FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3124
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA130.41613316261203587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3841229193341869No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC110.35211267605633806No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA100.3201024327784891No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC90.2880921895006402No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC90.2880921895006402No Hit
TTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGAT80.2560819462227913No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC80.2560819462227913No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT80.2560819462227913No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA80.2560819462227913No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC80.2560819462227913No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT80.2560819462227913No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC80.2560819462227913No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC70.22407170294494239No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA70.22407170294494239No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG70.22407170294494239No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC70.22407170294494239No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT70.22407170294494239No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG70.22407170294494239No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA70.22407170294494239No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA70.22407170294494239No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT70.22407170294494239No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT70.22407170294494239No Hit
GGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCC60.19206145966709345No Hit
ATCTAGTGAAACCTTCAAGCATGAATTGTAGCTGACGGCAGGCCCCTAAA60.19206145966709345No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG60.19206145966709345No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC60.19206145966709345No Hit
GCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTT60.19206145966709345No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC60.19206145966709345No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA60.19206145966709345No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG60.19206145966709345No Hit
GAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTT60.19206145966709345No Hit
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAG60.19206145966709345No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA60.19206145966709345No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC60.19206145966709345No Hit
CTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTCAATATCTAAC60.19206145966709345No Hit
GTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGA60.19206145966709345No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG50.16005121638924455No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG50.16005121638924455No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA50.16005121638924455No Hit
TATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGC50.16005121638924455No Hit
CCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTT50.16005121638924455No Hit
ACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTT50.16005121638924455No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA50.16005121638924455No Hit
CATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAG50.16005121638924455No Hit
TATCAACGCAGAGTACATGGGGGAATTCATCTCCTCTAACTTTGGAGAGG50.16005121638924455No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT50.16005121638924455No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG50.16005121638924455No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA50.16005121638924455No Hit
GGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCC50.16005121638924455No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG50.16005121638924455No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC50.16005121638924455No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA50.16005121638924455No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA50.16005121638924455No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC50.16005121638924455No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA50.16005121638924455No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA50.16005121638924455No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC50.16005121638924455No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT50.16005121638924455No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT50.16005121638924455No Hit
CTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTC50.16005121638924455No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA50.16005121638924455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.16005121638924455No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG40.12804097311139565No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG40.12804097311139565No Hit
CTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGC40.12804097311139565No Hit
TTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAG40.12804097311139565No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT40.12804097311139565No Hit
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA40.12804097311139565No Hit
AAATTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAA40.12804097311139565No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT40.12804097311139565No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG40.12804097311139565No Hit
ATCTTAAATAGACGAAAAGGAGAAAAAGATGGCAAACAAAAAAAATCCGT40.12804097311139565No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT40.12804097311139565No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG40.12804097311139565No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC40.12804097311139565No Hit
CAACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGA40.12804097311139565No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA40.12804097311139565No Hit
CAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGC40.12804097311139565No Hit
ACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTC40.12804097311139565No Hit
GTCCAAACATCAAGTGTAAGTTTATCATAGTTTGCAACTTGGCCTACACG40.12804097311139565No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT40.12804097311139565No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC40.12804097311139565No Hit
GGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGG40.12804097311139565No Hit
CCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGG40.12804097311139565No Hit
ATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATT40.12804097311139565No Hit
CTGTAGCTGGTGCATGTACTCGACTGCCGCTGCATCTACTATAAGACTCT40.12804097311139565No Hit
AATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGAC40.12804097311139565No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT40.12804097311139565No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT40.12804097311139565No Hit
TGTCAATTTAAACGCTATTAAAACCTTGAAAAGTATATTCAATATCCCTG40.12804097311139565No Hit
TCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTT40.12804097311139565No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT40.12804097311139565No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT40.12804097311139565No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC40.12804097311139565No Hit
CCGACGCAGTTTTGCTCCTTGGATTCTGAGCCCGGGCATCGCCCCTCGTT40.12804097311139565No Hit
GCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCG40.12804097311139565No Hit
GGGATACAGGACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCGGACGGACT40.12804097311139565No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC40.12804097311139565No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT40.12804097311139565No Hit
TACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGA40.12804097311139565No Hit
GTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGC40.12804097311139565No Hit
ATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAAC40.12804097311139565No Hit
ATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACG40.12804097311139565No Hit
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT40.12804097311139565No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT40.12804097311139565No Hit
AATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGG40.12804097311139565No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC40.12804097311139565No Hit
AGACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGC40.12804097311139565No Hit
GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT40.12804097311139565No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG40.12804097311139565No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA40.12804097311139565No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG40.12804097311139565No Hit
GAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTT40.12804097311139565No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG40.12804097311139565No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA40.12804097311139565No Hit
ATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCT40.12804097311139565No Hit
ATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAA40.12804097311139565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)