FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003630813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003630813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2710.074626865671641Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT238.582089552238806Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT124.477611940298507Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC72.6119402985074625Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62.2388059701492535Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG62.2388059701492535Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC62.2388059701492535Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51.8656716417910446Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA41.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG41.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC41.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT31.1194029850746268Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA31.1194029850746268Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA31.1194029850746268Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31.1194029850746268Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT31.1194029850746268Clontech Universal Primer Mix Long (100% over 23bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31.1194029850746268Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31.1194029850746268Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA31.1194029850746268Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA31.1194029850746268Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTAGTCTGCGTTGATACC20.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGCGCGATTAACAATCGACCTGCGCCATCCATCTGACATTCGCTG20.7462686567164178No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
TCTCACGCCCGACCGATCGAGCTCCGCGATGGCGCGCTCCGTGGTGCCTC20.7462686567164178No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.7462686567164178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT20.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.7462686567164178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGAAATCGAGATTTTGGAGAAACTGGTGCCAGAGGTTATGGAACACGCA20.7462686567164178No Hit
TGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCA20.7462686567164178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC20.7462686567164178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT20.7462686567164178Clontech Universal Primer Mix Long (96% over 27bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC20.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCCAGAGTACATG20.7462686567164178Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGG20.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCACTGCTTACCCATGTACTCTGCGTTGATACCACTG20.7462686567164178Clontech SMART CDS Primer II A (95% over 23bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.7462686567164178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC20.7462686567164178Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTA20.7462686567164178Clontech Universal Primer Mix Long (95% over 21bp)
CGTCACCGCACCCACTGGCTACAACGACGACGAGATCCTCGGCCCGATGA20.7462686567164178No Hit
AAAAAAGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATCTGTCTCTTATACAC10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATACCAATGC10.3731343283582089No Hit
GGTATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGG10.3731343283582089Clontech SMART CDS Primer II A (100% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACCTGTCTCTTATAC10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTAAACCACTGCTT10.3731343283582089No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAA10.3731343283582089No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCAA10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
GATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGG10.3731343283582089Clontech Universal Primer Mix Long (100% over 26bp)
GCGTTGATACCACTGCTTCCATTTACTCATCCGTTGAAACACTTCGTGCC10.3731343283582089No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACGCTGCGTT10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA10.3731343283582089Clontech Universal Primer Mix Long (95% over 21bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCCAGT10.3731343283582089Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTC10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTAT10.3731343283582089Clontech SMART CDS Primer II A (100% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATA10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.3731343283582089Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCCGCGGAAAAGGGAAGCAGTGG10.3731343283582089Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.3731343283582089Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCT10.3731343283582089No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGAATCAACGCAGAGTACATGGGAAGCAGTGGTA10.3731343283582089Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCACCAGGTCTTTCTTGAACCTGAAGGACTGGATACGCATCTGGTCTACC10.3731343283582089No Hit
CTATAAGCGCTGTCACGGATGTATAAATCGCTCGAGAATCACACACAGTC10.3731343283582089No Hit
GTTGATACCACTGCTTCCCATGACTCTGCGTTGATACCACTGCTTCCATG10.3731343283582089Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTCT10.3731343283582089Clontech Universal Primer Mix Long (95% over 21bp)
CACCTGCGGGGACGTGGAGGTCTTGCTCACTTCGTCAGTCCTGCCCGCTG10.3731343283582089No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTAACCATGTACTCTGCGTTGA10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATTTACACTGCGATGAA10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTCGGCTCGTCGGCGATGATGAGGTCAGGGTCGTTCGCGATGGCCAT10.3731343283582089No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCA10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CCTATGTACTCTGCGTTGATACCACTGCTTCCTATGTACTCTGCGTTGAT10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA10.3731343283582089No Hit
GCTTTCCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGC10.3731343283582089Clontech Universal Primer Mix Long (96% over 27bp)
GGCCGAATGACTCGGCGGCCGCGGGTTGGGTCTTCTTGGTTTTTGCTTTG10.3731343283582089No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAA10.3731343283582089No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGA10.3731343283582089Clontech Universal Primer Mix Long (96% over 27bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.3731343283582089Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGAGTT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.3731343283582089No Hit
CCCATGTACTCTGCGTTGATACACTGCTTCCCATGTACTCTGCGTTGATA10.3731343283582089No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC10.3731343283582089Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGATCAACGCAGAGTACATGGGAAGCAGTGGATCAACGCAGAGTAC10.3731343283582089No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.3731343283582089Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCAT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGAACTATCC10.3731343283582089Clontech Universal Primer Mix Long (96% over 27bp)
GCAGAGTACATGGGAAGCAGTGGTATAAACGCAGAGACCACTGCTTCCCA10.3731343283582089Clontech SMARTer II A Oligonucleotide (95% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.3731343283582089Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTCCTACTATATCGCGTATG10.3731343283582089No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.3731343283582089No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATAGACATCGTCGATCAGCAGCACGTGCCTGCCCCGGATGCGCTC10.3731343283582089No Hit
CCCATGTACTCTGTGTTGATACCACTGCTTCTCATGTACTCTGCGTTGAT10.3731343283582089Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTCTGTCTCTTATAC10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGTGTTGATACCACTGCTTCCCATGTACTCTGCGT10.3731343283582089Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAATGAAGGAGAGGGGTCAGGGTTGATTCGGGAGGATCCTATTGGTGAGG10.3731343283582089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.3731343283582089No Hit
CGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCG10.3731343283582089No Hit
GCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATACCACTGCTTCC10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTGCATGGGAAGCAGTGGTATCAACGCAGAGT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
CAGTAGATGCTGCCATTGAGGAATTAAGCAGAGTTTTAGGGCTGTCTCTT10.3731343283582089No Hit
GCCGCGGCCCTGTTCGCCTGCGCGGGCGGCTTCCACGGCGGCGTTCAGCG10.3731343283582089No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA10.3731343283582089No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT10.3731343283582089Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.3731343283582089No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG10.3731343283582089No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGA10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
GTACAAGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.3731343283582089Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGACT10.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAAAGCAGTGGTATCAACGCAGAGTACTTGGCTGTCTCTTATACACA10.3731343283582089Clontech SMART CDS Primer II A (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)