FastQCFastQC Report
Tue 10 May 2022
EGAF00003630411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164921
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3390.20555296172106644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT3210.1946386451695054No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT3140.1903941887327872No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC2890.175235415744508No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC2740.16614015195154042No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC2730.16553380103200926No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA2660.1612893445952911No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC2630.15947029183669756No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT2580.15643853723904172No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA2230.1352162550554508No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC2190.13279085137732613No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC2180.13218450045779495No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC2160.1309717986187326No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC2130.1291527458601391No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2070.1255146403429521No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT2030.12308923666482739No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG2010.12187653482576508No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG2010.12187653482576508No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1990.12066383298670272No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA1980.12005748206717155No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG1970.11945113114764037No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1940.11763207838904688No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG1920.11641937654998454No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC1900.11520667471092219No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT1840.11156856919373517No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG1820.11035586735467286No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC1780.10793046367654816No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT1770.10732411275701699No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT1740.1055050599984235No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT1710.10368600723982997No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT1710.10368600723982997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)