FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003630791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520198
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATAGATTCTCTGGCACTCTGAGGTCTCTGTGGCAGGCCTGGTCAGG67030.2659711657576111No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT55330.2195462420016205No Hit
ACTTCAGACATCAAATCCTGCAGACAAGGGGAGCCCTCAGTCTGCAGGGC51380.20387287030622198No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG50550.2005794782790876No Hit
GACAGGATCCTCCCTGTCCCATGGCCTAAATGCCTCTCAGAACTCACAGA41620.1651457544208828No Hit
CTCCTAGCCTTGGCCTTTGGCATGAGATGGAGGCCTCCTTATTCCCCATC41420.16435216598060945No Hit
GGACGCTCTCTGGCCTTCGGCCTCCATCCTGAGGCTCCACTTGGTCTGTG40290.15986839129306507No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG37720.1496707798355526No Hit
ATTGGGACTGGAGGCACCCTTAGCCACTTCATTCCTCTGATGGGCCCTGA36510.14486956977189888No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG36340.14419501959766653No Hit
GGTTCTTGGCAAATGATAGGTGCTCAGTCAATAGCCTCTGTGTATAGTAA36200.1436395076894752No Hit
GCTCCACACAGTCAGGGCTTCCCTGAAATCTGCCTCTCTCTGTCATTATG36170.1435204694234342No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT35580.14117938352462783No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG34860.13832246513964377No Hit
ATCTCCACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTG34560.13713208247923378No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC34180.13562426444271442No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT32690.12971203056267802No Hit
GGACGCTCTCTGGCCTTCAGTTTCCATCCCCAGGATCCACTTGGTCTGTG32600.129354915764555No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA30060.11927634257308355No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG29890.1186017923988512No Hit
TCTATACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTGC29590.11741140973844119No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG29420.11673685956420884No Hit
GTGCAGAGCTAAGGACACAGTGGAGATTCTCTGGCACTCTGAGGTCTCTG28930.11479256788553915No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA28900.11467352961949816No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG28900.11467352961949816No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG26830.10645988926266904No Hit
CTTCAGTTTCCATCCCCAGGATCCACTTGGTCTGTGAGATGCTAGAACTC25980.10308713839150734No Hit
GTCACAGGGTTGGCTTGGGCACCACCGTGCTGGGGCACCACTGGGCCCTC25530.10130156440089232No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG25290.1003492582725643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)