FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003630145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length100
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACCCGCGCGCCGCGCTCGACGACGCAATGGGCGCCGATGCGTGCAC33.571428571428571No Hit
GGTACGGAGACGTTGCCAGGGCCAGCTTGGTGGCGTTATCCATATGCGTA33.571428571428571No Hit
CTGCAGCAGCACGCCGCTGGCCGGCAGCGCGGGCAGCCGGTCCTGCACGG33.571428571428571No Hit
GTTCACGAAATGGCTGGGCGCGCCGCGGCTGATGGTGGCCGGCATGCTGT22.380952380952381No Hit
CCATGTGATGGTCCGGCCGCGACGGGATCAGGATGCGCACGTCCACCCCG22.380952380952381No Hit
CATCAGGTAGATCCGGGTCAGCTCCCGGGGGTGGGTTTGGGCCCAGTCCT22.380952380952381No Hit
TTCTAGCGTTCTATGAAGAAATCCCGTTTCCAACGAAGGCCCCAAAGAGG22.380952380952381No Hit
TCTTCGTACGTGCTGGCGTGGCTGCCGGACAGGATGATCGCCTTGGGCGC22.380952380952381No Hit
GACTGCACCATTACCATTGACGTCGGGTGGCAACGCATGGCTAATAGAAT22.380952380952381No Hit
GCCGCGTCATGATCGTGAGCGCCGAATCGGCCAGGGCCTCATCGAAGGTG22.380952380952381No Hit
GGCGATAGCCGCCCGCCCACGTAACCCACGACGGCGGCAACCAGCACGAT22.380952380952381No Hit
GATGGTGGCGATTCCCTTCCTGCTGTTCGACGGCCAGCTGTTCGGGCTGG11.1904761904761905No Hit
CGGTCTTGGGGTCACGCCTGCGGCGCACGAGACTCAACCAGCGAGTCAGG11.1904761904761905No Hit
GACACTGCCGGCAGCGGCAAGCTGATGGTGGCAGCCACGCCACTGGGGAA11.1904761904761905No Hit
TTCGTGAACGGCCCGGCCAGCAGGCCCTGGCCCAGCACCGACATCACGCC11.1904761904761905No Hit
GCACGCGGTGCACGCGCGGCGATTCCAGCGCGTTGCCCGGCGCCGTCAGC11.1904761904761905No Hit
AAGTAGTTGGCCGGCGAAACCACGCCCAGGTGCAACTGGCCGCTGAAGTC11.1904761904761905No Hit
GAAGCAGGCCGCGGCTGTAAGCGTCGTCGTAGTAGATGCCGGTGGTCTTG11.1904761904761905No Hit
ACCTTCTGCGATGCCGACATTGCGATCACCCGCCATCAACGACGGGTTGG11.1904761904761905No Hit
GGTTGAGGGTGGCGACGCTGACGACGATCGTCCCCTCATGTCGTCGATCA11.1904761904761905No Hit
CGGTACCAGTAGGCGACGAGGACGGTTTGGCCGTTGGCGGCCTCGATGAT11.1904761904761905No Hit
CTTCTCGATCAGCTCGCTGTACTTCTCGGGCTCGGCGGCGATGAGCTTGT11.1904761904761905No Hit
GGCCCGGGATGCCGAAGAAGCTCTCGAAGACGAGGCTGCCGAGGAAGACA11.1904761904761905No Hit
GTGTGCTCCGCGATCCGCTTTGCCGACCAGTTCGCGGACGAGGCGGGCGT11.1904761904761905No Hit
CGCCATCTTCGCTTTTTACGACCGTCGGATCCCTGACGGTATCGTCTCTG11.1904761904761905No Hit
GCGTCATCAGCGCGCGCACGCCGTGGCCCGCGCAGGCGGCGGCCACCTCG11.1904761904761905No Hit
GACCTGACGAGTGCAGACCTGAAGCCCGCCTTTGTCGGCGCGAGTTGCAC11.1904761904761905No Hit
GTAGTCGCACTCGATGAAGCGCTGGCCCTGCGCGTTGGTGGTGTAGCCGC11.1904761904761905No Hit
ACCTGCTCCAGCGAGGTTTGGTCGGTGATGTGGTGGTGGTTCGGGTAATG11.1904761904761905No Hit
CCGCACATCCACCGCCGCCTCCAGCCCCTGCGAATAGCTGAACGCGCCCA11.1904761904761905No Hit
ACCAGCCCCTTTGCCGCGCCCAGGGCCTTGCCGGGTTCGCCCGACGGCGG11.1904761904761905No Hit
GCCCAGCCACCCGCGGCGCATCGGCGTCGTCACGTCCACGGCCGGTGCGG11.1904761904761905No Hit
GGTCTGGGCCGGGCGGCATGGCGAAGCGACGCTCTCCAAAACCGGCCTCA11.1904761904761905No Hit
ACCGAGAAGCCCGCGCAACTCGTGGTTACGCCCGAGACGGCCTTCCCGAT11.1904761904761905No Hit
GTGGTGTGCGCGCATTGTCCGCGCGATTTGCGTTTTATGCAGGCAGCAGT11.1904761904761905No Hit
GCGCCGAGTAGACCGGTGATGACGCCGATCGGCAGCTCCGCCGGGCTGAA11.1904761904761905No Hit
