FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003630529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCGAAGAGCGCGCTGCCTTTCTCGTTCGCCATGCCGATGCGGCGAT215.384615384615385No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT215.384615384615385Clontech Universal Primer Mix Long (96% over 26bp)
ACATTTGAGTCCATTCAATTCCATTCCTTTCGTTTCCATTCCATTTGATG17.6923076923076925No Hit
CCTCGGCCACCACGGCTCGCCTAGGAGGACTTGATGGCTAAGGATTCGTT17.6923076923076925No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17.6923076923076925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGTATAACTGACCAGCCTGGCTTTCCCCCTCCTTTAGGAATCTGATTCT17.6923076923076925No Hit
CAGCAACCACCCACATTATTTTGCTGGACGTGTGTGCACAGGCACGTGTT17.6923076923076925No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT17.6923076923076925Clontech Universal Primer Mix Long (96% over 26bp)
ATTCAGTTTCAGTGTGTATTTTCAGGGAAAACGACTTAATTTAAGTATTG17.6923076923076925No Hit
GCTTAATATGGTTAGCACCTACTCCCATCCCAGTGGGTAAAACCAAAAAT17.6923076923076925No Hit
CTGCTGGCGCTGCACCGGACGGACCGATTCGACGCGGCCGCAGACCGCGC17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)