FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003630255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107813
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTACACTCGCTCATCTCGTATG5430.5036498381456781No Hit
CTTATACACATCTCCGAGCCCACGAGACTTACACTCGCTCATCGCGTATG4810.44614285846790275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.4220270282804485No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3730.34596940999693915No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3550.3292738352517785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.27454945136486325No Hit
ATACACATCTCCGAGCCCACGAGACTTACACTCGCTCATCGCGTATGCCG2730.2532162169682691No Hit
ATACACATCTCCGAGCCCACGAGACTTACACTCGCTCATCTCGTATGCCG2680.24857855731683565No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2640.24486842959568883Clontech SMART CDS Primer II A (100% over 26bp)
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2530.23466557836253513No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2410.22353519519909473No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2290.2124048120356543No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA2280.21147728010536762No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2080.19292664149963362No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG1880.17437600289389962No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1760.16324561973045923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.15582536428816562No Hit
ACACATCTCCGAGCCCACGAGACTTACACTCGCTCATCGCGTATGCCGTC1680.15582536428816562No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1660.15397030042759222No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1660.15397030042759222No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA1620.1502601727064454No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG1620.1502601727064454No Hit
ACACATCTCCGAGCCCACGAGACTTACACTCGCTCATCTCGTATGCCGTC1600.14840510884587202No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1560.14469498112472523No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1530.14191238533386513No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1510.14005732147329172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC1510.14005732147329172No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.13820225761271832No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1460.13541966182185822No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA1430.13263706603099812No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1370.12707187444927792No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG1300.120579150937271No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1290.11965161900698432Clontech SMART CDS Primer II A (100% over 26bp)
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC1280.11872408707669761No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA1280.11872408707669761No Hit
TAATAGGGCTGCGTGCGTCCTAAATACACTCGCTGTTGAGATACTAAAAT1230.1140864274252641No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC1220.11315889549497742No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA1210.11223136356469071No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1210.11223136356469071No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1160.1075937039132572No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT1160.1075937039132572No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1150.10666617198297051Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA1130.1048111081223971No Hit
GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC1130.1048111081223971No Hit
GCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGA1130.1048111081223971No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT1120.10388357619211042No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1120.10388357619211042No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA1090.1011009804012503No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC1090.1011009804012503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)