FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003630605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67.228915662650602No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC44.819277108433735No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT33.614457831325301No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG33.614457831325301No Hit
TCGATCAACGGTTGCTCGAGGAACGCGCAATGCGAGTCCTGTTCATGGAA22.4096385542168677No Hit
TCGTGCACCAGCGGCTGGCCGCGCGCGAGGCGCTGCAGGGCGCCTACGAC22.4096385542168677No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG22.4096385542168677No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACG22.4096385542168677No Hit
ACTTCGAGCTGATGATCATGACCGCCGACTCGCGCTTCGCCAACGATGTC22.4096385542168677No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.4096385542168677No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC22.4096385542168677No Hit
CTCTAAAGACCGTTACTTCCAGGACGACGTATTGGCTGAAGAAAAGCTAG22.4096385542168677No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.4096385542168677No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGTGTTGAT22.4096385542168677No Hit
CGGCAGCACGATGCGCCCGTCGAACATCGGCAGCACCATCGCCAGCGCCA22.4096385542168677No Hit
TCGCCCAACTGGGCGGCCTGTCGGACAAGCTGCACGGCGCGCTGGTCGGC22.4096385542168677No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG22.4096385542168677No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT11.2048192771084338No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA11.2048192771084338No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT11.2048192771084338No Hit
GGCTGAGAGCTCGCGCATTTCGCCAGCCACACCGCGAATTCCCGCGCCTG11.2048192771084338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11.2048192771084338No Hit
CAGCAACGTTATAACGGCCACTCAGTGGTTCGTCACGCGCAGCCCCGGGT11.2048192771084338No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.2048192771084338No Hit
GCAGCATCTCGAGGCGCGGCTGCAGCCAGCCTTCGGCGATCGGCGCCGGA11.2048192771084338No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.2048192771084338No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAA11.2048192771084338No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.2048192771084338No Hit
CAACGAAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA11.2048192771084338No Hit
ATCATGGCTTCGATCGATGGATCGAATTCCGCAGGAGGAAGGTATGGAGA11.2048192771084338No Hit
CTGCTGGGTCTCGCCGTTGCCGCGAAATGGATCGCCGCGCCGTTCGTCGC11.2048192771084338No Hit
GATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC11.2048192771084338No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.2048192771084338No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.2048192771084338No Hit
CGCTCACTCACTGTCAAAACTAACACCTTCACACCAGGCGTTTCCCTTTG11.2048192771084338No Hit
GAATACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGGGTTT11.2048192771084338No Hit
TTTTATCCCTTATTAGAAAAAGTGGCAAAAACAGGAGAGCCGGTGATTTT11.2048192771084338No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2048192771084338No Hit
AAAAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT11.2048192771084338No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT11.2048192771084338No Hit
TATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGC11.2048192771084338Illumina PCR Primer Index 1 (96% over 28bp)
ACAGATCGGCGAGGCCGTCGCTCAGGTTGGCGCGGGTCTTGCCCAGCCCG11.2048192771084338No Hit
AGCCACACCAACCCGGTCGAAAGGATCAACAGCAAGAAACCAAACGCCGC11.2048192771084338No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.2048192771084338No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGA11.2048192771084338No Hit
AATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.2048192771084338No Hit
GGCCGAAGCTGGAGGCCGAATCGCCCACCAGCACCGCGAGCGCATGCTCC11.2048192771084338No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.2048192771084338No Hit
GTGCTGACTTCGCCGGCACAACGCGCAGGGAGCGAAGCGACAGCTCGGCA11.2048192771084338No Hit
CGCGCGGGTCGCGAGCTCCTCGAGGGCCAGCGCGGCGATCCGGACGCGCA11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGTTTTTAAAA11.2048192771084338No Hit
ATTACAAACATTACCATAAAAAAAAAAAAAAAAAAAAAAAATAACCAGTC11.2048192771084338No Hit
GTACTCTGCGTTGATACCACTACGCAGAGTACTTTTTTTTTTTTTTTTTT11.2048192771084338No Hit
GTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTT11.2048192771084338No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11.2048192771084338No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCT11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT11.2048192771084338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGAGC50.094.03
GAGCTGA50.094.06
CTGATGA50.094.09
ACTTCGA50.094.01
GCTGATG50.094.08
CGAGCTG50.094.05
CTTCGAG50.094.02
GCCGTGA50.094.094
AGCTGAT50.094.07
TCGAGCT50.094.04
Produced by FastQC (version 0.11.5)