCGTTAGATAAA10.6578947368421052No Hit
CCGTGAGGGAGGCAAGAACTGCACGGCAGCACGGGCAAGCTGCCCTGGGC10.6578947368421052No Hit
AGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATC10.6578947368421052No Hit
CCCGGAGGTTCACCAAAGACCACCTCGGTAAACTGCCGCCCGTTCAACCA10.6578947368421052No Hit
CTCTTTTTTTGTTGTGTCTCTGCCCAGCTTTGGTATCAGAATGATGCTGG10.6578947368421052No Hit
CCTTTCTTCAGCAAACAGTCTGTGGGCTGCATCCACTGTCTGACAAGCCC10.6578947368421052No Hit
ATTTAAAAATATCTTCCTACTGAGCATATCCAGTTTCTGTTTCTTGGAAT10.6578947368421052No Hit
CCTGCAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGT10.6578947368421052No Hit
GTCTGTGGAGGTTTGCAGAGTTCCATCTGTACTTTAATGAAGCCAGTGTA10.6578947368421052No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6578947368421052No Hit
GTCACTGTCCAGTTCATGTGCACTCTTGAGATTACAGAAACAGCCTCTGA10.6578947368421052No Hit
GGAATCATCTTCGGATGGAAACGAATGGAATCATCATCGAATGGAAATGA10.6578947368421052No Hit
GTTCTGTCTAGGTTTTATGGGAAGATATTTCCTTTTCTACCATACGCTTC10.6578947368421052No Hit
CATCGAATGGAGTCTAAAGGAATCACCATTGAAATGAATCGAAAGGAATC10.6578947368421052No Hit
GCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGGATATTAAAGTTGCTG10.6578947368421052No Hit
CATTTATTATGTGCATTACTCATAAGAGTTCAATATTCTGGGTAGCTATG10.6578947368421052No Hit
GTGTAGGCACTCAAATGGTAGTTGCTGCTTTTATTTTAGTAGACAAATAA10.6578947368421052No Hit
AGCCTCTCCTGAACAAATATAGTCTCCTCCATAATATAATCTAACTTCTC10.6578947368421052No Hit
GTAGACACAAGGATCCTTTCCATTAGCAGTACCTACTTTCCACCTGAGCA10.6578947368421052No Hit
GCATTTGGGTTGGTTCCAAGTCTTTGCTCTTGTGAATAGTGCTGCAATAA10.6578947368421052No Hit
GAATTCTACCAGATATTAAAAGAATTGGTATCAATTCTTTTGACACTATT10.6578947368421052No Hit
CACCACACAATCACACCACACACAATCACACACCACACTCATACAAACAC10.6578947368421052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)