FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003629922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003629922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTTGAGTTCACTGCATACAAAGCTTGACCATGAAGACATGCAGGTAAG24.545454545454546No Hit
GACCTAGAACGAAGCGCCGAGCCCAGGGCTTGGCGCGGAGCTCAATACGG24.545454545454546No Hit
TCGTAGAGCAGCACGTTTACCTGCTGCTCGTCCAGCAGGGCAGGCAGGCG24.545454545454546No Hit
TTTGAGGGGCTGGGAATAAAAAGAATATCAAGCAGCTTATAATCAATATT12.272727272727273No Hit
CCCAACTATGAGATGCTCAAGGAGGAGCACGAGGTGGCTGTGCTGGGGGC12.272727272727273No Hit
GCATATGACTGTGTGAGCACACTGGATAACGGTTGTTTCGCTCAGCCTGA12.272727272727273No Hit
TCACAAGTCCACCCTTTGTCAGCTTGGCACCTATACCTGTCTCCGTAAAC12.272727272727273No Hit
GTGTGTGTGTGTTCGTGTGTGTGTGTGGTTCTTGTTCCTAGCTGTCTCTT12.272727272727273No Hit
TCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCT12.272727272727273No Hit
CCCAAGAAGACAACGGAGATCAGAGACCACATCCCTCTTCCCTAATCAAA12.272727272727273No Hit
CCTCGAAGAACCTGCTGACCCTGCTCATGGACTCCATGATCGCTGTCTCT12.272727272727273No Hit
CCTGTCTACTGTTCTATCAATTGATTTCAAACCTTCAGAAATAGAAGTTG12.272727272727273No Hit
AGGTAGAGGAGCAAGACCTAGCAAAAGACTTAAAAAATGGGAGGTCAAAG12.272727272727273No Hit
GGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGA12.272727272727273No Hit
GACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTG12.272727272727273No Hit
GTTTTACACTTTAATAATGCTGGAGCGGCCTTACCACCGATGCCAGTTAC12.272727272727273No Hit
CCGCTGCCCCTCAGAAGAGGCCGCAGTCCTTCAGGTTGTTCTTGATGATG12.272727272727273No Hit
GTAGAAGAGGACCGCTGCACCCATCAGGCCTGCGCCGAGCGTGGGATTCG12.272727272727273No Hit
GTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTA12.272727272727273No Hit
AATCAAGGCCGCGGCGAAGCGTCGTGGCAGTGGCCTTCCATTCACCGGGA12.272727272727273No Hit
GCAGCAAAAAAGAAGTGACTCATTACATTCAAGGGATACTCACTAAGATT12.272727272727273No Hit
TGAAGGCTTGGAGCAACCGGATGCGATCTTCATCGGTGGCGGCGTCACCC12.272727272727273No Hit
ATGCTGCGCCGTCCCGGCCGCTGGGCCGCGCAATCTCCAGCGAGCATCCC12.272727272727273No Hit
AACCTAGACTCCTGTCTCTTTATCTTCTCCAGCTCATTCATTAACTCGTT12.272727272727273No Hit
ACCTTGCCCACAGCCTTGGCAGCGCCAGTAGAGGCAGGGATGATGTTCTG12.272727272727273No Hit
CCCATATTAAATACCAAAGATACAGGCTTGATCAAAACTCTCCCATCACT12.272727272727273No Hit
TTAACGAACAACGGCACCAGCAGCAAACGAATGAAAATCGTCAGACAGAC12.272727272727273No Hit
TCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGT12.272727272727273No Hit
CAAACTGCTGGGAAACATTACCTTCTGTGCTGCAGAGAATTCATGAGTTT12.272727272727273No Hit
CACCAGGACCCTCTCTTTTCCCTGCTTGTCTGCTAAGAGTCTGTGCAAGA12.272727272727273No Hit
GAGTGGGTCGCCGCACAAGGCTGGGACCTCGCGCCGCACCGCGCGCGGTT12.272727272727273No Hit
GCCGAGAACGCCGACGACGCCATGCGCCGCCTCGAGGAGGAGCACCCCGA12.272727272727273No Hit
GTACATGGGTGACCCTGTCTCCAAAAAACTGGAATAAACATTCTTAGCTA12.272727272727273No Hit
GGATACCTGAATAAAATTGGGAAAAGTTACAAAGAGAGGAATGGATGCTG12.272727272727273No Hit
CTTCTTTCCTTTTCCCTTCTGTTAAAACTCAATGTCCTTTCTTTTTTTTT12.272727272727273No Hit
GCCCAGGCCGGTCCCTCGCGCCCAGGTTCGAGCTCACATCACAGGGGCAG12.272727272727273No Hit
GGTGTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTG12.272727272727273No Hit
GCTGTGGGAGGCTGAGGCAGGAGAATCACTTGAACCTGGGAGGTGGAGGT12.272727272727273No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTCGTA12.272727272727273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACT12.272727272727273No Hit
GATCCAAAGAACTAGTAAAACTGGTAACAGGCCTATAAAAAAAGAGTTTC12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)