FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTC37.894736842105263No Hit
GCAAGCAGAGGTGGTTGGCAAAGGCAAGGTGGCTGACGATCCGGAAGCTG25.263157894736842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT25.263157894736842No Hit
GGCCAGAGGGAGCCGGAAGACCTTAGTTGAGTGGATCCTTTGCTGCAGTT25.263157894736842No Hit
ATTCAGAAGTGATCACGGAACAACTCAAAGGCAGAGCAAGAAGGATGAGA25.263157894736842No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.631578947368421Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGATGGACTGACGGATAGCATAAATCTGGGCCACGTGACCACCACCCTT12.631578947368421No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC12.631578947368421Clontech Universal Primer Mix Long (96% over 26bp)
ACATTAATCTCATGATTCCCATAATTATGCTCTCAAACAGCCCAAGTGAA12.631578947368421No Hit
CACTTCTGGAAACTTTCATTTTCAGAAAATAAACTCATAAATGTAGAATG12.631578947368421No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12.631578947368421Clontech Universal Primer Mix Long (96% over 26bp)
CTCTAGGACAGAGTTTGGATTTGTTTATGTTTGCTCAAAAGGAGACCCAT12.631578947368421No Hit
AAATAAAATCAGGCTCTTTTATAAGAAGTATATATATATATATATATCTC12.631578947368421No Hit
AATCAATCATTTACTCTTCTGTTTCGGTTGCTTCACGGCCATTGCATCCC12.631578947368421No Hit
GTTGTGTATACATCTTGATCATTAGCAAGACATTAAGCTTTAACCATTAT12.631578947368421No Hit
TATCAACGCAGAGNACATGGGAAGCAGAAGACGGCATACGAGATCTTGCT12.631578947368421RNA PCR Primer, Index 28 (96% over 27bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA12.631578947368421No Hit
TTTTAGTAGAGAGGGTGTTTCGTCATGTTGGTCAGGCTGGTCTCAAACTC12.631578947368421No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12.631578947368421Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCCCGCGAAAAAAAA12.631578947368421No Hit
ATGCTGCTTACATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAA12.631578947368421No Hit
CCAGGGGAGTCAGGCTGTCTGTGAAAGCCATGAGCTTCTCTCCCTCTCCC12.631578947368421No Hit
CATCTACAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAACAGCG12.631578947368421No Hit
GCCTGTAATCCCAGCACTTTGGCAGGCCCAGGCGGGTGGATCACGAGGTC12.631578947368421No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATAAACGCAGATTACATG12.631578947368421Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12.631578947368421No Hit
TAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGT12.631578947368421No Hit
TTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT12.631578947368421No Hit
GTGTCTACTTCTGCCACCTGGACATCATTTGGGTCAACACTCCCGAGCAC12.631578947368421No Hit
CTACTTGTTATTGGTTTATTCAGGGTTTCTATTTTTTCCTGGTTCAATTT12.631578947368421No Hit
AACTACAGCTGCGCCGAAGACGTGCTCGCGGGCGTGACGCTGGCCCGCGC12.631578947368421No Hit
GGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCG12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)