CGTGAAACCTTCACGAGGCTGTCTGAGCACCGTCGAACAGTTCCCCTGCT11.1904761904761905No Hit
GTACGTAGGCTGTTTTATTTTTAAGAATCATGGTTCGAATAGCAATGGCA11.1904761904761905No Hit
ATCGCCACGATTGCGATGCTGCTGCCGGCCACGTACGCGCTGCCGCCGGT11.1904761904761905No Hit
TTCTCGACTTCGTCCGCCTGGGTTTTCCGGAAGCGAATGGGCGGCTGGAC11.1904761904761905No Hit
GTCACGGGCAGCACCTTCTCGCGCAACTCGCGATCGGGCGCGGTGCGGCG11.1904761904761905No Hit
GCTCCGGGGTGGGTAAAAGGAAACGGGTGAGATGACTCAGGCGGCCAGCG11.1904761904761905No Hit
ATGTTACATGAGCTGCTCGCCATTGCTCTATCTGTTTCAACGTAAGTTGC11.1904761904761905No Hit
GCACAATGCCGTGGCCGTCGATGGCGCCTTCCCAGGTGCCTGTGACGTGC11.1904761904761905No Hit
GCTGGAATTACATGCACACGCCACCACGCCCAGTTAATTTTTGTGTTTTT11.1904761904761905No Hit
AGCTCCTATCGTTCGTTCCTAATTAGTAAACAATCTCCTGTAACGTTGGT11.1904761904761905No Hit
GTTGTGTTGCGCATCCTATGATTTCTCGTAGAAAAAATCAACCGCCACTG11.1904761904761905No Hit
GCTACGCGCTGGTCATCGTGACCAACCAGTCCGGCGTCGCGCGCGGCTAC11.1904761904761905No Hit
CTGCAGACGCAAGGCTTCTCGATCACGCCGTTCCGCGCCGGCCACACCAC11.1904761904761905No Hit
CTCCCTAGAGGGCCCAACGGTCTCCAAGGGCAGAGCGTCTGGCCGCTGCT11.1904761904761905No Hit
CAGCGCATCGGGCGGGGCCGAATGGTCTTGAAGGTTGGACGGTGATCTAT11.1904761904761905No Hit
GGAATGGAATCACCCCATGTGGAATGGAAAGAATGGAATGGAATGGAAAG11.1904761904761905No Hit
GGCCGACCCACTGGAACAGCCGCTCCCGCTCGGGAATGCGTGCGGCCGAG11.1904761904761905No Hit
CTTACCGGCCGGCGTGCCGGTCGTAAAGACAAAAATGCCTCACCAATCGA11.1904761904761905No Hit
CCCCTACTCCACCACGGTCTTCTTGCCGTCGAAGACGTCGTGGTCGATGC11.1904761904761905No Hit
CACCAGTCTTGGTGTCGACGTCGTCAACGGCGCGGCTCGAATCGTCAGTC11.1904761904761905No Hit
ATACTGATCTGGTCGCCACGGTAGCGCATGATGTGGTTCCAGATGGCCTC11.1904761904761905No Hit
GATGCCGGCTTGCCGCAGCGCGGCGCGCAGCGTCAGCGCATAGGCGGTCA11.1904761904761905No Hit
GGGCCGCGCCGCGGCTGCGGGCGGGTCGGTTCAGATCCGCTGGTGCAGCG11.1904761904761905No Hit
GGGCGGCCTGCAGGGCCAGCAGGCTAAGGCCCAGGGAGGCAAGGGAGTCG11.1904761904761905No Hit
AACTTGAACCTGTCAGACCCGCTCAGAACCTGAGGTCGGGCAGGCTCAGC11.1904761904761905No Hit
CCGTATTGCCGACACCTCTTTGCCACTGGATGAACTGGTCGCCGATCCGG11.1904761904761905No Hit
CCATGAGACTGATATCGGAAATGTAGGCGAGGATCGCACGCTGCACAACA11.1904761904761905No Hit
AATCCTCGCCGTCGACGGCCGGCTTCGGCCCGCCGCACTGGCCACCTCTG11.1904761904761905No Hit
ACCCACAGCGGGGAGGCATCGTTGGCCCTCCAGAGGCGCCGGATGGCCTC11.1904761904761905No Hit
GCCGTTGGCGCGATCGCGTTGACGGTGATGTTGAAGCGCCCCCACTCGGA11.1904761904761905No Hit
GTTCTTGGCGTTGTTCTAGCCTTGACCGGCACTGCGGGAGACCTTGTCGA11.1904761904761905No Hit
GCCCTGGAGTGAGGTGGAAACACACCATGGCTCACGTGCCACATGAACAT11.1904761904761905No Hit
ATGCCCTGGCGCGAGCGCTCGAAGGCATCAACCAGTCCCGACGGCACGCG11.1904761904761905No Hit
GATCTCGCCGAGGATGCGCACGCCCAGGCCCGGGCCGGGGAAGGGATGGC11.1904761904761905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG50.094.05
TCCGGGG50.094.03
GTGGGTA50.094.09
GGTGGGT50.094.08
GGGGTGG50.094.06
CCGGGGT50.094.04
GCTCCGG50.094.01
GGGTGGG50.094.07
CTCCGGG50.094.02
GTCGGCC50.094.094
Produced by FastQC (version 0.11.5)