FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003630454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAATAAACTGAATGTTACATAAATAAGCTCAGGAACCAAAACAAGTTTC315.0No Hit
AAATTCTGCTTGCTTGCTTTTTAATATTGATATGCTTATACACTTACACT210.0No Hit
ATTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGGAGAAGG15.0No Hit
GTTCATAGGCGGCCAGCGACGCGTGGTCGCGGGCGCGGACGCGGTTGCGC15.0No Hit
CATTGGTTTCAAAGAATATCTTTATTTCTGCCTTAATTTCTTTATGTACC15.0No Hit
GAGCCACACTGAGCACGCTGTTTCTTAATCTGACTGGTCCCCATTTCTTC15.0No Hit
CCATTCCCCTGCACTCGGGTGGATTCCATTCTATTCCATTCCATTCCATT15.0No Hit
CCCCCGATCCTGTGTAGGCCTAGGCCAATATGTGTGCTTCTGTCTTCATT15.0No Hit
CCTGTACACGGGGGAGCTAGATGAGAACGAGCGTGACCTCATGCACATTG15.0No Hit
ATCTCATATTTCTTAATTTTTTGAGATGGAGTCTCGCTCTGTCACCCAGG15.0No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT15.0Clontech Universal Primer Mix Long (96% over 26bp)
GGGCTCGCCCGCGCCGGCCAGGCGTTGTGCCCGGGCGACATGATGGCATC15.0No Hit
GTGCTTCGGGTCAATCCCGTCAAGCAGCAGGTGCCGGTGCTGGTCGACGA15.0No Hit
ATAGAAAATTGACACCCTAACATCACAATTAAAAGAACTAGAAAAGCAAG15.0No Hit
ACATTCTACTCTGTGACTTGAATGAAAACATCACAAAGCAGTTTCTGAGA15.0No Hit
GTCCGGGCGGTGCCCGACACGCAGCTGAGTCCTCACCGAGCCGCCTAGGC15.0No Hit
AGATTATTCAAAAGATGGCAGAGGTTTTACAAATCATTTGGCTGTCTGGG15.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)