FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTACAGTGGGCCATGATCTCCCCCCAATCCCCTCCATTAATAGGAAT22.8169014084507045No Hit
GGCCAGACTCATTGGCAGATCTCAGTTTCCACAACATCCCCAGTGGAGCC22.8169014084507045No Hit
CTATTTGCAAAGTGTACACAGAATCATATGCAAATATATGTTTGTTAATG22.8169014084507045No Hit
GTGGTCAGCAAACTTTCTATAAAGGGCCAGAGTAAATATTTTAGGTTTTA22.8169014084507045No Hit
GTATAGTTTGAAGTCAGGCAGCGTGATGCCTCCAGCTTTGTCCAGCTTTG22.8169014084507045No Hit
AAATGGAAATATCTTCACATAAAAAACAGACAGAAGCGTTCTCAGAAACT22.8169014084507045No Hit
CTTCTGCCTCAGCCTCCCGAGTAGCTGGGATTGCAGGCGCCCGCCACCAC22.8169014084507045No Hit
AGTGTGTGCTGATGTTGGTTGTGGGAGAGATTTGAGAGCACTGTAATTTC22.8169014084507045No Hit
ACAAGGCCGTGCCCTGCGGCCCGATCAACGACATCGCGCAGGCCTTCGAC22.8169014084507045No Hit
AGCCAAAACACTCATTTGAATGAAATACATCTTTTTGCAGTATGACCTTC22.8169014084507045No Hit
GTATACGGAATGCTATCTGTCTCAGCCTTAAAACCAGTATTGCCGATTCT11.4084507042253522No Hit
AATCCAGCCCAGAGCACAAAGCCAAAGTGCTTTTGCAGCTCGAACCCTGC11.4084507042253522No Hit
GTTTTCTTTTACGTTGAAATGTAATTTGATGCAGAAAGAGGAAAGAAAAG11.4084507042253522No Hit
GAATGGAATGGAATGGAATGGAATGCAAAGGAATGGAATCAACCCGAGTA11.4084507042253522No Hit
GATCTTGGCTCACCGCAACCTCCACCTCCCGGATTCAAGCGATTCTCCTG11.4084507042253522No Hit
CACCCAAGCCCTGCGCCCCGCCCCTGGCCACTCAGCCACCATCTTGCCAG11.4084507042253522No Hit
GCATTTTTTCATGTGTCTTTTGGCTGCATAAATGTCTTCTTTTGAGAAGT11.4084507042253522No Hit
AAATAAATGAAATGACAAAAGATACTTACATGAGGCTAAAAAACACTTAT11.4084507042253522No Hit
GTTGTTTCCTGTATCAATGATTCATTCTTTTATGTTGCTGAGTAGTACTC11.4084507042253522No Hit
TCTCAGTGCTCTGAGAGTGGGGGCTCCTCCCCCGTTCAAGTGCTAGCCAC11.4084507042253522No Hit
CTCCCTTCGTGATCCCACTGGAGAGAGCTCATCCGTGGGGTCATTATCAT11.4084507042253522No Hit
GACACGGGTGAATGCTGCTAAAAACAAGACTGGGGCTGCTCCCATCATTG11.4084507042253522No Hit
CCACAAATGTTCTTTATTAAGAGAACCTTAATTGAGACCATTAAGCAGAA11.4084507042253522No Hit
CGATCACGGCGTCGAGCACCTCGCGGTGCAGCGGCAGCGAGAGCGCGGGG11.4084507042253522No Hit
CAGGAAGGGACTTAGAAACGCAAACTGCAGGCCCTGGAGTGTGCCCGACG11.4084507042253522No Hit
GGCTACTCTGAGCTATTTCCCAAAAGTCTGATCAACTCTGAAAGACCATA11.4084507042253522No Hit
TTTCACAAAATCCGCGCTCAGGCCAGGCTGCTCCCTTCTCTCTTCCCAAA11.4084507042253522No Hit
CACGTGGCCCGGCCGTCACACACGGCCCCAGGTGGGCCTCTTATTAGCTC11.4084507042253522No Hit
CTACAGGGGCAGGGATCCAGTTCACACATCACCAAGACAACAAACACCCA11.4084507042253522No Hit
TCCTCTGAGAGACCAGCCCGGCAGCTCACAGGGTGGGCAGATTGCTTTCC11.4084507042253522No Hit
CAAGTATCTGGGACTACAGGTGTGCACCATCACGCTCTGATTTTTTTTTG11.4084507042253522No Hit
