FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003630810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98727
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG2460.24917195903856088No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG2110.2137206640533998No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT1980.2005530402017685No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1930.19548856948960266No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG1900.19244988706230312No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG1790.1813080514955382No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG1780.18029515735310503No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA1710.1732048983560728No Hit
GCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAAT1560.1580114862195752No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA1550.156998592077142No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1510.1529470155074093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1450.14686965065281027No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT1390.14079228579821124No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC1380.13977939165577805No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA1370.13876649751334488No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1340.13572781508604537No Hit
GTGCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTA1330.13471492094361218No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA1320.133702026801179No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA1290.13066334437387947No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG1260.12762466194657995No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTT1260.12762466194657995No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC1240.12559887366171363No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1230.12458597951928044Clontech Universal Primer Mix Long (96% over 26bp)
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1200.12154729709198092No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT1160.11749572052224821No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT1160.11749572052224821No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA1140.11546993223738188No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT1140.11546993223738188No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT1130.1144570380949487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.11344414395251551No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT1090.11040546152521599No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1090.11040546152521599No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT1080.10939256738278283No Hit
TTACATAACAGACGAGGTCAACGATCCCTCCCTTACCATCAAATCAATTG1060.10736677909791648No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC1030.10432809667061695No Hit
GTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCG1030.10432809667061695No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGTCCTATTT1030.10432809667061695No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT1020.10331520252818378No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG1010.1023023083857506No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG1010.1023023083857506No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA1010.1023023083857506No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT1010.1023023083857506No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA990.10027652010088425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)