FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGAGC37.894736842105263No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25.263157894736842Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTTACCGATCAAATCTCAATGGTCGCTATGCAGTGTCTGAGGCTGCCG25.263157894736842No Hit
GAAGGCTCAAGCACAGCCAGTTATGTAAATGTATCTATCCCAATTGAGAC25.263157894736842No Hit
ATACATCTTACCCTCTATAGACCAAAACTAAAGCTAACACCCTTATCATA25.263157894736842No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA25.263157894736842No Hit
AAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT12.631578947368421No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT12.631578947368421No Hit
GTGCTGCAAAGCATCCGGAGAGCTTCGCCGCAGCAATGGAGCTGGAACGC12.631578947368421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCATG12.631578947368421No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12.631578947368421Clontech SMART CDS Primer II A (96% over 26bp)
GATATACGGCGACCACCGAGATCTACACTTTTTTTTTTCGTCGGCAAAAA12.631578947368421Illumina Single End PCR Primer 1 (96% over 27bp)
TACTCACGTCATCCAGCAGAGAATGGAAAGTCAAATTTCCTGAATTGCTA12.631578947368421No Hit
ATTGCCGCGTTTGCAGTGCGCCAGTCTTAGGACGCAAGAAGACAGCGACA12.631578947368421No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGTTAAAAAAAGACCCCCAGG12.631578947368421No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG12.631578947368421No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT12.631578947368421No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12.631578947368421Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA12.631578947368421Clontech SMART CDS Primer II A (100% over 21bp)
TCTGAACCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTCCCA12.631578947368421Illumina PCR Primer Index 6 (96% over 27bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA12.631578947368421Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTC12.631578947368421No Hit
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.631578947368421Clontech Universal Primer Mix Long (96% over 28bp)
CCTATATTCAAAAGATAGATCAAAAATTAACAGTCTAAGGTCACGCATCA12.631578947368421No Hit
GGCCAAGGCAGGTGTTTGAGGTCAGGAGTTTGAGACCAGCCTGACCAACA12.631578947368421No Hit
GGGAAGTAGAGCACTATGTGTCAGTATGCTTTGTTTTCTGACACATAGTG12.631578947368421No Hit
GTACATGGGATTCCTGTTGTCCTACCACCCCTTGCTCTTTGAGGGCTGGC12.631578947368421No Hit
CAGCGAAACGCATGCCTCCGAGGAGGCCTACGCGGCAGCGGCGGCGGCTT12.631578947368421No Hit
GTTCTGTACTAACTGGAATGTGTGTATGAAGTCTAATGAAAAGAATTAAT12.631578947368421No Hit
CAAATGGAAAGTACATTGAAGTGTGGGAGCTCAAATAGGCTCAAACTGGC12.631578947368421No Hit
GTCAAGGTACACTGCCGGAATTTCCAGAAAGAAAATGTGATTTTGCAAGT12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)