FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003629909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGGCGCCGATGAGCCACACCTTGGTGCCGCCCTTGGCGATGAACTCC27.4074074074074066No Hit
GCATATGGCTTGGCGAAGATATCTTGCGGCAACCCGAAAGCCGCCAGCAA27.4074074074074066No Hit
CCTCAGACTCACCCCATCACCTTGGCAGCAAAGCACTGGCTCTGCCGTCT27.4074074074074066No Hit
GTATCATCGGGGTGGTCGGCATTTTCGCGTACTATGTCGTGCGCACCTAC27.4074074074074066No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAACCACAG13.7037037037037033No Hit
CGCCTACGCCGAGGCCATCACCGAGTGCCGGGTAACGAAGGTGCCCAAGA13.7037037037037033No Hit
AATTGGACCCTTTCCATCAATAGCATTACCAAGGGCATCAACTACACGAC13.7037037037037033No Hit
TCCAAGTGCACTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATA13.7037037037037033No Hit
CGCGAGTGTGCGGCCGAAGCATGCGACGTGTACCTGATGTGGCCCGACAC13.7037037037037033No Hit
TGGTTATGGTGCTGGGAGAAAGGGGTGCACCTGGGAGGACACTGAGAAAG13.7037037037037033No Hit
GCCTGTCGGAGCTGGGTCTGGAAGAAGAAGCCAGCGAGCTGGAACAAAGC13.7037037037037033No Hit
CTCCTCTACGGACGTCAATTAGATTACCATGGCACAATAAGGCCGGAGCA13.7037037037037033No Hit
ACCGACGTCTTCCTGATGGTGGACACCGCCGCGATGTCGGCCAGGCACCG13.7037037037037033No Hit
CTTCCAGGAAGATGTGACTTGGGCACTAGGCTTTTTGTCTCACTTCTTAC13.7037037037037033No Hit
GTTGTGGCTCGCCTGGTGGTTGACGAGGTCGAGCGCGCGGTCGGCCTTCA13.7037037037037033No Hit
GCCATGTACAGAGAGCCAAAAGGACAGAAACAAACGTGATGTTGTGAGGG13.7037037037037033No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAAAACAG13.7037037037037033No Hit
ATTTAGAACCAAGCACAAATGATTTTGTTCTCTGAAAGCTGTTAGGTTAC13.7037037037037033No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC13.7037037037037033No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA13.7037037037037033Clontech Universal Primer Mix Long (95% over 21bp)
CGACAGCTCCGTTCTATGTCGCGAAAATCCTTCCGGCAGAGCTCGTCTGT13.7037037037037033No Hit
GTTCTACTCAGCCTCTGTGGAAGAAAACTTCCCACAGGGCTGCACTAGCA13.7037037037037033No Hit
TTATCGGAATTAACCAGACAAATCGATCCACCAACTAAGAACGGCCATGC13.7037037037037033No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)