FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003630169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6412
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCACTTGGCGGATCTCGTATG731.1384903306300687No Hit
ATACACATCTCCGAGCCCACGAGACCTCACTTGGCGGATCTCGTATGCCG460.7174048658764816No Hit
ACACATCTCCGAGCCCACGAGACCTCACTTGGCGGATCTCGTATGCCGTC410.6394260761072988No Hit
TCTCCGAGCCCACGAGACCTCACTTGGCGGATCTCGTATGCCGTCTTCTG210.32751091703056767RNA PCR Primer, Index 23 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.3119151590767312No Hit
CATCTCCGAGCCCACGAGACCTCACTTGGCGGATCTCGTATGCCGTCTTC150.23393636930754835RNA PCR Primer, Index 9 (95% over 21bp)
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG130.20274485339987522No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA110.1715533374922021No Hit
CACATCTCCGAGCCCACGAGACCTCACTTGGCGGATCTCGTATGCCGTCT110.1715533374922021No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA100.1559575795383656No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA90.140361821584529No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA90.140361821584529No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT90.140361821584529No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT80.12476606363069245No Hit
GAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTT70.10917030567685589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGAGT602.746674E-1062.6666689
CGCAGAG602.746674E-1062.6666688
GTATCAA551.0370059E-859.818181
AACGCAG655.5842975E-1057.8461536
ACGCAGA655.5842975E-1057.8461537
ATCAACG602.0616426E-854.8333323
TCAACGC653.876812E-850.6153874
TATCAAC653.876812E-850.6153872
CAACGCA653.876812E-850.6153875
TACATGG951.6103513E-839.5789452
ACATGGG1053.8937287E-835.8095253
GTACATG1053.8937287E-835.8095251
CAGAGTA705.5837154E-726.85714310-11
AGAGTAC705.5837154E-726.85714310-11
AGTACAT659.825562E-625.30769312-13
GAGTACA652.9301314E-421.69230812-13
Produced by FastQC (version 0.11.5)