FastQCFastQC Report
Tue 10 May 2022
EGAF00003629834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97993
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54115.52182298735624No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT3250.3316563427999959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.3296153806904575No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC2700.2755298847876889No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2530.25818170685661224No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2470.2520588205279969No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2440.24899737736368924No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2220.22654679415876644No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC2040.20817813517292053No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT2000.20409621095384364No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG1900.19389140040615144No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG1900.19389140040615144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.1908299572418438No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1830.18674803302276693Clontech SMART CDS Primer II A (100% over 26bp)
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA1680.17144081720122864No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1650.168379374036921No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA1640.16735889298215179No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1630.16633841192738255No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA1620.16531793087261334No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT1620.16531793087261334No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG1600.1632769687630749No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1540.1571540824344596No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1500.15307215821538273Clontech SMART CDS Primer II A (100% over 26bp)
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1480.15103119610584428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.14899023399630584No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT1440.1469492718867674No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA1430.1459287908319982No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC1370.1398059045033829No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG1300.13266253711999837No Hit
GATAAGAACCCTCCAGCTCGGGGCCTCAAACCGATATTCCATGTGGGCCA1270.12960109395569072No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC1260.1285806129009215No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1240.12653965079138305No Hit
CCTTTAGGCTAGGCCTTATTAACCTCTCTCTCCTACATTTGCTATGCGTC1220.12449868868184462No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1210.12347820762707541No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT1200.12245772657230619No Hit
GTACAGAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTGCACCTGTTACA1180.12041676446276775No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1140.11633484024369087Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGCAGATAAGAACCCTCCA1140.11633484024369087No Hit
ATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGT1120.11429387813415245No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA1110.11327339707938322No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1100.11225291602461401No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG1100.11225291602461401No Hit
GTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGT1100.11225291602461401No Hit
GTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGCCCTAACTGCGCA1090.11123243496984478No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC1070.10919147286030635No Hit
GTACATGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCC1070.10919147286030635No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT1070.10919147286030635No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.10817099180553712No Hit
CCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGATCGCAGGTTGTGC1060.10817099180553712No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1040.1061300296959987No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA1020.10408906758646026No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1000.10204810547692182No Hit
ATACATAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGA1000.10204810547692182No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA990.10102762442215261No Hit
GTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTC980.10000714336738338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)