GGCCTCCAGCTCCACCTGGCGAGGCTGGCCCATTGTACACAGGCGCCCCA11.4084507042253522No Hit
TTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATA11.4084507042253522No Hit
CTTGTTGACAAAGCCGCCGTGGTGGCCGACGACGTCGACGATCACCCGGA11.4084507042253522No Hit
ATGGAATATCCCGACTATGGAAGACTAAGCTGAAGGATGAGCATCAAGGA11.4084507042253522No Hit
TCCATACCCAGTGACCAGCAGCTGTCGTAGGCTGGGAGTAGAGATGTGGG11.4084507042253522No Hit
TGTGAGTATAGTGCTCACTTTTCTAAACTTAGCATCGGTTTTTGCACCTA11.4084507042253522No Hit
CTCCATGACGGCCAGGCCAACGGCAGCCTCCGCCTCTGCCAGCTGCTGCC11.4084507042253522No Hit
CGTCACCGACCAAGACGCCGGCCCAGGCGACGCGCGACACGGACACCTGA11.4084507042253522No Hit
CTGTCACCCAGACTGGAGTACAGTGGTGCAATCATAGTTCATTGCAGCCT11.4084507042253522No Hit
ATAATGAAGTGAGCAAAAATCACTGCAGATTGTCTAAGGCAAAGGAACCA11.4084507042253522No Hit
AAGCTGAAGCATGAGAATCACTTGAACCTGGGAAGCAGAGGTCCCAGTGA11.4084507042253522No Hit
GGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTATAG11.4084507042253522No Hit
GGTGGAGGCCCATGGCCCCTAGGGCACCACCCATAGTGGTGTTGCCCCCT11.4084507042253522No Hit
AGTGTCTGTTTTCCAAAAATCCAGAAACATGAGTCAGCTGTTCAACTATT11.4084507042253522No Hit
GGTAGGAGGTACCCCTTTTCCAGCCGGGATTTCCTCCCCCATATGGAGGG11.4084507042253522No Hit
GCCCTGCCCCGTGATGATGGCCACCCCCAGCCTGGGCAGGCCTTGCTCTG11.4084507042253522No Hit
GGATGGTGGCCGACCTGCTCGCGCAGGGCCGGCTGGTGCGCGTGCTCGGC11.4084507042253522No Hit
GGATAGCTTTGAAGATTTCGTTGGAAACCGGAATATCTTCATATAAAATC11.4084507042253522No Hit
TGCCTCTACCGTGGGGCTATTGAGATAAGATGTATCCTGCTGATATTAGC11.4084507042253522No Hit
CAGAAAACAGAAAGCTCCCAAATTTATTGTTGAGGTCAAAAGTATACTGA11.4084507042253522No Hit
CATATATATAATGAAATACCAAAAGGACTATTACTTATTTTTACAAAGCA11.4084507042253522No Hit
AGATCCTCCCGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCCGCC11.4084507042253522No Hit
ATGCGGCATACAGCAGCAGCGCGCCGAACAGCACCGCCGGAATCTGGAAG11.4084507042253522No Hit
GTACTCAACTTTAGGGCTTGAGAATTTCAGAAGCGCTCTTACTGAATCAC11.4084507042253522No Hit
TTACACAATAAAATGCAGTCACTTGTTTAATTACTTACCTCACCTATTGG11.4084507042253522No Hit
GTATAATGGTCCCTGGAAAGTGTTGTTCAACTTCATTGTTACAACACTGG11.4084507042253522No Hit
AAGAAGAAGAATCCTTAAGTCCCAACCAAACTCCTGCTGTGTCCACTGAG11.4084507042253522No Hit
ATTTATTTAACTTCACCCGAGTTCCTCTGGGTTTCTAAGCAGTTATGGTG11.4084507042253522No Hit
CTCAGCAACTGGGAAACGGGGGAGGGAGAAATTTTGGATTGAGGCTGCCA11.4084507042253522No Hit
ATTCAAGGCCGCACCTGAGCTTCCAGCCCCCACAGCCCCCCTGCCCGGCA11.4084507042253522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)