FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003629910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCACCCGGTAAAGATCCCGAACAAAGCGGCCGCCGTGAAGAAGACCG27.4074074074074066No Hit
AGGTAGGTGCGCACGACATAGTACGCGAAAATGCCGACCACCCCGATGAT27.4074074074074066No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA27.4074074074074066Clontech Universal Primer Mix Long (95% over 22bp)
GTCCTCGATTTGACCGATGCCTCCCAGGCCGCGGCGCCCGCCGCGGCCGA27.4074074074074066No Hit
CGCCCAAGCCCTGTGCCTCCCTCCTCAAAGTGGGCATCCTACAGCCCAGC27.4074074074074066No Hit
CCCTTAGAGGGACTATTCCAACATCACTCCTTTGGAATTATTTCAGTCAT13.7037037037037033No Hit
GTGTCCCGCGGGGCCCGAAGCGTTTACTATGAAAAAATTAGAGTGTTCAA13.7037037037037033No Hit
TCCCGCTACGCGGCCCATGGTCAAAGCGTTGGCAACCGCATTGCCGCCGC13.7037037037037033No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13.7037037037037033Clontech Universal Primer Mix Long (96% over 26bp)
GCCGTCGCCATCCGCCGCGCCGCGCAGTTCCTGCTGGCGCTGGACGCCAT13.7037037037037033No Hit
GATCTGGACATGGGGGCCCGTCACCGGGCGGCGCGGACCGGCCAGCAGCC13.7037037037037033No Hit
CCCGCATCCTTGGTTAGCGCTTGCAGGAATTCCTGCTGCAACTCCGGATC13.7037037037037033No Hit
GGTGGCAGGTGTGATAATGATGTCTTACCTTGCAAAATAAAAGGATGATG13.7037037037037033No Hit
CTCATCACTCTGATCCCACTATCTATTCTGATTCAGCTTTTCATCTCTCT13.7037037037037033No Hit
GATAGACCCAAGAGGTGTTTTAGCTGTGCTGGAAGCACTTGTGGAAGAAC13.7037037037037033No Hit
GTCCTGGTGGGTTAGGCGGAGGTCTATGGTCACGGCCCCGAGGCCATCGT13.7037037037037033No Hit
TCCCTGCCCTTTGCGGGATAGACTTGTTGTCTCATCACCTGTCCTTGATA13.7037037037037033No Hit
ATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTA13.7037037037037033No Hit
CCATAAGGCTATACATGTATTACTTGACAGATTCTCGTTGTTTAGGTACA13.7037037037037033No Hit
GTGCGCGAGGCGCTGATGCGCATGGTGTCGATCGGCCTCATCGTGCAGCA13.7037037037037033No Hit
GTATTGGTGATGGTATTGCCCGCGTACATGGGCTGAGGAATGTTCAAGCA13.7037037037037033No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT13.7037037037037033No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)