FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003630606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89.63855421686747No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67.228915662650602No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT33.614457831325301No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC33.614457831325301No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG33.614457831325301No Hit
CCTGGTATGCGCCCATCCTGGGCACGGTGGGGCTGTTCGTGATGTCCTTC22.4096385542168677No Hit
CCGCTACAGTCTGCTGAATGTCATGCGGATGACTTTCGCGCAGACCAGCG22.4096385542168677No Hit
CCAGGAACACCACCGCGTTGGCCGACAGCGTGCGCAGCGCGGCCAGCGGC22.4096385542168677No Hit
GTCGCATCCCCATCCAGGCCACGGTCTCCGGCCACGACATCGCAGCGATC22.4096385542168677No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC22.4096385542168677No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.4096385542168677No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA22.4096385542168677No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG22.4096385542168677No Hit
GGATCTGACATCGCACACTCCTGAAGAGGTCCGCTTCAGGGTAGGGATGG22.4096385542168677No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAAC11.2048192771084338No Hit
CTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.2048192771084338No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTAAAACAATGAAAA11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT11.2048192771084338No Hit
GTACATGGGAGTGGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAT11.2048192771084338No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11.2048192771084338No Hit
GTCGGCGGGCTCCCGGACCTGCGCGTCCGGATCGCCGCGCTGGCCCTCGA11.2048192771084338No Hit
GCGCAAAGGACGCGGCGTCGAATTGACGGTCTCCGGGCGCTTGCTGCTCG11.2048192771084338No Hit
TCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTGCC11.2048192771084338Illumina PCR Primer Index 1 (96% over 28bp)
GGGCACCCCAGGCCAGTGAAGCCCAACCGATCAGCACTGCGGTGTCCAGC11.2048192771084338No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.2048192771084338No Hit
GCATTGTATGTGTGGGCATGGCTATGGCAGCCATGATGCTCTGAAGGTCA11.2048192771084338No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.2048192771084338No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTCTCCTGTTTTTGCCAC11.2048192771084338No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTCGGGACATGTTGGTCCCAAAAC11.2048192771084338No Hit
GAGCGCAGGTCGCCGGGATTGCCTGCGCTGGCGGCGAGCGCGAAGAAATC11.2048192771084338No Hit
GATAGCGACTGGACACAACATGGCAGTTGGCCCACATGGAATATCGGTTT11.2048192771084338No Hit
CTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2048192771084338No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.2048192771084338No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACCACTGCTTCCTTGTACT11.2048192771084338No Hit
GTTCATGGTCGTGCCGCTCGTGCACAGCCTCGAGACGCTCGACGACAGCC11.2048192771084338No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.2048192771084338No Hit
CTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAA11.2048192771084338No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAA11.2048192771084338No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCT11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATAAACGCAGAGTACATTGGAAGCCTGTCTC11.2048192771084338No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCGGT11.2048192771084338No Hit
CCTCTCGGGCGCGAGCAAGGCGACGAACCTGGACGCGGCCGGCCGCGAGG11.2048192771084338No Hit
AGCATGATGTGGATCTTGTGGCCGTTGGGCGTGGGCCAGGAATAGACGTC11.2048192771084338No Hit
CTCATGCGCACCGAAGCCCCGAGCGGCACGCCGCAGCAGCTCGGCGAGCA11.2048192771084338No Hit
GGTAACAACGCAGAGTACATGGGAAGCAGTGGTAACAACGCAGAGTACAT11.2048192771084338No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTTAAC11.2048192771084338No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.2048192771084338No Hit
GAAAAAGGCGGGGGCGAAGGTCGCGAAATAGGTGGCGATGCTGACCGTCC11.2048192771084338No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.2048192771084338No Hit
ACGCTCTTCCGATCTCTGTCCCAAGTTCTGTAGGTACCAGAGATCGGAAG11.2048192771084338No Hit
GGGCACCCCAGGCCAGTGAAGCCGTACCGATCAGCACTGCGGTGTCCAGC11.2048192771084338No Hit
ATCCTCTCCGAGGCCGCCGAGGAGGCCCACGAGGAAGTGGACCGCCAGGA11.2048192771084338No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA11.2048192771084338No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.2048192771084338No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGT11.2048192771084338No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2048192771084338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCG50.094.094
TGACATC50.094.06
GGATCTG50.094.01
CTGACAT50.094.05
ACATCGC50.094.08
GACATCG50.094.07
GATCTGA50.094.02
TCTGACA50.094.04
ATCTGAC50.094.03
CATCGCA50.094.09
Produced by FastQC (version 0.11.5)